This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Phosphotyrosine Antibody, recombinant clone 4G10®
catalog :
05-777
quantity :
1 mg
clonality :
recombinant
host :
mouse
conjugate :
nonconjugated
clone name :
4G10®
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 123
Reference
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product information
Catalog Number :
05-777
Subcategory :
Cell Signaling
Product Name :
Anti-Phosphotyrosine Antibody, recombinant clone 4G10®
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Host Name :
Mouse
Antigen :
Phosphotyrosine
Clone :
4G10®
Conjugate :
Purified
Isotype :
IgG2bκ
Product Description :
Anti-Phosphotyrosine Antibody, recombinant clone 4G10®
Cross Reactivity :
Vertebrates
Background :
Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
Immunogen :
Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via Nickel affinity matrices.
Specificity :
Recognizes tyrosine-phosphorylated proteins from all species.
Package Size :
1 mg
Uses :
Immunoprecipitation;Western Blotting
Storage :
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

Hundreds of New Antibodies in the areas of:
  • • Cancer
  • • Nuclear Signaling
  • • Apoptosis
  • • Cardiovascular Disease
  • • Developmental Biology
  • • Metabolic Disease
  • • Neurodegenerative Disease
  • • Cell Biology
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