phospho-Histone H3 (Thr3) Antibody, clone JY325, rabbit monoclonal | EMD Millipore 05-746R product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-Histone H3 (Thr3) Antibody, clone JY325, rabbit monoclonal

catalog :

05-746R

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JY325

reactivity :

Saccharum sp., human, fruit fly

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; fruit fly ; 1:200; loading ...; fig 2a	Tarayrah L, Li Y, Gan Q, Chen X. Epigenetic regulator Lid maintains germline stem cells through regulating JAK-STAT signaling pathway activity. Biol Open. 2015;4:1518-27 pubmed publisher
immunohistochemistry; Saccharum sp.; 1:100	Moraes I, Yuan Z, Liu S, Souza G, Garcia B, Casas Mollano J. Analysis of Histones H3 and H4 Reveals Novel and Conserved Post-Translational Modifications in Sugarcane. PLoS ONE. 2015;10:e0134586 pubmed publisher
	Patnaik D, Jun Xian -, Glicksman M, Cuny G, Stein R, Higgins J. Identification of small molecule inhibitors of the mitotic kinase haspin by high-throughput screening using a homogeneous time-resolved fluorescence resonance energy transfer assay. J Biomol Screen. 2008;13:1025-34 pubmed publisher

product information

Catalog Number :

05-746R

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-phospho-Histone H3 (Thr3) Antibody, clone JY325, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P68431

Host Name :

Rabbit

Antigen :

phospho-Histone H3 (Thr3)

Clone :

JY325

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-phospho-Histone H3 (Thr3) Antibody, clone JY325, rabbit monoclonal

Cross Reactivity :

Human

Background :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis. Consistent with this role, Histone H3 phosphorylated at threonine 3 has been shown to be recognized by the BIR domain Survivin, a member of the inhibitor of apoptosis (IAP) family.

ALT Names :

Histone H3.1;Histone H3/a;Histone H3/b;Histone H3/c;Histone H3/d;Histone H3/f;Histone H3/h;Histone H3/I;Histone H3/j;Histone H3/k;Histone H3/l

Immunogen :

KLH-conjugated linear peptide corresponding to human Histone H3 phosphorylated at Thr3.

Specificity :

This antibody recognizes Histone H3 phosphorylated at Thr3.

Package Size :

100 µL

Uses :

Western Blotting;Peptide Inhibition Assay

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.