trimethyl-Histone H3 (Lys4) Antibody, clone 15-10C-E4, rabbit monoclonal | EMD Millipore 05-745R product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

trimethyl-Histone H3 (Lys4) Antibody, clone 15-10C-E4, rabbit monoclonal

catalog :

05-745R

quantity :

100 μL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

15-10C-E4

reactivity :

human, mouse, brewer's yeast

application :

western blot, ELISA, chromatin immunoprecipitation, ChIP-Seq

citations: 8

Published Application/Species/Sample/Dilution	Reference
ChIP-Seq; mouse; loading ...; fig 1b	Hainer S, Boskovic A, McCannell K, Rando O, Fazzio T. Profiling of Pluripotency Factors in Single Cells and Early Embryos. Cell. 2019;: pubmed publisher
ChIP-Seq; human; loading ...; fig 3a	Kelso T, Porter D, Amaral M, Shokhirev M, Benner C, Hargreaves D. Chromatin accessibility underlies synthetic lethality of SWI/SNF subunits in ARID1A-mutant cancers. elife. 2017;6: pubmed publisher
western blot; brewer's yeast; fig 6	Ginsburg D, Anlembom T, Wang J, Patel S, Li B, Hinnebusch A. NuA4 links methylation of histone H3 lysines 4 and 36 to acetylation of histones H4 and H3. J Biol Chem. 2014;289:32656-70 pubmed publisher
	Pan Y, Zhu Y, Yang W, Tycksen E, Liu S, Palucki J, et al. The role of Twist1 in mutant huntingtin-induced transcriptional alterations and neurotoxicity. J Biol Chem. 2018;293:11850-11866 pubmed publisher
	Schram A, Baas R, Jansen P, Riss A, Tora L, Vermeulen M, et al. A dual role for SAGA-associated factor 29 (SGF29) in ER stress survival by coordination of both histone H3 acetylation and histone H3 lysine-4 trimethylation. PLoS ONE. 2013;8:e70035 pubmed publisher
	Bugga L, McDaniel I, Engie L, Armstrong J. The Drosophila melanogaster CHD1 chromatin remodeling factor modulates global chromosome structure and counteracts HP1a and H3K9me2. PLoS ONE. 2013;8:e59496 pubmed publisher
	Lynch M, Smith A, De Gobbi M, Flenley M, Hughes J, Vernimmen D, et al. An interspecies analysis reveals a key role for unmethylated CpG dinucleotides in vertebrate Polycomb complex recruitment. EMBO J. 2012;31:317-29 pubmed publisher
	Vernimmen D, Lynch M, De Gobbi M, Garrick D, Sharpe J, Sloane Stanley J, et al. Polycomb eviction as a new distant enhancer function. Genes Dev. 2011;25:1583-8 pubmed publisher

product information

Catalog Number :

05-745R

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-trimethyl-Histone H3 (Lys4) Antibody, clone 15-10C-E4, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16695

Host Name :

Rabbit

Antigen :

trimethyl-Histone H3 (Lys4)

Clone :

15-10C-E4

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-trimethyl-Histone H3 (Lys4) Antibody, clone 15-10C-E4, rabbit monoclonal

Cross Reactivity :

Human

Background :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

ALT Names :

H3K4me3;Histone H3 (tri methyl K4)

Immunogen :

BSA-conjugated, synthetic peptide containing the sequence …RTme3KQT… in which me3K corresponds to trimethyl-lysine 4 of human histone H3.

Specificity :

Recognizes Histone H3 containing trimethyl-lysine 4 and, to a lesser extent, dimethyl-lysine 4. See certificate of analysis for further details.

Package Size :

100 μL

Uses :

Western Blotting;Chromatin Immunoprecipitation (ChIP);ChIP-seq;Dot Blot;ELISA;Peptide Inhibition Assay;Multiplexing

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.