trimethyl-Histone H3 (Lys4) Antibody, clone 15-10C-E4, Trial Size | EMD Millipore 05-745R-S product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

trimethyl-Histone H3 (Lys4) Antibody, clone 15-10C-E4, Trial Size

catalog :

05-745R-S

quantity :

10 μL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

15-10C-E4

reactivity :

human

application :

western blot, ELISA, chromatin immunoprecipitation, ChIP-Seq

citations: 3

Published Application/Species/Sample/Dilution	Reference
ChIP-Seq; human; loading ...; fig 3a	Kelso T, Porter D, Amaral M, Shokhirev M, Benner C, Hargreaves D. Chromatin accessibility underlies synthetic lethality of SWI/SNF subunits in ARID1A-mutant cancers. elife. 2017;6: pubmed publisher
	Pan Y, Zhu Y, Yang W, Tycksen E, Liu S, Palucki J, et al. The role of Twist1 in mutant huntingtin-induced transcriptional alterations and neurotoxicity. J Biol Chem. 2018;293:11850-11866 pubmed publisher
	Polier S, Samant R, Clarke P, Workman P, Prodromou C, Pearl L. ATP-competitive inhibitors block protein kinase recruitment to the Hsp90-Cdc37 system. Nat Chem Biol. 2013;9:307-12 pubmed publisher

product information

Catalog Number :

05-745R-S

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-trimethyl-Histone H3 (Lys4) Antibody, clone 15-10C-E4, Trial Size

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16695

Host Name :

Rabbit

Antigen :

H3F3A

Clone :

15-10C-E4

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-trimethyl-Histone H3 (Lys4) Antibody, clone 15-10C-E4, Trial Size

Cross Reactivity :

Human

Background :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

ALT Names :

H3K4me3, Histone H3 (tri methyl K4)

Immunogen :

BSA-conjugated, synthetic peptide containing the sequence …RTme3KQT… in which me3K corresponds to trimethyl-lysine 4 of human histone H3.

Package Size :

10 μL

Uses :

Western Blotting, Chromatin Immunoprecipitation (ChIP), ChIP-seq, Dot Blot, ELISA, Peptide Inhibition Assay, Multiplexing

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.