Bcl2 Antibody, clone 100 | EMD Millipore 05-729 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Bcl2 Antibody, clone 100

catalog :

05-729

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

100

The same clone is also sold as:

Santa Cruz Biotechnology: sc-509 AC, sc-509, sc-509 HRP
BioLegend: 658709, 658706, 658707, 658703, 658708, 658702, 658701, 658705, 658704
Invitrogen: MHBCL01, MHBCL04
Bio-Rad: MCA1550, VMA00017

reactivity :

human, dogs

application :

western blot, immunohistochemistry, flow cytometry

citations: 14

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 6	Matias A, Manieri T, Cerchiaro G. Zinc Chelation Mediates the Lysosomal Disruption without Intracellular ROS Generation. Oxid Med Cell Longev. 2016;2016:6724585 pubmed publisher
western blot; human; tbl s3	Cheng H, Liang Y, Kuo Y, Chuu C, Lin C, Lee M, et al. Identification of thioridazine, an antipsychotic drug, as an antiglioblastoma and anticancer stem cell agent using public gene expression data. Cell Death Dis. 2015;6:e1753 pubmed publisher
western blot; human	Rayavarapu R, Heiden B, Pagani N, Shaw M, Shuff S, Zhang S, et al. The role of multicellular aggregation in the survival of ErbB2-positive breast cancer cells during extracellular matrix detachment. J Biol Chem. 2015;290:8722-33 pubmed publisher
western blot; human	Guerzoni C, Amatori S, Giorgi L, Manara M, Landuzzi L, Lollini P, et al. An aza-macrocycle containing maltolic side-arms (maltonis) as potential drug against human pediatric sarcomas. BMC Cancer. 2014;14:137 pubmed publisher
	Coni S, Mancuso A, Di Magno L, Sdruscia G, Manni S, Serrao S, et al. Selective targeting of HDAC1/2 elicits anticancer effects through Gli1 acetylation in preclinical models of SHH Medulloblastoma. Sci Rep. 2017;7:44079 pubmed publisher
	Keilhoff G, Lucas B, Uhde K, Fansa H. Selected gene profiles of stressed NSC-34 cells and rat spinal cord following peripheral nerve reconstruction and minocycline treatment. Exp Ther Med. 2016;11:1685-1699 pubmed
	Huang H, Chen X, Li D, He Y, Li Y, Du Z, et al. Combination of Î±-Tomatine and Curcumin Inhibits Growth and Induces Apoptosis in Human Prostate Cancer Cells. PLoS ONE. 2015;10:e0144293 pubmed publisher
	Dhayat S, Mardin W, SeggewiÃŸ J, StrÃ¶se A, Matuszcak C, Hummel R, et al. MicroRNA Profiling Implies New Markers of Gemcitabine Chemoresistance in Mutant p53 Pancreatic Ductal Adenocarcinoma. PLoS ONE. 2015;10:e0143755 pubmed publisher
	Lizarraga F, Ceballos Cancino G, Espinosa M, Vazquez Santillan K, Maldonado V, Melendez Zajgla J. Tissue Inhibitor of Metalloproteinase-4 Triggers Apoptosis in Cervical Cancer Cells. PLoS ONE. 2015;10:e0135929 pubmed publisher
	Huang H, Cui X, Chen S, Goodin S, Liu Y, He Y, et al. Combination of Lipitor and Celebrex inhibits prostate cancer VCaP cells in vitro and in vivo. Anticancer Res. 2014;34:3357-63 pubmed
	Prescimone T, Masotti S, D Amico A, Caruso R, Cabiati M, Caselli C, et al. Cardiac molecular markers of programmed cell death are activated in end-stage heart failure patients supported by left ventricular assist device. Cardiovasc Pathol. 2014;23:272-82 pubmed publisher
	EiÃŸmann M, Schwamb B, Melzer I, Moser J, Siele D, KÃ¶hl U, et al. A functional yeast survival screen of tumor-derived cDNA libraries designed to identify anti-apoptotic mammalian oncogenes. PLoS ONE. 2013;8:e64873 pubmed publisher
	Chiang K, Yeh C, Chen S, Shen S, Hsu J, Yeh T, et al. MART-10, a New Generation of Vitamin D Analog, Is More Potent than 1?,25-Dihydroxyvitamin D(3) in Inhibiting Cell Proliferation and Inducing Apoptosis in ER+ MCF-7 Breast Cancer Cells. Evid Based Complement Alternat Med. 2012;2012:310872 pubmed publisher
	Criollo A, Galluzzi L, Maiuri M, Tasdemir E, Lavandero S, Kroemer G. Mitochondrial control of cell death induced by hyperosmotic stress. Apoptosis. 2007;12:3-18 pubmed

product information

Catalog Number :

05-729

Subcategory :

Apoptosis & Cancer

Product Name :

Anti-Bcl2 Antibody, clone 100

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P10415

Host Name :

Mouse

Antigen :

Bcl2

Clone :

100

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Bcl2 Antibody, clone 100

Cross Reactivity :

Human;Canine;Feline

Background :

The Bcl name is derived from B-cell chronic lymphocytic leukemia, and was given to genes involved in that disease. Members of the Bcl2 family share homologous domains (BH) through which they are able to homo- and hetero-dimerize. Apoptosis/survival is regulated by the relative balance of pro-apoptotic (Bax, BclXS, Bak, Bad) and anti-apoptotic (Bcl2, BclXL, Mcl-1, Dad-1) family members, and their heterodimerization. Bcl2 plays an important role in cytochrome c release from the mitochondria; it also binds Apaf1, inhibiting Apaf-1 association with caspase-9. Bcl-2 overexpression in indicated in a number of human cancers, including melanoma, breast, prostate, and lung, and it correlates with resistance to cancer treatment (presumably due to decreased apoptosis). Bcl-2 has also been implicated in schizophrenia and autoimmune disorders.

ALT Names :

B-cell CLL/lymphoma 2;B-cell lymphoma protein 2

Immunogen :

Synthetic peptide corresponding to amino acids 41-54 of human Bcl2 protein. The immunizing sequence is identical in canine and feline.

Specificity :

This antibody recognizes Bcl2, Mr 26 kDa.

Package Size :

100 µg

Uses :

Flow Cytometry;Immunohistochemistry;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.