This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Myc Tag Antibody, clone 4A6
catalog :
05-724
quantity :
200 μg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4A6
reactivity :
Toxoplasma gondii, human, rat
application :
western blot, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation
citations: 80
Published Application/Species/Sample/DilutionReference
  • western blot; Toxoplasma gondii; loading ...; fig 7d
Broncel M, Dominicus C, Vigetti L, Nofal S, Bartlett E, Touquet B, et al. Profiling of myristoylation in Toxoplasma gondii reveals an N-myristoylated protein important for host cell penetration. elife. 2020;9: pubmed publisher
  • western blot; human; 1:2000; loading ...
Kido K, Yamanaka S, Nakano S, Motani K, Shinohara S, Nozawa A, et al. AirID, a novel proximity biotinylation enzyme, for analysis of protein-protein interactions. elife. 2020;9: pubmed publisher
  • immunoprecipitation; human; fig 5a
Li N, Truong S, Nouri M, Moore J, Al Nakouzi N, Lubik A, et al. Non-canonical activation of hedgehog in prostate cancer cells mediated by the interaction of transcriptionally active androgen receptor proteins with Gli3. Oncogene. 2018;37:2313-2325 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 2a
Allison R, Edgar J, Pearson G, Rizo T, Newton T, Günther S, et al. Defects in ER-endosome contacts impact lysosome function in hereditary spastic paraplegia. J Cell Biol. 2017;216:1337-1355 pubmed publisher
  • western blot; human; 1:1000; fig s23
Karginov A, Tsygankov D, Berginski M, Chu P, Trudeau E, Yi J, et al. Dissecting motility signaling through activation of specific Src-effector complexes. Nat Chem Biol. 2014;10:286-90 pubmed publisher
Bittmann J, Grigaitis R, Galanti L, Amarell S, Wilfling F, Matos J, et al. An advanced cell cycle tag toolbox reveals principles underlying temporal control of structure-selective nucleases. elife. 2020;9: pubmed publisher
Tai Y, Gallo N, Wang M, Yu J, Van Aelst L. Axo-axonic Innervation of Neocortical Pyramidal Neurons by GABAergic Chandelier Cells Requires AnkyrinG-Associated L1CAM. Neuron. 2019;102:358-372.e9 pubmed publisher
Hunter M, Hesketh G, Benedyk T, Gingras A, Graham S. Proteomic and Biochemical Comparison of the Cellular Interaction Partners of Human VPS33A and VPS33B. J Mol Biol. 2018;430:2153-2163 pubmed publisher
Cieri D, Vicario M, Giacomello M, Vallese F, Filadi R, Wagner T, et al. SPLICS: a split green fluorescent protein-based contact site sensor for narrow and wide heterotypic organelle juxtaposition. Cell Death Differ. 2018;25:1131-1145 pubmed publisher
Huang J, Ku W, Chen Y, Chang Y, Chu C. Dual mechanisms regulate the nucleocytoplasmic localization of human DDX6. Sci Rep. 2017;7:42853 pubmed publisher
Moreira B, Fonseca C, Hammarton T, Baqui M. Giant FAZ10 is required for flagellum attachment zone stabilization and furrow positioning in Trypanosoma brucei. J Cell Sci. 2017;130:1179-1193 pubmed publisher
Young C, Hillyer C, Hokamp K, Fitzpatrick D, Konstantinov N, Welty J, et al. Distinct histone methylation and transcription profiles are established during the development of cellular quiescence in yeast. BMC Genomics. 2017;18:107 pubmed publisher
Mauricio R, Jeffries C, Svergun D, Deane J. The Shigella Virulence Factor IcsA Relieves N-WASP Autoinhibition by Displacing the Verprolin Homology/Cofilin/Acidic (VCA) Domain. J Biol Chem. 2017;292:134-145 pubmed publisher
Kaufmann T, Kukolj E, Brachner A, Beltzung E, Bruno M, Kostrhon S, et al. SIRT2 regulates nuclear envelope reassembly through ANKLE2 deacetylation. J Cell Sci. 2016;129:4607-4621 pubmed
Gu P, Wang Y, Bisht K, Wu L, Kukova L, Smith E, et al. Pot1 OB-fold mutations unleash telomere instability to initiate tumorigenesis. Oncogene. 2017;36:1939-1951 pubmed publisher
Spencer Smith R, Koide A, Zhou Y, Eguchi R, Sha F, Gajwani P, et al. Inhibition of RAS function through targeting an allosteric regulatory site. Nat Chem Biol. 2017;13:62-68 pubmed publisher
Belagal P, Normand C, Shukla A, Wang R, Léger Silvestre I, Dez C, et al. Decoding the principles underlying the frequency of association with nucleoli for RNA polymerase III-transcribed genes in budding yeast. Mol Biol Cell. 2016;27:3164-3177 pubmed
Hamp J, Löwer A, Dottermusch Heidel C, Beck L, Moussian B, Flötenmeyer M, et al. Drosophila Kette coordinates myoblast junction dissolution and the ratio of Scar-to-WASp during myoblast fusion. J Cell Sci. 2016;129:3426-36 pubmed publisher
Theodosiou M, Widmaier M, Böttcher R, Rognoni E, Veelders M, Bharadwaj M, et al. Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin. elife. 2016;5:e10130 pubmed publisher
Chavoshi S, Egorova O, Lacdao I, Farhadi S, Sheng Y, Saridakis V. Identification of Kaposi Sarcoma Herpesvirus (KSHV) vIRF1 Protein as a Novel Interaction Partner of Human Deubiquitinase USP7. J Biol Chem. 2016;291:6281-91 pubmed publisher
Wang J, Lu R, Yang J, Li H, He Z, Jing N, et al. TRPC6 specifically interacts with APP to inhibit its cleavage by γ-secretase and reduce Aβ production. Nat Commun. 2015;6:8876 pubmed publisher
Woods N, Trevino J, Coppola D, Chellappan S, Yang S, Padmanabhan J. Fendiline inhibits proliferation and invasion of pancreatic cancer cells by interfering with ADAM10 activation and β-catenin signaling. Oncotarget. 2015;6:35931-48 pubmed publisher
Okere B, Alviano F, Costa R, Quaglino D, Ricci F, Dominici M, et al. In vitro differentiation of human amniotic epithelial cells into insulin-producing 3D spheroids. Int J Immunopathol Pharmacol. 2015;28:390-402 pubmed publisher
Schroeder G, Aurass P, Oates C, Tate E, Hartland E, Flieger A, et al. Legionella pneumophila Effector LpdA Is a Palmitoylated Phospholipase D Virulence Factor. Infect Immun. 2015;83:3989-4002 pubmed publisher
Gurden M, Westwood I, Faisal A, Naud S, Cheung K, McAndrew C, et al. Naturally Occurring Mutations in the MPS1 Gene Predispose Cells to Kinase Inhibitor Drug Resistance. Cancer Res. 2015;75:3340-54 pubmed publisher
Nyathi Y, Pool M. Analysis of the interplay of protein biogenesis factors at the ribosome exit site reveals new role for NAC. J Cell Biol. 2015;210:287-301 pubmed publisher
Pentecost M, Vashisht A, Lester T, Voros T, Beaty S, Park A, et al. Evidence for ubiquitin-regulated nuclear and subnuclear trafficking among Paramyxovirinae matrix proteins. PLoS Pathog. 2015;11:e1004739 pubmed publisher
Koob S, Barrera M, Anand R, Reichert A. The non-glycosylated isoform of MIC26 is a constituent of the mammalian MICOS complex and promotes formation of crista junctions. Biochim Biophys Acta. 2015;1853:1551-63 pubmed publisher
Kaehler C, Guenther A, Uhlich A, Krobitsch S. PRMT1-mediated arginine methylation controls ATXN2L localization. Exp Cell Res. 2015;334:114-25 pubmed publisher
Zhang S, Qi J, Li X, Wang H, Britt J, Hoffman A, et al. Dopaminergic and glutamatergic microdomains in a subset of rodent mesoaccumbens axons. Nat Neurosci. 2015;18:386-92 pubmed publisher
Sekimoto T, Oda T, Kurashima K, Hanaoka F, Yamashita T. Both high-fidelity replicative and low-fidelity Y-family polymerases are involved in DNA rereplication. Mol Cell Biol. 2015;35:699-715 pubmed publisher
Kong X, Ma S, Guo J, Ma Y, Hu Y, Wang J, et al. Ubiquitously expressed transcript is a novel interacting protein of protein inhibitor of activated signal transducer and activator of transcription 2. Mol Med Rep. 2015;11:2443-8 pubmed publisher
Kwon Y, Song P, Yoon J, Ghim J, Kim D, Kang B, et al. Xanthene derivatives increase glucose utilization through activation of LKB1-dependent AMP-activated protein kinase. PLoS ONE. 2014;9:e108771 pubmed publisher
Polakowski N, Terol M, Hoang K, Nash I, Laverdure S, Gazon H, et al. HBZ stimulates brain-derived neurotrophic factor/TrkB autocrine/paracrine signaling to promote survival of human T-cell leukemia virus type 1-Infected T cells. J Virol. 2014;88:13482-94 pubmed publisher
Pallett M, Berger C, Pearson J, Hartland E, Frankel G. The type III secretion effector NleF of enteropathogenic Escherichia coli activates NF-κB early during infection. Infect Immun. 2014;82:4878-88 pubmed publisher
Mousnier A, Schroeder G, Stoneham C, So E, Garnett J, Yu L, et al. A new method to determine in vivo interactomes reveals binding of the Legionella pneumophila effector PieE to multiple rab GTPases. MBio. 2014;5: pubmed publisher
Fleming A, Beggs S, CHURCH M, Tsukihashi Y, Pennings S. The yeast Cyc8-Tup1 complex cooperates with Hda1p and Rpd3p histone deacetylases to robustly repress transcription of the subtelomeric FLO1 gene. Biochim Biophys Acta. 2014;1839:1242-55 pubmed publisher
Mousnier A, Swieboda D, Pinto A, Guedán A, Rogers A, Walton R, et al. Human rhinovirus 16 causes Golgi apparatus fragmentation without blocking protein secretion. J Virol. 2014;88:11671-85 pubmed publisher
Cao Y, Rathmell J, Macintyre A. Metabolic reprogramming towards aerobic glycolysis correlates with greater proliferative ability and resistance to metabolic inhibition in CD8 versus CD4 T cells. PLoS ONE. 2014;9:e104104 pubmed publisher
Takenaka N, Yasuda N, Nihata Y, Hosooka T, Noguchi T, Aiba A, et al. Role of the guanine nucleotide exchange factor in Akt2-mediated plasma membrane translocation of GLUT4 in insulin-stimulated skeletal muscle. Cell Signal. 2014;26:2460-9 pubmed publisher
Uhlenbrock F, Hagemann Jensen M, Kehlet S, Andresen L, Pastorekova S, Skov S. The NKG2D ligand ULBP2 is specifically regulated through an invariant chain-dependent endosomal pathway. J Immunol. 2014;193:1654-65 pubmed publisher
Kobayashi H, Saito T, Sato K, Furusawa K, Hosokawa T, Tsutsumi K, et al. Phosphorylation of cyclin-dependent kinase 5 (Cdk5) at Tyr-15 is inhibited by Cdk5 activators and does not contribute to the activation of Cdk5. J Biol Chem. 2014;289:19627-36 pubmed publisher
Mellergaard M, Skovbakke S, Schneider C, Lauridsen F, Andresen L, Jensen H, et al. N-glycosylation of asparagine 8 regulates surface expression of major histocompatibility complex class I chain-related protein A (MICA) alleles dependent on threonine 24. J Biol Chem. 2014;289:20078-91 pubmed publisher
Bersten D, Bruning J, Peet D, Whitelaw M. Human variants in the neuronal basic helix-loop-helix/Per-Arnt-Sim (bHLH/PAS) transcription factor complex NPAS4/ARNT2 disrupt function. PLoS ONE. 2014;9:e85768 pubmed publisher
Zhou X, Han X, Zhao K, Du J, Evans S, Wang H, et al. Dispersed and conserved hydrophobic residues of HIV-1 Vif are essential for CBF? recruitment and A3G suppression. J Virol. 2014;88:2555-63 pubmed publisher
Gualeni B, Rajpar M, Kellogg A, Bell P, Arvan P, Boot Handford R, et al. A novel transgenic mouse model of growth plate dysplasia reveals that decreased chondrocyte proliferation due to chronic ER stress is a key factor in reduced bone growth. Dis Model Mech. 2013;6:1414-25 pubmed publisher
Woods N, Padmanabhan J. Inhibition of amyloid precursor protein processing enhances gemcitabine-mediated cytotoxicity in pancreatic cancer cells. J Biol Chem. 2013;288:30114-24 pubmed publisher
Robinson J, Pitcher J. G protein-coupled receptor kinase 2 (GRK2) is a Rho-activated scaffold protein for the ERK MAP kinase cascade. Cell Signal. 2013;25:2831-9 pubmed publisher
Harding C, Mattheis C, Mousnier A, Oates C, Hartland E, Frankel G, et al. LtpD is a novel Legionella pneumophila effector that binds phosphatidylinositol 3-phosphate and inositol monophosphatase IMPA1. Infect Immun. 2013;81:4261-70 pubmed publisher
Regla Nava J, Jiménez Guardeño J, Nieto Torres J, Gallagher T, Enjuanes L, DeDiego M. The replication of a mouse adapted SARS-CoV in a mouse cell line stably expressing the murine SARS-CoV receptor mACE2 efficiently induces the expression of proinflammatory cytokines. J Virol Methods. 2013;193:639-46 pubmed publisher
Borowiak M, Kuhlmann A, Girard S, Gazzolo L, Mesnard J, Jalinot P, et al. HTLV-1 bZIP factor impedes the menin tumor suppressor and upregulates JunD-mediated transcription of the hTERT gene. Carcinogenesis. 2013;34:2664-72 pubmed publisher
Viswanathan J, Haapasalo A, Kurkinen K, Natunen T, Mäkinen P, Bertram L, et al. Ubiquilin-1 modulates ?-secretase-mediated ?-site cleavage in neuronal cells. Biochemistry. 2013;52:3899-912 pubmed publisher
Sarkari F, Wheaton K, La Delfa A, Mohamed M, Shaikh F, Khatun R, et al. Ubiquitin-specific protease 7 is a regulator of ubiquitin-conjugating enzyme UbE2E1. J Biol Chem. 2013;288:16975-85 pubmed publisher
Paster W, Brockmeyer C, Fu G, Simister P, de Wet B, Martinez Riaño A, et al. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. J Immunol. 2013;190:3749-56 pubmed publisher
Wirth M, Karaca S, Wenzel D, Ho L, Tishkoff D, Lombard D, et al. Mitochondrial SIRT4-type proteins in Caenorhabditis elegans and mammals interact with pyruvate carboxylase and other acetylated biotin-dependent carboxylases. Mitochondrion. 2013;13:705-20 pubmed publisher
Mussche S, Devreese B, Nagabhushan Kalburgi S, Bachaboina L, Fox J, Shih H, et al. Restoration of cytoskeleton homeostasis after gigaxonin gene transfer for giant axonal neuropathy. Hum Gene Ther. 2013;24:209-19 pubmed publisher
Wang H, Zhang Z, Li H, Zhao X, Liu X, Ortiz M, et al. CONSTANS-LIKE 7 regulates branching and shade avoidance response in Arabidopsis. J Exp Bot. 2013;64:1017-24 pubmed publisher
Trickey M, Fujimitsu K, Yamano H. Anaphase-promoting complex/cyclosome-mediated proteolysis of Ams2 in the G1 phase ensures the coupling of histone gene expression to DNA replication in fission yeast. J Biol Chem. 2013;288:928-37 pubmed publisher
Li Y, Xiao H, Chiou T, Jin H, Bonhomme B, Miralles C, et al. Molecular and functional interaction between protocadherin-?C5 and GABAA receptors. J Neurosci. 2012;32:11780-97 pubmed publisher
Kung L, Rajpar M, Briggs M, Boot Handford R. Hypertrophic chondrocytes have a limited capacity to cope with increases in endoplasmic reticulum stress without triggering the unfolded protein response. J Histochem Cytochem. 2012;60:734-48 pubmed
Zheng Y, Tan X, Pyczek J, Nolte J, Pantakani D, Engel W. Generation and characterization of yeast two-hybrid cDNA libraries derived from two distinct mouse pluripotent cell types. Mol Biotechnol. 2013;54:228-37 pubmed publisher
Wurm T, Wright D, Polakowski N, Mesnard J, Lemasson I. The HTLV-1-encoded protein HBZ directly inhibits the acetyl transferase activity of p300/CBP. Nucleic Acids Res. 2012;40:5910-25 pubmed publisher
Urbé S, Liu H, Hayes S, Heride C, Rigden D, Clague M. Systematic survey of deubiquitinase localization identifies USP21 as a regulator of centrosome- and microtubule-associated functions. Mol Biol Cell. 2012;23:1095-103 pubmed publisher
Sharma S, Gerke D, Han H, Jeong S, Stallcup M, Jones P, et al. Lysine methyltransferase G9a is not required for DNMT3A/3B anchoring to methylated nucleosomes and maintenance of DNA methylation in somatic cells. Epigenetics Chromatin. 2012;5:3 pubmed publisher
Li J, Hu P, Li K, Zhao Q. Identification and characterization of a novel retinoic acid response element in zebrafish cyp26a1 promoter. Anat Rec (Hoboken). 2012;295:268-77 pubmed publisher
Needham P, Mikoluk K, Dhakarwal P, Khadem S, Snyder A, Subramanya A, et al. The thiazide-sensitive NaCl cotransporter is targeted for chaperone-dependent endoplasmic reticulum-associated degradation. J Biol Chem. 2011;286:43611-21 pubmed publisher
Dobson M, Diallo Krou E, Grachtchouk V, Yu J, Colby L, Wilkinson J, et al. Pioglitazone induces a proadipogenic antitumor response in mice with PAX8-PPARgamma fusion protein thyroid carcinoma. Endocrinology. 2011;152:4455-65 pubmed publisher
Froyd C, Rusche L. The duplicated deacetylases Sir2 and Hst1 subfunctionalized by acquiring complementary inactivating mutations. Mol Cell Biol. 2011;31:3351-65 pubmed publisher
Prescott E, Safi A, Rusche L. A region of the nucleosome required for multiple types of transcriptional silencing in Saccharomyces cerevisiae. Genetics. 2011;188:535-48 pubmed publisher
Chiou T, Bonhomme B, Jin H, Miralles C, Xiao H, Fu Z, et al. Differential regulation of the postsynaptic clustering of γ-aminobutyric acid type A (GABAA) receptors by collybistin isoforms. J Biol Chem. 2011;286:22456-68 pubmed publisher
Chang C, Lai L, Cheng H, Chen K, Syue Y, Lu H, et al. TBK1-associated protein in endolysosomes (TAPE) is an innate immune regulator modulating the TLR3 and TLR4 signaling pathways. J Biol Chem. 2011;286:7043-51 pubmed publisher
Conde F, Ontoso D, Acosta I, Gallego Sánchez A, Bueno A, San Segundo P. Regulation of tolerance to DNA alkylating damage by Dot1 and Rad53 in Saccharomyces cerevisiae. DNA Repair (Amst). 2010;9:1038-49 pubmed publisher
Polakowski N, Gregory H, Mesnard J, Lemasson I. Expression of a protein involved in bone resorption, Dkk1, is activated by HTLV-1 bZIP factor through its activation domain. Retrovirology. 2010;7:61 pubmed publisher
Doyle K, Fitzpatrick F. Redox signaling, alkylation (carbonylation) of conserved cysteines inactivates class I histone deacetylases 1, 2, and 3 and antagonizes their transcriptional repressor function. J Biol Chem. 2010;285:17417-24 pubmed publisher
Enomoto M, Goto H, Tomono Y, Kasahara K, Tsujimura K, Kiyono T, et al. Novel positive feedback loop between Cdk1 and Chk1 in the nucleus during G2/M transition. J Biol Chem. 2009;284:34223-30 pubmed publisher
Fan J, Cowan C, Zhang L, Hayden M, Raymond L. Interaction of postsynaptic density protein-95 with NMDA receptors influences excitotoxicity in the yeast artificial chromosome mouse model of Huntington's disease. J Neurosci. 2009;29:10928-38 pubmed publisher
Fujiyama Nakamura S, Ito S, Sawatsubashi S, Yamauchi Y, Suzuki E, Tanabe M, et al. BTB protein, dKLHL18/CG3571, serves as an adaptor subunit for a dCul3 ubiquitin ligase complex. Genes Cells. 2009;14:965-73 pubmed publisher
Jeong S, Liang G, Sharma S, Lin J, Choi S, Han H, et al. Selective anchoring of DNA methyltransferases 3A and 3B to nucleosomes containing methylated DNA. Mol Cell Biol. 2009;29:5366-76 pubmed publisher
Safi A, Wallace K, Rusche L. Evolution of new function through a single amino acid change in the yeast repressor Sum1p. Mol Cell Biol. 2008;28:2567-78 pubmed publisher
Fan S, Fogg V, Wang Q, Chen X, Liu C, Margolis B. A novel Crumbs3 isoform regulates cell division and ciliogenesis via importin beta interactions. J Cell Biol. 2007;178:387-98 pubmed
product information
Catalog Number :
05-724
Subcategory :
Epitope Tags & General Use
Product Name :
Anti-Myc Tag Antibody, clone 4A6
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P01106
Host Name :
Mouse
Antigen :
Myc Tag
Clone :
4A6
Conjugate :
Purified
Isotype :
IgG
Product Description :
Anti-Myc Tag Antibody, clone 4A6
Cross Reactivity :
Human
Background :
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.
Immunogen :
peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc
Specificity :
Recognizes recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts; also recognizes human Myc.
Package Size :
200 μg
Uses :
Immunocytochemistry;Immunofluorescence;Immunoprecipitation;Western Blotting;ChIP-seq
Storage :
Maintain at 2 to 8°C for 1 year from date of shipment.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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