RhoE/Rnd3 Antibody, clone 4 | EMD Millipore 05-723 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

RhoE/Rnd3 Antibody, clone 4

catalog :

05-723

quantity :

200 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4

The same clone is also sold as:

Santa Cruz Biotechnology: sc-53874
Cell Signaling Technology: 3664

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry

citations: 7

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:100; fig 1 western blot; human; 1:1000; fig 2	Breslin J, Daines D, Doggett T, Kurtz K, Souza Smith F, Zhang X, et al. Rnd3 as a Novel Target to Ameliorate Microvascular Leakage. J Am Heart Assoc. 2016;5:e003336 pubmed publisher
immunocytochemistry; mouse; 1:100	Obeidat M, Li L, Ballermann B. TIMAP promotes angiogenesis by suppressing PTEN-mediated Akt inhibition in human glomerular endothelial cells. Am J Physiol Renal Physiol. 2014;307:F623-33 pubmed publisher
immunohistochemistry; human; 1:50 western blot; human	Zhu Y, Zhou J, Xia H, Chen X, Qiu M, Huang J, et al. The Rho GTPase RhoE is a p53-regulated candidate tumor suppressor in cancer cells. Int J Oncol. 2014;44:896-904 pubmed publisher
	Frank S, Köllmann C, Luong P, Galli G, Zou L, Bernards A, et al. p190 RhoGAP promotes contact inhibition in epithelial cells by repressing YAP activity. J Cell Biol. 2018;217:3183-3201 pubmed publisher
	Riz I, Hawley T, Marsal J, Hawley R. Noncanonical SQSTM1/p62-Nrf2 pathway activation mediates proteasome inhibitor resistance in multiple myeloma cells via redox, metabolic and translational reprogramming. Oncotarget. 2016;7:66360-66385 pubmed publisher
	Thuault S, Comunale F, Hasna J, Fortier M, Planchon D, Elarouci N, et al. The RhoE/ROCK/ARHGAP25 signaling pathway controls cell invasion by inhibition of Rac activity. Mol Biol Cell. 2016;27:2653-61 pubmed publisher
	Klein R, Higgins P. A switch in RND3-RHOA signaling is critical for melanoma cell invasion following mutant-BRAF inhibition. Mol Cancer. 2011;10:114 pubmed publisher

product information

Catalog Number :

05-723

Subcategory :

Cell Signaling

Product Name :

Anti-RhoE/Rnd3 Antibody, clone 4

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P61587

Host Name :

Mouse

Antigen :

RhoE/Rnd3

Clone :

Conjugate :

Ascites

Isotype :

IgG

Product Description :

Anti-RhoE/Rnd3 Antibody, clone 4

Cross Reactivity :

Human;Mouse

Background :

RhoE, also known as Rnd3, (Rho family GTPase 3) is a small G-protein that is a member of the Rnd family of Rho GTPases, which is a part of the Ras superfamily. The activity of Rnd1, Rnd2, and Rnd3/RhoE are not activated by the traditional conformational change of the GTP binding like other Rho family members. As the name dictates, Rnd proteins were associated with making a cell “round”. Like other small G-proteins, the do bind GTP, but unlike other G-proteins, they do not hydrolyze GTP to GDP. The actions of RhoE are associated with the actin cytoskeletal organization in response to external stimuli. RhoE acts antagonistically to RhoA in inducing loss of stress fiber formation, in part by binding to the RhoA downstream kinase Rho-associated kinase (ROCK1). This prevents ROCK1 activation and inhibits downstream signaling. RhoE does go through the post-translation modification of farnylation which enables it to localize and bind to various internal membranes. RhoE is also known to effect cycle progression.

ALT Names :

MemB protein;Rho family GTPase 3;ras homolog gene family, member E;small GTP binding protein Rho8

Immunogen :

Full length fusion protein corresponding to residues 1-244 of mouse RhoE/Rnd3. Clone 4.

Specificity :

This antibody recognizes RhoE/Rnd3, Mr 29 kDa. Weakly cross-reacts with Rnd1, Mr 27 kDa.

Package Size :

200 µL

Uses :

Immunocytochemistry;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.