Adenosine Receptor A2a Antibody, clone 7F6-G5-A2 | EMD Millipore 05-717 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Adenosine Receptor A2a Antibody, clone 7F6-G5-A2

catalog :

05-717

quantity :

200 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7F6-G5-A2

The same clone is also sold as:

Novus Biologicals: NBP2-80562, NBP1-39474
Santa Cruz Biotechnology: sc-32261
Abcam: ab79714

reactivity :

African green monkey, human, mouse, rat, dogs

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section, western blot knockout validation, immunohistochemistry knockout validation

citations: 21

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry knockout validation; mouse; 1:10,000; fig s3 immunohistochemistry - frozen section; mouse; 1:200; fig 7 western blot; mouse; 1:500; fig s2 immunohistochemistry - frozen section; human; 1:50; fig s1 western blot; human; 1:500; fig s2	Orr A, Hsiao E, Wang M, Ho K, Kim D, Wang X, et al. Astrocytic adenosine receptor A2A and Gs-coupled signaling regulate memory. Nat Neurosci. 2015;18:423-34 pubmed publisher
western blot knockout validation; mouse; 1:500; fig 4	Matos M, Augusto E, Santos Rodrigues A, Schwarzschild M, Chen J, Cunha R, et al. Adenosine A2A receptors modulate glutamate uptake in cultured astrocytes and gliosomes. Glia. 2012;60:702-16 pubmed publisher
immunocytochemistry; rat; loading ...; fig 6a	Guinzberg R, Díaz Cruz A, Acosta Trujillo C, Vilchis Landeros M, Vázquez Meza H, Lozano Flores C, et al. Newly synthesized cAMP is integrated at a membrane protein complex signalosome to ensure receptor response specificity. FEBS J. 2017;284:258-276 pubmed publisher
western blot; rat; 1:2000; fig 1	Batalha V, Ferreira D, Coelho J, Valadas J, Gomes R, Temido Ferreira M, et al. The caffeine-binding adenosine A2A receptor induces age-like HPA-axis dysfunction by targeting glucocorticoid receptor function. Sci Rep. 2016;6:31493 pubmed publisher
immunocytochemistry; mouse; 1:1000	Biezonski D, Trifilieff P, Meszaros J, Javitch J, Kellendonk C. Evidence for limited D1 and D2 receptor coexpression and colocalization within the dorsal striatum of the neonatal mouse. J Comp Neurol. 2015;523:1175-89 pubmed publisher
immunocytochemistry; human; 1:1000	Delli Carri A, Onorati M, Castiglioni V, Faedo A, Camnasio S, Toselli M, et al. Human pluripotent stem cell differentiation into authentic striatal projection neurons. Stem Cell Rev. 2013;9:461-74 pubmed publisher
flow cytometry; human immunocytochemistry; human western blot; human	FernÃ¡ndez DueÃ±as V, GÃ³mez Soler M, MoratÃ³ X, NÃºÃ±ez F, Das A, Kumar T, et al. Dopamine D(2) receptor-mediated modulation of adenosine A(2A) receptor agonist binding within the A(2A)R/D(2)R oligomer framework. Neurochem Int. 2013;63:42-6 pubmed publisher
western blot; human; 1:2000 western blot; African green monkey; 1:2000 western blot; rat; 1:2000	Bogenpohl J, Ritter S, Hall R, Smith Y. Adenosine A2A receptor in the monkey basal ganglia: ultrastructural localization and colocalization with the metabotropic glutamate receptor 5 in the striatum. J Comp Neurol. 2012;520:570-89 pubmed publisher
	Navarro G, Cordomi A, Brugarolas M, Moreno E, Aguinaga D, Pérez Benito L, et al. Cross-communication between Gi and Gs in a G-protein-coupled receptor heterotetramer guided by a receptor C-terminal domain. BMC Biol. 2018;16:24 pubmed publisher
	Sokolina K, Kittanakom S, Snider J, Kotlyar M, Maurice P, Gandia J, et al. Systematic protein-protein interaction mapping for clinically relevant human GPCRs. Mol Syst Biol. 2017;13:918 pubmed publisher
	Lu J, Cui J, Li X, Wang X, Zhou Y, Yang W, et al. An Anti-Parkinson's Disease Drug via Targeting Adenosine A2A Receptor Enhances Amyloid-? Generation and ?-Secretase Activity. PLoS ONE. 2016;11:e0166415 pubmed publisher
	Oliveira L, Correia A, Cristina Costa A, Guerra Gomes S, Ferreirinha F, MagalhÃ£es Cardoso M, et al. Deficits in endogenous adenosine formation by ecto-5'-nucleotidase/CD73 impair neuromuscular transmission and immune competence in experimental autoimmune myasthenia gravis. Mediators Inflamm. 2015;2015:460610 pubmed publisher
	Huang P, Hsiao Y, Kao S, Chen C, Chen Y, Chiang C, et al. Adenosine A(2A) receptor up-regulates retinal wave frequency via starburst amacrine cells in the developing rat retina. PLoS ONE. 2014;9:e95090 pubmed publisher
	Gyoneva S, Shapiro L, Lazo C, Garnier Amblard E, Smith Y, Miller G, et al. Adenosine A2A receptor antagonism reverses inflammation-induced impairment of microglial process extension in a model of Parkinson's disease. Neurobiol Dis. 2014;67:191-202 pubmed publisher
	Thurner P, Gsandtner I, Kudlacek O, Choquet D, Nanoff C, Freissmuth M, et al. A two-state model for the diffusion of the A2A adenosine receptor in hippocampal neurons: agonist-induced switch to slow mobility is modified by synapse-associated protein 102 (SAP102). J Biol Chem. 2014;289:9263-74 pubmed publisher
	Bonaventura J, Rico A, Moreno E, Sierra S, Sanchez M, Luquin N, et al. L-DOPA-treatment in primates disrupts the expression of A(2A) adenosine-CB(1) cannabinoid-D(2) dopamine receptor heteromers in the caudate nucleus. Neuropharmacology. 2014;79:90-100 pubmed publisher
	Valadas J, Batalha V, Ferreira D, Gomes R, Coelho J, Sebastião A, et al. Neuroprotection afforded by adenosine A2A receptor blockade is modulated by corticotrophin-releasing factor (CRF) in glutamate injured cortical neurons. J Neurochem. 2012;123:1030-40 pubmed publisher
	Batalha V, Pego J, Fontinha B, Costenla A, Valadas J, Baqi Y, et al. Adenosine A(2A) receptor blockade reverts hippocampal stress-induced deficits and restores corticosterone circadian oscillation. Mol Psychiatry. 2013;18:320-31 pubmed publisher
	Trifilieff P, Rives M, Urizar E, Piskorowski R, Vishwasrao H, Castrillon J, et al. Detection of antigen interactions ex vivo by proximity ligation assay: endogenous dopamine D2-adenosine A2A receptor complexes in the striatum. Biotechniques. 2011;51:111-8 pubmed publisher
	Cabello N, GandÃa J, Bertarelli D, Watanabe M, LluÃs C, Franco R, et al. Metabotropic glutamate type 5, dopamine D2 and adenosine A2a receptors form higher-order oligomers in living cells. J Neurochem. 2009;109:1497-507 pubmed publisher
	Gimenez Llort L, Schiffmann S, Shmidt T, Canela L, Camon L, Wassholm M, et al. Working memory deficits in transgenic rats overexpressing human adenosine A2A receptors in the brain. Neurobiol Learn Mem. 2007;87:42-56 pubmed

product information

Catalog Number :

05-717

Subcategory :

Neuroscience

Product Name :

Anti-Adenosine Receptor A2a Antibody, clone 7F6-G5-A2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P29274

Host Name :

Mouse

Antigen :

Adenosine Receptor A2a

Clone :

7F6-G5-A2

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-Adenosine Receptor A2a Antibody, clone 7F6-G5-A2

Cross Reactivity :

Human;Rat;Mouse;Rabbit;Canine

Background :

Adenosine receptors, a class of purinergic G-protein coupled receptors with adenosine as endogenous ligands mediate several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The activity of A2A adenosine receptor is mediated by G proteins which activate adenylyl cyclase. It is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine. Adenosine receptor A2A-R contributes to motoneuron survival by transactivating the tyrosine kinase receptor TrkB and may suggest a new strategy for using this mechanism in neurodegenerative diseases.

ALT Names :

adenosine A2 receptor;adenosine A2a receptor;adenosine receptor subtype A2a

Immunogen :

6His-tagged full-length human recombinant A2A receptor. Epitope mapped to SQPLPGER in the third intracellular loop. Clone 7F6-G5-A2.

Specificity :

This antibody recognizes adenosine receptor A2A, Mr 43 kDa.

Package Size :

200 µg

Uses :

Flow Cytometry;Immunohistochemistry;Western Blotting

Storage :

Stable for 1 year at 2-8°C from date of receipt