Myosin Heavy Chain Antibody, clone A4.1025 | EMD Millipore 05-716 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Myosin Heavy Chain Antibody, clone A4.1025

catalog :

05-716

quantity :

200 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A4.1025

The same clone is also sold as:

Developmental Studies Hybridoma Bank: A4.1025
Santa Cruz Biotechnology: sc-53088

reactivity :

human, mouse, rat, zebrafish

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, blocking or activating experiments

citations: 41

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:200; loading ...; fig s1a western blot; mouse; 1:1000; loading ...; fig 1	Mandai S, Furukawa S, Kodaka M, Hata Y, Mori T, Nomura N, et al. Loop diuretics affect skeletal myoblast differentiation and exercise-induced muscle hypertrophy. Sci Rep. 2017;7:46369 pubmed publisher
western blot; mouse; loading ...; fig 3a	Yang J, Song T, Jo C, Park J, Lee H, Song I, et al. Differential regulation of the histone chaperone HIRA during muscle cell differentiation by a phosphorylation switch. Exp Mol Med. 2016;48:e252 pubmed publisher
western blot; human; fig 1	Sekulic Jablanovic M, Ullrich N, Goldblum D, Palmowski Wolfe A, Zorzato F, Treves S. Functional characterization of orbicularis oculi and extraocular muscles. J Gen Physiol. 2016;147:395-406 pubmed publisher
immunocytochemistry; mouse; fig 6d	Hsu T, Simon L, Neill N, Marcotte R, Sayad A, Bland C, et al. The spliceosome is a therapeutic vulnerability in MYC-driven cancer. Nature. 2015;525:384-8 pubmed publisher
immunohistochemistry; mouse; fig 6d western blot; mouse; fig 6b	Yao X, Tang Z, Fu X, Yin J, Liang Y, Li C, et al. The Mediator subunit MED23 couples H2B mono-ubiquitination to transcriptional control and cell fate determination. EMBO J. 2015;34:2885-902 pubmed publisher
western blot; mouse; fig 4	Rokach O, Sekulic Jablanovic M, Voermans N, Wilmshurst J, Pillay K, Heytens L, et al. Epigenetic changes as a common trigger of muscle weakness in congenital myopathies. Hum Mol Genet. 2015;24:4636-47 pubmed publisher
immunocytochemistry; human; loading ...; fig 2c	Egerman M, Cadena S, Gilbert J, Meyer A, Nelson H, Swalley S, et al. GDF11 Increases with Age and Inhibits Skeletal Muscle Regeneration. Cell Metab. 2015;22:164-74 pubmed publisher
western blot; human	Kraeva N, Heytens L, Jungbluth H, Treves S, Voermans N, Kamsteeg E, et al. Compound RYR1 heterozygosity resulting in a complex phenotype of malignant hyperthermia susceptibility and a core myopathy. Neuromuscul Disord. 2015;25:567-76 pubmed publisher
immunocytochemistry; mouse; fig 4 western blot; mouse; 1:1000; fig 1	Iannotti F, Silvestri C, Mazzarella E, Martella A, Calvigioni D, Piscitelli F, et al. The endocannabinoid 2-AG controls skeletal muscle cell differentiation via CB1 receptor-dependent inhibition of Kv7 channels. Proc Natl Acad Sci U S A. 2014;111:E2472-81 pubmed publisher
western blot; human	Boccafoschi F, Ramella M, Sibillano T, De Caro L, Giannini C, Comparelli R, et al. Human elastin polypeptides improve the biomechanical properties of three-dimensional matrices through the regulation of elastogenesis. J Biomed Mater Res A. 2015;103:1218-30 pubmed publisher
immunocytochemistry; mouse	Lin L, Xu J, Ye Y, Ge J, Zou Y, Liu X. Isosorbide dinitrate inhibits mechanical stress-induced cardiac hypertrophy and autophagy through downregulation of angiotensin II type 1 receptor. J Cardiovasc Pharmacol. 2015;65:1-7 pubmed publisher
immunohistochemistry - paraffin section; mouse; fig 1	Zhou H, Wan B, Grubisic I, Kaplan T, Tjian R. TAF7L modulates brown adipose tissue formation. elife. 2014;3: pubmed publisher
immunocytochemistry; rat	Lin L, Tang C, Xu J, Ye Y, Weng L, Wei W, et al. Mechanical stress triggers cardiomyocyte autophagy through angiotensin II type 1 receptor-mediated p38MAP kinase independently of angiotensin II. PLoS ONE. 2014;9:e89629 pubmed publisher
blocking or activating experiments; mouse	Minetti G, Feige J, Bombard F, Heier A, Morvan F, Nurnberg B, et al. G?i2 signaling is required for skeletal muscle growth, regeneration, and satellite cell proliferation and differentiation. Mol Cell Biol. 2014;34:619-30 pubmed publisher
western blot; human	Yu Y, Qi L, Wu J, Wang Y, Fang W, Zhang H. Kindlin 2 regulates myogenic related factor myogenin via a canonical Wnt signaling in myogenic differentiation. PLoS ONE. 2013;8:e63490 pubmed publisher
	Lin C, Yoshida M, Li L, Ikenaka A, Oshima S, Nakagawa K, et al. iPSC-derived functional human neuromuscular junctions model the pathophysiology of neuromuscular diseases. JCI Insight. 2019;4: pubmed publisher
	Servián Morilla E, Cabrera Serrano M, Rivas Infante E, Carvajal A, Lamont P, Pelayo Negro A, et al. Altered myogenesis and premature senescence underlie human TRIM32-related myopathy. Acta Neuropathol Commun. 2019;7:30 pubmed publisher
	Kolliopoulos C, Raja E, Razmara M, Heldin P, Heldin C, Moustakas A, et al. Transforming growth factor β (TGFβ) induces NUAK kinase expression to fine-tune its signaling output. J Biol Chem. 2019;294:4119-4136 pubmed publisher
	Bai Y, Guo D, Sun X, Tang G, Liao T, Peng Y, et al. Balanced Rac1 activity controls formation and maintenance of neuromuscular acetylcholine receptor clusters. J Cell Sci. 2018;131: pubmed publisher
	Dammone G, Karaz S, Lukjanenko L, Winkler C, Sizzano F, Jacot G, et al. PPARγ Controls Ectopic Adipogenesis and Cross-Talks with Myogenesis During Skeletal Muscle Regeneration. Int J Mol Sci. 2018;19: pubmed publisher
	Mandai S, Mori T, Nomura N, Furusho T, Arai Y, Kikuchi H, et al. WNK1 regulates skeletal muscle cell hypertrophy by modulating the nuclear localization and transcriptional activity of FOXO4. Sci Rep. 2018;8:9101 pubmed publisher
	Cruz J, Hupper N, Wilson L, Concannon J, Wang Y, Oberhauser B, et al. Protein kinase A activation inhibits DUX4 gene expression in myotubes from patients with facioscapulohumeral muscular dystrophy. J Biol Chem. 2018;293:11837-11849 pubmed publisher
	Yang Y, Li H, Cao X, Qiao C, Ya J. Septation of the Intrapericardial Arterial Trunks in the Early Human Embryonic Heart. Chin Med J (Engl). 2018;131:1457-1464 pubmed publisher
	Yoneyama Y, Lanzerstorfer P, Niwa H, Umehara T, Shibano T, Yokoyama S, et al. IRS-1 acts as an endocytic regulator of IGF-I receptor to facilitate sustained IGF signaling. elife. 2018;7: pubmed publisher
	Mueller A, Cichewicz M, Dey B, Layer R, Reon B, Gagan J, et al. MUNC, a long noncoding RNA that facilitates the function of MyoD in skeletal myogenesis. Mol Cell Biol. 2015;35:498-513 pubmed publisher
	Rabiee F, Forouzanfar M, Ghazvini Zadegan F, Tanhaei S, Ghaedi K, Motovali Bashi M, et al. Induced expression of Fndc5 significantly increased cardiomyocyte differentiation rate of mouse embryonic stem cells. Gene. 2014;551:127-37 pubmed publisher
	Wu Y, Long Q, Zheng Z, Xia Q, Wen F, Zhu X, et al. Adipose induces myoblast differentiation and mediates TNF?-regulated myogenesis. Biochim Biophys Acta. 2014;1839:1183-95 pubmed publisher
	Wan X, Liu L, Ding X, Zhou P, Yuan X, Zhou Z, et al. Mll2 controls cardiac lineage differentiation of mouse embryonic stem cells by promoting H3K4me3 deposition at cardiac-specific genes. Stem Cell Rev. 2014;10:643-52 pubmed publisher
	Bareja A, Holt J, Luo G, Chang C, Lin J, Hinken A, et al. Human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis. PLoS ONE. 2014;9:e90398 pubmed publisher
	de Carlos F, Cobo J, Macias E, Feito J, Cobo T, Calavia M, et al. The sensory innervation of the human pharynx: searching for mechanoreceptors. Anat Rec (Hoboken). 2013;296:1735-46 pubmed publisher
	Chen H, Chen C, Lin Y, Chen Y, Chen W, Chen C. Proteomic analysis related to stress urinary incontinence following vaginal trauma in female mice. Eur J Obstet Gynecol Reprod Biol. 2013;171:171-9 pubmed publisher
	Rokach O, Ullrich N, Rausch M, Mouly V, Zhou H, Muntoni F, et al. Establishment of a human skeletal muscle-derived cell line: biochemical, cellular and electrophysiological characterization. Biochem J. 2013;455:169-77 pubmed publisher
	Liu J, Xu H, Zhou H, Weir M, Chen Q, TROTMAN C. Human umbilical cord stem cell encapsulation in novel macroporous and injectable fibrin for muscle tissue engineering. Acta Biomater. 2013;9:4688-97 pubmed publisher
	Hoogaars W, Mouisel E, Pasternack A, Hulmi J, Relizani K, Schuelke M, et al. Combined effect of AAV-U7-induced dystrophin exon skipping and soluble activin Type IIB receptor in mdx mice. Hum Gene Ther. 2012;23:1269-79 pubmed publisher
	Dimitrakis P, Romay Ogando M, Timolati F, Suter T, Zuppinger C. Effects of doxorubicin cancer therapy on autophagy and the ubiquitin-proteasome system in long-term cultured adult rat cardiomyocytes. Cell Tissue Res. 2012;350:361-72 pubmed publisher
	Wang L, Chen X, Zheng Y, Li F, Lu Z, Chen C, et al. MiR-23a inhibits myogenic differentiation through down regulation of fast myosin heavy chain isoforms. Exp Cell Res. 2012;318:2324-34 pubmed publisher
	Liu J, Zhou H, Weir M, Xu H, Chen Q, TROTMAN C. Fast-degradable microbeads encapsulating human umbilical cord stem cells in alginate for muscle tissue engineering. Tissue Eng Part A. 2012;18:2303-14 pubmed publisher
	Wang J, Li X, Yang X, Sun Q, Huang R, Xing J, et al. Maternal dietary protein induces opposite myofiber type transition in Meishan pigs at weaning and finishing stages. Meat Sci. 2011;89:221-7 pubmed publisher
	Lin L, Gong H, Ge J, Jiang G, Zhou N, Li L, et al. High density lipoprotein downregulates angiotensin II type 1 receptor and inhibits angiotensin II-induced cardiac hypertrophy. Biochem Biophys Res Commun. 2011;404:28-33 pubmed publisher
	Munro S, Khaire N, Inche A, Carr S, La Thangue N. Lysine methylation regulates the pRb tumour suppressor protein. Oncogene. 2010;29:2357-67 pubmed publisher
	Shu L, Houghton P. The mTORC2 complex regulates terminal differentiation of C2C12 myoblasts. Mol Cell Biol. 2009;29:4691-700 pubmed publisher

product information

Catalog Number :

05-716

Subcategory :

Metabolism

Product Name :

Anti-Myosin Heavy Chain Antibody, clone A4.1025

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P12882

Host Name :

Mouse

Antigen :

Myosin Heavy Chain

Clone :

A4.1025

Conjugate :

Ascites

Isotype :

IgG

Product Description :

Anti-Myosin Heavy Chain Antibody, clone A4.1025

Cross Reactivity :

Human;Rat;Mouse;Zebrafish;Drosophila;Rabbit

Background :

Myosin is one of the two major components of muscle tissue responsible for contraction. In muscle cells myosin is arranged as thick filamentous structures that are arranged in parallel to thin filamentous structures composed of actin. Muscle contraction is actuated by the coupling and uncoupling of thin and thick filaments through a chemical linkage mediated by ATP. The coupling and uncoupling is arranged such that the filaments slide in one direction when coupled and in reverse direction when uncoupled or released. Myosin and actin also serve to facilitate motility in non-muscle cells such as in slime molds where shorter yet similar filamentous structures work together. An increasing number of different myosins are being described. Myosin I is a low molecular weight myosin found in protozoa and does not self-assemble. Myosin II is the classic form found in muscle cells that self assemble into thick bundles. Myosin II is a multimeric protein that is composed of two heavy chains and two light chains.

ALT Names :

Myosin heavy chain 1;Myosin heavy chain 2x;Myosin heavy chain IIx/d;Myosin heavy chain, skeletal muscle, adult 1;myosin, heavy chain 1, skeletal muscle, adult;myosin, heavy polypeptide 1, skeletal muscle, adult

Immunogen :

Partially purified protein from adult human skeletal muscle myosin. Clone A4.1025.

Specificity :

This antibody recognizes myosin heavy chain, Mr ~220 kDa. Immunoblotting with muscle cell lysates will often show several close bands from different myosin heavy chain genes, as well as degradation products.

Package Size :

200 µL

Uses :

Immunohistochemistry;Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.