HP1γ Antibody, clone 42s2 | EMD Millipore 05-690 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

HP1γ Antibody, clone 42s2

catalog :

05-690

quantity :

200 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

42s2

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, chromatin immunoprecipitation, RNA immunoprecipitation

citations: 19

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:5000; fig 4a	Klein M, Dickson M, Antonescu C, Qin L, Dooley S, Barlas A, et al. PDLIM7 and CDH18 regulate the turnover of MDM2 during CDK4/6 inhibitor therapy-induced senescence. Oncogene. 2018;37:5066-5078 pubmed publisher
western blot; human; 1:1000; loading ...; fig 5g	Bot C, Pfeiffer A, Giordano F, Manjeera D, Dantuma N, Ström L. Independent mechanisms recruit the cohesin loader protein NIPBL to sites of DNA damage. J Cell Sci. 2017;130:1134-1146 pubmed publisher
chromatin immunoprecipitation; human; loading ...; fig 1a western blot; human; loading ...; fig 1c	Wang Y, Wang Y, Ma L, Nie M, Ju J, Liu M, et al. Heterochromatin Protein 1Î³ Is a Novel Epigenetic Repressor of Human Embryonic Ïµ-Globin Gene Expression. J Biol Chem. 2017;292:4811-4817 pubmed publisher
RNA immunoprecipitation; human; fig 3	G Hendrickson D, Kelley D, Tenen D, BERNSTEIN B, Rinn J. Widespread RNA binding by chromatin-associated proteins. Genome Biol. 2016;17:28 pubmed publisher
chromatin immunoprecipitation; human	Huichalaf C, Micheloni S, Ferri G, Caccia R, Gabellini D. DNA methylation analysis of the macrosatellite repeat associated with FSHD muscular dystrophy at single nucleotide level. PLoS ONE. 2014;9:e115278 pubmed publisher
chromatin immunoprecipitation; human; fig 6	Harouz H, Rachez C, Meijer B, Marteyn B, Donnadieu F, Cammas F, et al. Shigella flexneri targets the HP1Î³ subcode through the phosphothreonine lyase OspF. EMBO J. 2014;33:2606-22 pubmed publisher
western blot; human; 1:500	Cao Q, Wang X, Zhao M, Yang R, Malik R, Qiao Y, et al. The central role of EED in the orchestration of polycomb group complexes. Nat Commun. 2014;5:3127 pubmed publisher
chromatin immunoprecipitation; mouse	Liu B, Tahk S, Yee K, Fan G, Shuai K. The ligase PIAS1 restricts natural regulatory T cell differentiation by epigenetic repression. Science. 2010;330:521-5 pubmed publisher
	Sridharan R, Gonzales Cope M, Chronis C, Bonora G, McKee R, Huang C, et al. Proteomic and genomic approaches reveal critical functions of H3K9 methylation and heterochromatin protein-1? in reprogramming to pluripotency. Nat Cell Biol. 2013;15:872-82 pubmed publisher
	Witham J, Ouboussad L, Lefevre P. A NF-?B-dependent dual promoter-enhancer initiates the lipopolysaccharide-mediated transcriptional activation of the chicken lysozyme in macrophages. PLoS ONE. 2013;8:e59389 pubmed publisher
	He S, Khan D, Winter S, Seiser C, Davie J. Dynamic distribution of HDAC1 and HDAC2 during mitosis: association with F-actin. J Cell Physiol. 2013;228:1525-35 pubmed publisher
	Choi J, Park M, Lee J. Suppression and recovery of BRCA1-mediated transcription by HP1? via modulation of promoter occupancy. Nucleic Acids Res. 2012;40:11321-38 pubmed publisher
	Thorne J, Ouboussad L, Lefevre P. Heterochromatin protein 1 gamma and I?B kinase alpha interdependence during tumour necrosis factor gene transcription elongation in activated macrophages. Nucleic Acids Res. 2012;40:7676-89 pubmed publisher
	Sustáčková G, Kozubek S, Stixova L, Legartova S, Matula P, Orlova D, et al. Acetylation-dependent nuclear arrangement and recruitment of BMI1 protein to UV-damaged chromatin. J Cell Physiol. 2012;227:1838-50 pubmed publisher
	Ferenczy M, Ranayhossaini D, DeLuca N. Activities of ICP0 involved in the reversal of silencing of quiescent herpes simplex virus 1. J Virol. 2011;85:4993-5002 pubmed publisher
	Ferenczy M, DeLuca N. Reversal of heterochromatic silencing of quiescent herpes simplex virus type 1 by ICP0. J Virol. 2011;85:3424-35 pubmed publisher
	Higo S, Asano Y, Kato H, Yamazaki S, Nakano A, Tsukamoto O, et al. Isoform-specific intermolecular disulfide bond formation of heterochromatin protein 1 (HP1). J Biol Chem. 2010;285:31337-47 pubmed publisher
	Anelli V, Santoriello C, Distel M, Köster R, Ciccarelli F, Mione M. Global repression of cancer gene expression in a zebrafish model of melanoma is linked to epigenetic regulation. Zebrafish. 2009;6:417-24 pubmed publisher
	Ferenczy M, DeLuca N. Epigenetic modulation of gene expression from quiescent herpes simplex virus genomes. J Virol. 2009;83:8514-24 pubmed publisher

product information

Catalog Number :

05-690

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-HP1γ Antibody, clone 42s2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q13185

Host Name :

Mouse

Antigen :

HP1γ

Clone :

42s2

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-HP1γ Antibody, clone 42s2

Cross Reactivity :

Human;Mouse

Background :

Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated

ALT Names :

HP1 gamma;HP1 gamma homolog;Heterochromatin protein 1 homolog gamma;Modifier 2 protein;chromobox homolog 3;chromobox homolog 3 (Drosophila HP1 gamma);chromobox homolog 3 (HP1 gamma homolog, Drosophila);heterochromatin protein HP1 gamma;heterochromatin-like protein 1

Immunogen :

GST fusion protein corresponding to residues 17-173 of human HP1γ protein. Clone 42s2.

Specificity :

This antibody recognizes HP1γ protein, Mr ~25 kDa.

Package Size :

200 µg

Uses :

Immunohistochemistry;Western Blotting;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.