phospho-Src (Tyr416) Antibody, clone 9A6 | EMD Millipore 05-677 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-Src (Tyr416) Antibody, clone 9A6

catalog :

05-677

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

9A6

The same clone is also sold as:

OriGene: AM00145BT-N, AM00145PU-N
Santa Cruz Biotechnology: sc-81521

reactivity :

human

application :

western blot, immunoprecipitation

citations: 15

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...	Waters A, Stewart J, Atigadda V, Mroczek Musulman E, Muccio D, Grubbs C, et al. Preclinical Evaluation of UAB30 in Pediatric Renal and Hepatic Malignancies. Mol Cancer Ther. 2016;15:911-21 pubmed publisher
western blot; human; fig 7	Yen Y, Hsiao J, Jiang S, Chang J, Wang S, Shen Y, et al. Insulin-like growth factor-independent insulin-like growth factor binding protein 3 promotes cell migration and lymph node metastasis of oral squamous cell carcinoma cells by requirement of integrin β1. Oncotarget. 2015;6:41837-55 pubmed publisher
western blot; human; tbl s3	Cheng H, Liang Y, Kuo Y, Chuu C, Lin C, Lee M, et al. Identification of thioridazine, an antipsychotic drug, as an antiglioblastoma and anticancer stem cell agent using public gene expression data. Cell Death Dis. 2015;6:e1753 pubmed publisher
western blot; human; loading ...; fig s1	Megison M, Gillory L, Stewart J, Nabers H, Mrozcek Musulman E, Beierle E. FAK inhibition abrogates the malignant phenotype in aggressive pediatric renal tumors. Mol Cancer Res. 2014;12:514-26 pubmed publisher
	Kamekura R, Kolegraff K, Nava P, Hilgarth R, Feng M, Parkos C, et al. Loss of the desmosomal cadherin desmoglein-2 suppresses colon cancer cell proliferation through EGFR signaling. Oncogene. 2014;33:4531-6 pubmed publisher
	Miekus K, Lukasiewicz E, Jarocha D, Sekula M, Drabik G, Majka M. The decreased metastatic potential of rhabdomyosarcoma cells obtained through MET receptor downregulation and the induction of differentiation. Cell Death Dis. 2013;4:e459 pubmed publisher
	Duong V, Jaglal M, Zhang L, Kale V, Lancet J, Komrokji R, et al. Phase II pilot study of oral dasatinib in patients with higher-risk myelodysplastic syndrome (MDS) who failed conventional therapy. Leuk Res. 2013;37:300-4 pubmed publisher
	O Reilly L, Zhang X, Smithgall T. Individual Src-family tyrosine kinases direct the degradation or protection of the clock protein Timeless via differential ubiquitylation. Cell Signal. 2013;25:860-6 pubmed publisher
	Cipolla L, Consonni A, Guidetti G, Canobbio I, Okigaki M, Falasca M, et al. The proline-rich tyrosine kinase Pyk2 regulates platelet integrin ?IIb?3 outside-in signaling. J Thromb Haemost. 2013;11:345-56 pubmed publisher
	Wheeler S, Morariu E, Bednash J, Otte C, Seethala R, Chiosea S, et al. Lyn kinase mediates cell motility and tumor growth in EGFRvIII-expressing head and neck cancer. Clin Cancer Res. 2012;18:2850-60 pubmed publisher
	Alvarez D, Agaisse H. Casein kinase 2 regulates vaccinia virus actin tail formation. Virology. 2012;423:143-51 pubmed publisher
	Mital J, Miller N, Fischer E, Hackstadt T. Specific chlamydial inclusion membrane proteins associate with active Src family kinases in microdomains that interact with the host microtubule network. Cell Microbiol. 2010;12:1235-49 pubmed publisher
	Sirvent A, Benistant C, Pannequin J, Veracini L, Simon V, Bourgaux J, et al. Src family tyrosine kinases-driven colon cancer cell invasion is induced by Csk membrane delocalization. Oncogene. 2010;29:1303-15 pubmed publisher
	Koreckij T, Nguyen H, Brown L, Yu E, Vessella R, Corey E. Dasatinib inhibits the growth of prostate cancer in bone and provides additional protection from osteolysis. Br J Cancer. 2009;101:263-8 pubmed publisher
	Toi S, Shibata N, Sawada T, Kobayashi M, Uchiyama S. Activation of the non-receptor tyrosine kinase cSrc in macrophage-rich atherosclerotic plaques of human carotid arteries. Acta Histochem Cytochem. 2007;40:153-61 pubmed publisher

product information

Catalog Number :

05-677

Subcategory :

Cell Signaling

Product Name :

Anti-phospho-Src (Tyr416) Antibody, clone 9A6

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P12931

Host Name :

Mouse

Antigen :

phospho-Src (Tyr416)

Clone :

clone 9A6

Conjugate :

Purified

Isotype :

IgG3

Product Description :

Anti-phospho-Src (Tyr416) Antibody, clone 9A6

Cross Reactivity :

Human;Mouse

Background :

Src (also known as pp60src) is a non receptor Tyrosine Kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. There are two critical phosphorylation sites of tyrosine on Src, tyrosine 418 and tyrosine 529 (referring to human Src sequence). The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated.

ALT Names :

proto-oncogene tyrosine-protein kinase SRC;protooncogene SRC, Rous sarcoma;tyrosine kinase pp60c-src;tyrosine-protein kinase SRC-1;v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog;v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)

Immunogen :

KLH-conjugated, synthetic peptide containing phosphotyrosine in the sequence context corresponding to tyrosine 416 of avian Src. Clone 9A6.

Specificity :

Recognizes phospho-Src, Mr 60kDa. The antibody does not crossreact with the nonphosphorylated form of Src or with unrelated phosphorylation sites. Predicted to recognize all Src family members phosphorylated at the tyrosine corresponding to Tyr416 of avian Src.

Package Size :

100 μg

Uses :

Western Blotting;Immunoprecipitation

Storage :

Stable for 1 year at from date of receipt.