phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6 | EMD Millipore 05-669 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6

catalog :

05-669

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

11E6

The same clone is also sold as:

Santa Cruz Biotechnology: sc-81433

reactivity :

human, mouse, rat, dogs

application :

western blot, ELISA, immunocytochemistry

citations: 16

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:1000; fig 2e	Chen K, Tsai M, Wu C, Jou M, Wei I, Huang C. AMPA Receptor-mTOR Activation is Required for the Antidepressant-Like Effects of Sarcosine during the Forced Swim Test in Rats: Insertion of AMPA Receptor may Play a Role. Front Behav Neurosci. 2015;9:162 pubmed publisher
western blot; human; tbl s3	Cheng H, Liang Y, Kuo Y, Chuu C, Lin C, Lee M, et al. Identification of thioridazine, an antipsychotic drug, as an antiglioblastoma and anticancer stem cell agent using public gene expression data. Cell Death Dis. 2015;6:e1753 pubmed publisher
immunocytochemistry; human western blot; human	Stergiou L, Bauer M, Mair W, Bausch Fluck D, Drayman N, Wollscheid B, et al. Integrin-mediated signaling induced by simian virus 40 leads to transient uncoupling of cortical actin and the plasma membrane. PLoS ONE. 2013;8:e55799 pubmed publisher
	Zhuang Y, Chan D, Haugrud A, Miskimins W. Mechanisms by which low glucose enhances the cytotoxicity of metformin to cancer cells both in vitro and in vivo. PLoS ONE. 2014;9:e108444 pubmed publisher
	Dong C, Rovnaghi C, Anand K. Ketamine affects the neurogenesis of rat fetal neural stem progenitor cells via the PI3K/Akt-p27 signaling pathway. Birth Defects Res B Dev Reprod Toxicol. 2014;101:355-63 pubmed publisher
	Chen Y, Lu H, Hsu S, Kuo C, Chang S, Lin J, et al. Bufalin inhibits migration and invasion in human hepatocellular carcinoma SK-Hep1 cells through the inhibitions of NF-kB and matrix metalloproteinase-2/-9-signaling pathways. Environ Toxicol. 2015;30:74-82 pubmed publisher
	Lu C, Yang J, Chiang J, Hour M, Amagaya S, Lu K, et al. Inhibition of invasion and migration by newly synthesized quinazolinone MJ-29 in human oral cancer CAL 27 cells through suppression of MMP-2/9 expression and combined down-regulation of MAPK and AKT signaling. Anticancer Res. 2012;32:2895-903 pubmed
	Jones L, Antonelli J, Masko E, Broadwater G, Lascola C, Fels D, et al. Exercise modulation of the host-tumor interaction in an orthotopic model of murine prostate cancer. J Appl Physiol (1985). 2012;113:263-72 pubmed publisher
	Biensø R, Ringholm S, Kiilerich K, Aachmann Andersen N, Krogh Madsen R, Guerra B, et al. GLUT4 and glycogen synthase are key players in bed rest-induced insulin resistance. Diabetes. 2012;61:1090-9 pubmed publisher
	Chen Y, Chen Y, Hsu Y, Chang W, Hsiao C, Min M, et al. Akt1 deficiency modulates reward learning and reward prediction error in mice. Genes Brain Behav. 2012;11:157-69 pubmed publisher
	Matsuoka Y, Sasaki Y, Nakatsuka R, Takahashi M, Iwaki R, Uemura Y, et al. Low level of c-kit expression marks deeply quiescent murine hematopoietic stem cells. Stem Cells. 2011;29:1783-91 pubmed publisher
	Pulinilkunnil T, He H, Kong D, Asakura K, Peroni O, Lee A, et al. Adrenergic regulation of AMP-activated protein kinase in brown adipose tissue in vivo. J Biol Chem. 2011;286:8798-809 pubmed publisher
	Sei Y, Li Z, Song J, Ren Patterson R, Tunbridge E, Iizuka Y, et al. Epistatic and functional interactions of catechol-o-methyltransferase (COMT) and AKT1 on neuregulin1-ErbB signaling in cell models. PLoS ONE. 2010;5:e10789 pubmed publisher
	Deng K, Gao Y, Cao Z, Graziani E, Wood A, Doherty P, et al. Overcoming amino-Nogo-induced inhibition of cell spreading and neurite outgrowth by 12-O-tetradecanoylphorbol-13-acetate-type tumor promoters. J Biol Chem. 2010;285:6425-33 pubmed publisher
	Chang M, Chen C, Hsieh C, Lee C, Su Y, Hu Y, et al. Mesothelin inhibits paclitaxel-induced apoptosis through the PI3K pathway. Biochem J. 2009;424:449-58 pubmed publisher
	Sei Y, Ren Patterson R, Li Z, Tunbridge E, Egan M, Kolachana B, et al. Neuregulin1-induced cell migration is impaired in schizophrenia: association with neuregulin1 and catechol-o-methyltransferase gene polymorphisms. Mol Psychiatry. 2007;12:946-57 pubmed

product information

Catalog Number :

05-669

Subcategory :

Cell Signaling

Product Name :

Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P31749

Host Name :

Mouse

Antigen :

phospho-Akt1/PKBα (Ser473)

Clone :

11E6

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6

Cross Reactivity :

Mouse;Rat;Human;Canine

Background :

The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB-β or RacPK-β) and Akt3 (also designated PKB-γ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the 85 kDa subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1- stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin. Taken together, this data strongly suggests that the protein signals downstream of the PI kinases.

ALT Names :

Protein kinase B;RAC-alpha serine/threonine-protein kinase;murine thymoma viral (v-akt) oncogene homolog-1;rac protein kinase alpha;v-akt murine thymoma viral oncogene homolog 1

Immunogen :

KLH conjugated synthetic peptide containing a pSer that corresponds to amino acid residues around phosphoserine 473.

Specificity :

Recognizes phosphorylated Akt1/PKBα at Ser473, Mr 60 kDa. This lot of antibody does not cross-react with Akt1/PKBα dephosphorylated at Ser473.

Package Size :

100 µg

Uses :

ELISA;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Reconstitute prior to use. Reconstitute with 1 mL H 2 O for 15 min at room temperature. Reconstituted aliquots should be stored frozen at -80°C. Thawed aliquots may be stored at 2-8°C for up to 3 months. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.