phospho-CREB (Ser133) Antibody, clone 10E9 | EMD Millipore 05-667 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-CREB (Ser133) Antibody, clone 10E9

catalog :

05-667

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

10E9

The same clone is also sold as:

Santa Cruz Biotechnology: sc-81486
Invitrogen: MA1-114

reactivity :

human, mouse, rat, dogs

application :

western blot, ELISA, immunocytochemistry

citations: 12

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; 1:500; fig 6	Buren C, Tu G, Parsons M, Sepers M, Raymond L. Influence of cortical synaptic input on striatal neuronal dendritic arborization and sensitivity to excitotoxicity in corticostriatal coculture. J Neurophysiol. 2016;116:380-90 pubmed publisher
immunocytochemistry; rat; 1:1000	Dorris D, Hauser C, Minnehan C, Meitzen J. An aerator for brain slice experiments in individual cell culture plate wells. J Neurosci Methods. 2014;238:1-10 pubmed publisher
	Buren C, Wang L, Smith Dijak A, Raymond L. Region-specific pro-survival signaling and global neuronal protection by wild-type Huntingtin. J Huntingtons Dis. 2014;3:365-76 pubmed publisher
	Meitzen J, Luoma J, Boulware M, Hedges V, Peterson B, Tuomela K, et al. Palmitoylation of estrogen receptors is essential for neuronal membrane signaling. Endocrinology. 2013;154:4293-304 pubmed publisher
	Chang Y, Yu Y, Shew J, Lee W, Hwang J, Chen Y, et al. Deficiency of NPGPx, an oxidative stress sensor, leads to obesity in mice and human. EMBO Mol Med. 2013;5:1165-79 pubmed publisher
	Law N, Weck J, Kyriss B, Nilson J, Hunzicker Dunn M. Lhcgr expression in granulosa cells: roles for PKA-phosphorylated ?-catenin, TCF3, and FOXO1. Mol Endocrinol. 2013;27:1295-310 pubmed publisher
	Milnerwood A, Kaufman A, Sepers M, Gladding C, Zhang L, Wang L, et al. Mitigation of augmented extrasynaptic NMDAR signaling and apoptosis in cortico-striatal co-cultures from Huntington's disease mice. Neurobiol Dis. 2012;48:40-51 pubmed publisher
	Kaufman A, Milnerwood A, Sepers M, Coquinco A, She K, Wang L, et al. Opposing roles of synaptic and extrasynaptic NMDA receptor signaling in cocultured striatal and cortical neurons. J Neurosci. 2012;32:3992-4003 pubmed publisher
	Hutchinson A, Deng J, Aryal D, Wetsel W, West A. Differential regulation of MeCP2 phosphorylation in the CNS by dopamine and serotonin. Neuropsychopharmacology. 2012;37:321-37 pubmed publisher
	Spiga F, Liu Y, Aguilera G, Lightman S. Temporal effect of adrenocorticotrophic hormone on adrenal glucocorticoid steroidogenesis: involvement of the transducer of regulated cyclic AMP-response element-binding protein activity. J Neuroendocrinol. 2011;23:136-42 pubmed publisher
	Pearce L, Alton G, Richter D, Kath J, Lingardo L, Chapman J, et al. Characterization of PF-4708671, a novel and highly specific inhibitor of p70 ribosomal S6 kinase (S6K1). Biochem J. 2010;431:245-55 pubmed publisher
	Fuxe J, Lashnits E, O Brien S, Baluk P, Tabruyn S, Kuhnert F, et al. Angiopoietin/Tie2 signaling transforms capillaries into venules primed for leukocyte trafficking in airway inflammation. Am J Pathol. 2010;176:2009-18 pubmed publisher

product information

Catalog Number :

05-667

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-phospho-CREB (Ser133) Antibody, clone 10E9

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P16220

Host Name :

Mouse

Antigen :

phospho-CREB (Ser133)

Clone :

clone 10E9

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-phospho-CREB (Ser133) Antibody, clone 10E9

Cross Reactivity :

Human;Mouse;Rat;Canine

Background :

CREB is a beta ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2.

ALT Names :

active transcription factor CREB;cAMP responsive element binding protein 1;cAMP-response element-binding protein-1;transactivator protein

Immunogen :

KLH-conjugated, synthetic peptide (REILSRRP(pS)YR) corresponding to amino acids 125-135 of human CREB. The immunizing sequence is identical in human and mouse. Clone 10E9.

Specificity :

Recognizes phosphorylated CREB at Mr 46 kDa. May also recognize p30 and p38 proteins, which likely correspond to phosphorylated CREM and ATF-1 respectively, since the two proteins share significant homology with the phosphorylated CREB immunizing peptide

Package Size :

100 μg

Uses :

Western Blotting;ELISA

Storage :

Lyophilized: Stable for 1 year at -20ºC from date of receipt. Rehydrated: Stable for 3 months at 2-8ºC. Aliquot solution to store frozen and avoid repeated freeze thaw cycles.