Active-β-Catenin (ABC) Antibody, clone 8E7 | EMD Millipore 05-665 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Active-β-Catenin (ABC) Antibody, clone 8E7

catalog :

05-665

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8E7

reactivity :

African green monkey, brownbanded bambooshark, human, mouse, rat, zebrafish , Xenopus laevis, rhesus macaque

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, other

citations: 144

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; loading ...; fig 3k western blot; mouse; 1:1000; loading ...; fig 3f western blot; human; 1:1000; loading ...; fig 3i	Zhu K, Lai Y, Cao H, Bai X, Liu C, Yan Q, et al. Kindlin-2 modulates MafA and β-catenin expression to regulate β-cell function and mass in mice. Nat Commun. 2020;11:484 pubmed publisher
western blot; human; loading ...; fig 6b	Nakanishi M, Mitchell R, Benoit Y, Orlando L, Reid J, Shimada K, et al. Human Pluripotency Is Initiated and Preserved by a Unique Subset of Founder Cells. Cell. 2019;177:910-924.e22 pubmed publisher
western blot; human; loading ...; fig 1d	Zhou L, Chen X, Lu M, Wu Q, Yuan Q, Hu C, et al. Wnt/β-catenin links oxidative stress to podocyte injury and proteinuria. Kidney Int. 2019;95:830-845 pubmed publisher
western blot; rat; loading ...; fig 4a	Chen Z, Li Y, Jiang G, Yang C, Wang Y, Wang X, et al. Knockdown of LRP6 activates Drp1 to inhibit survival of cardiomyocytes during glucose deprivation. Biomed Pharmacother. 2018;103:1408-1414 pubmed publisher
western blot; human; 1:1000; fig 2b	Monteagudo S, Cornelis F, Aznar López C, Yibmantasiri P, Guns L, Carmeliet P, et al. DOT1L safeguards cartilage homeostasis and protects against osteoarthritis. Nat Commun. 2017;8:15889 pubmed publisher
western blot; Xenopus laevis; 1:500; loading ...; fig 4b	Rauschenberger V, Bernkopf D, Krenn S, Jalal K, Heller J, Behrens J, et al. The phosphatase Pgam5 antagonizes Wnt/β-Catenin signaling in embryonic anterior-posterior axis patterning. Development. 2017;144:2234-2247 pubmed publisher
western blot; human; 1:1000; loading ...; fig 5d	Cao P, Aoki Y, Badri L, Walker N, Manning C, Lagstein A, et al. Autocrine lysophosphatidic acid signaling activates ?-catenin and promotes lung allograft fibrosis. J Clin Invest. 2017;127:1517-1530 pubmed publisher
western blot; mouse; 1:1000; fig 4e	Subramaniam M, Cicek M, Pitel K, Bruinsma E, Nelson Holte M, Withers S, et al. TIEG1 modulates ?-catenin sub-cellular localization and enhances Wnt signaling in bone. Nucleic Acids Res. 2017;45:5170-5182 pubmed publisher
immunohistochemistry - paraffin section; rat; 1:50 immunohistochemistry; rat; loading ...; fig 8	Furukawa S, Nagaike M, Ozaki K. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol. 2017;30:79-107 pubmed publisher
western blot; mouse; 1:1000; fig 6d	Mindos T, Dun X, North K, Doddrell R, Schulz A, Edwards P, et al. Merlin controls the repair capacity of Schwann cells after injury by regulating Hippo/YAP activity. J Cell Biol. 2017;216:495-510 pubmed publisher
immunohistochemistry; mouse; 1:500; loading ...; fig 4d	Foerster P, Daclin M, Asm S, Faucourt M, Boletta A, Genovesio A, et al. mTORC1 signaling and primary cilia are required for brain ventricle morphogenesis. Development. 2017;144:201-210 pubmed publisher
immunocytochemistry; mouse; loading ...; fig 4c western blot; mouse; loading ...; fig 4b western blot; human; loading ...; fig 4a	Baarsma H, Skronska Wasek W, Mutze K, Ciolek F, Wagner D, John Schuster G, et al. Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD. J Exp Med. 2017;214:143-163 pubmed publisher
immunocytochemistry; human; loading ...; fig e5e	Harper K, Sosa M, Entenberg D, Hosseini H, Cheung J, Nobre R, et al. Mechanism of early dissemination and metastasis in Her2+ mammary cancer. Nature. 2016;540:588-592 pubmed publisher
immunohistochemistry - paraffin section; brownbanded bambooshark; 1:250; loading ...; fig 6c	Atkinson C, Martin K, Fraser G, Collin S. Morphology and distribution of taste papillae and oral denticles in the developing oropharyngeal cavity of the bamboo shark, Chiloscyllium punctatum. Biol Open. 2016;5:1759-1769 pubmed publisher
western blot; mouse; 1:1000; fig s5c	Beyer S, Pontis J, Schirwis E, Battisti V, Rudolf A, Le Grand F, et al. Canonical Wnt signalling regulates nuclear export of Setdb1 during skeletal muscle terminal differentiation. Cell Discov. 2016;2:16037 pubmed
western blot; human; 1:1000; fig 4c	Huang T, Alvarez A, Pangeni R, Horbinski C, Lu S, Kim S, et al. A regulatory circuit of miR-125b/miR-20b and Wnt signalling controls glioblastoma phenotypes through FZD6-modulated pathways. Nat Commun. 2016;7:12885 pubmed publisher
western blot; human; 1:1000; fig 2c western blot; African green monkey; 1:1000; loading ...; fig 2c western blot; mouse; 1:1000; loading ...; fig 2d	Drelon C, Berthon A, Sahut Barnola I, Mathieu M, Dumontet T, Rodriguez S, et al. PKA inhibits WNT signalling in adrenal cortex zonation and prevents malignant tumour development. Nat Commun. 2016;7:12751 pubmed publisher
immunohistochemistry; human; 1:100; loading ...; fig 4e	Hahn J, McFarland K, Combs K, Supp D. Partial epithelial-mesenchymal transition in keloid scars: regulation of keloid keratinocyte gene expression by transforming growth factor-?1. Burns Trauma. 2016;4:30 pubmed publisher
immunocytochemistry; human; 1:100; fig 3 western blot; human; 1:1000; fig 3	Talar B, Gajos Michniewicz A, Talar M, Chouaib S, Czyz M. Pentoxifylline Inhibits WNT Signalling in ?-Cateninhigh Patient-Derived Melanoma Cell Populations. PLoS ONE. 2016;11:e0158275 pubmed publisher
western blot; human; fig 4	Li B, Sun J, Dong Z, Xue P, He X, Liao L, et al. GCN5 modulates osteogenic differentiation of periodontal ligament stem cells through DKK1 acetylation in inflammatory microenvironment. Sci Rep. 2016;6:26542 pubmed publisher
western blot; mouse; fig 6	Zeng L, Cai C, Li S, Wang W, Li Y, Chen J, et al. Essential Roles of Cyclin Y-Like 1 and Cyclin Y in Dividing Wnt-Responsive Mammary Stem/Progenitor Cells. PLoS Genet. 2016;12:e1006055 pubmed publisher
western blot; mouse; 1:500; fig 3c	Li N, Liu S, Zhang H, Deng Z, Zhao H, Zhao Q, et al. Exogenous R-Spondin1 Induces Precocious Telogen-to-Anagen Transition in Mouse Hair Follicles. Int J Mol Sci. 2016;17: pubmed publisher
immunocytochemistry; human; 1:1000; fig 5 western blot; human; 1:1000; fig 5	Francis K, Ton A, Xin Y, O Halloran P, Wassif C, Malik N, et al. Modeling Smith-Lemli-Opitz syndrome with induced pluripotent stem cells reveals a causal role for Wnt/Î²-catenin defects in neuronal cholesterol synthesis phenotypes. Nat Med. 2016;22:388-96 pubmed publisher
western blot; human; fig 5	Zhang M, Linghu E, Zhan Q, He T, Cao B, Brock M, et al. Methylation of DACT2 accelerates esophageal cancer development by activating Wnt signaling. Oncotarget. 2016;7:17957-69 pubmed publisher
western blot; mouse; fig 4a	Lee S, Shatadal S, Griep A. Dlg-1 Interacts With and Regulates the Activities of Fibroblast Growth Factor Receptors and EphA2 in the Mouse Lens. Invest Ophthalmol Vis Sci. 2016;57:707-18 pubmed publisher
western blot; human; fig s2	Bleckmann A, Conradi L, Menck K, Schmick N, Schubert A, RietkÃ¶tter E, et al. Î²-catenin-independent WNT signaling and Ki67 in contrast to the estrogen receptor status are prognostic and associated with poor prognosis in breast cancer liver metastases. Clin Exp Metastasis. 2016;33:309-23 pubmed publisher
immunohistochemistry; mouse; 100 ng/ml; loading ...; fig 9b	Wymeersch F, Huang Y, Blin G, Cambray N, Wilkie R, Wong F, et al. Position-dependent plasticity of distinct progenitor types in the primitive streak. elife. 2016;5:e10042 pubmed publisher
western blot; human; 1:300; fig 3	Ogaki S, Morooka M, Otera K, Kume S. A cost-effective system for differentiation of intestinal epithelium from human induced pluripotent stem cells. Sci Rep. 2015;5:17297 pubmed publisher
immunocytochemistry; human; 1:1000; fig 3	Hudson B, Hum N, Thomas C, Kohlgruber A, Sebastian A, Collette N, et al. SOST Inhibits Prostate Cancer Invasion. PLoS ONE. 2015;10:e0142058 pubmed publisher
immunocytochemistry; human; 1:50 western blot; human; fig 5	Sellerio A, Ciusani E, Ben Moshe N, Coco S, Piccinini A, Myers C, et al. Overshoot during phenotypic switching of cancer cell populations. Sci Rep. 2015;5:15464 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:1000; loading ...; fig 4a	Kim S, Bozeman R, Kaisani A, Kim W, Zhang L, Richardson J, et al. Radiation promotes colorectal cancer initiation and progression by inducing senescence-associated inflammatory responses. Oncogene. 2016;35:3365-75 pubmed publisher
western blot; human; 1:500; loading ...; fig s8a	Mughal A, Grieg Z, Skjellegrind H, Fayzullin A, Lamkhannat M, Joel M, et al. Knockdown of NAT12/NAA30 reduces tumorigenic features of glioblastoma-initiating cells. Mol Cancer. 2015;14:160 pubmed publisher
immunohistochemistry - paraffin section; human; 1:50	McBryan J, Fagan A, McCartan D, Bane F, VareÅ¡lija D, Cocchiglia S, et al. Transcriptomic Profiling of Sequential Tumors from Breast Cancer Patients Provides a Global View of Metastatic Expression Changes Following Endocrine Therapy. Clin Cancer Res. 2015;21:5371-9 pubmed publisher
immunoprecipitation; human immunohistochemistry; human	Burkhalter R, Westfall S, Liu Y, Stack M. Lysophosphatidic Acid Initiates Epithelial to Mesenchymal Transition and Induces β-Catenin-mediated Transcription in Epithelial Ovarian Carcinoma. J Biol Chem. 2015;290:22143-54 pubmed publisher
immunocytochemistry; human; loading ...; fig 6d western blot; human; loading ...; fig 6c	Ayadi M, Bouygues A, Ouaret D, Ferrand N, Chouaib S, Thiery J, et al. Chronic chemotherapeutic stress promotes evolution of stemness and WNT/beta-catenin signaling in colorectal cancer cells: implications for clinical use of WNT-signaling inhibitors. Oncotarget. 2015;6:18518-33 pubmed
western blot; human; 1:1000; fig 1c	Zhu D, Wang Z, Zhao J, Calimeri T, Meng J, Hideshima T, et al. The Cyclophilin A-CD147 complex promotes the proliferation and homing of multiple myeloma cells. Nat Med. 2015;21:572-80 pubmed publisher
western blot; human; fig 5 immunohistochemistry - paraffin section; mouse; fig 4	Cheng Y, Phoon Y, Jin X, Chong S, Ip J, Wong B, et al. Wnt-C59 arrests stemness and suppresses growth of nasopharyngeal carcinoma in mice by inhibiting the Wnt pathway in the tumor microenvironment. Oncotarget. 2015;6:14428-39 pubmed
western blot; mouse; 1:2000; fig 6	Laurent A, Calabrese M, Warnatz H, Yaspo M, Tkachuk V, Torres M, et al. ChIP-Seq and RNA-Seq analyses identify components of the Wnt and Fgf signaling pathways as Prep1 target genes in mouse embryonic stem cells. PLoS ONE. 2015;10:e0122518 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:500	Sakamaki A, Katsuragi Y, Otsuka K, Tomita M, Obata M, Iwasaki T, et al. Bcl11b SWI/SNF-complex subunit modulates intestinal adenoma and regeneration after Î³-irradiation through Wnt/Î²-catenin pathway. Carcinogenesis. 2015;36:622-31 pubmed publisher
western blot; human; loading ...; fig 2d	ÄŒajÃ¡nek L, Glatter T, Nigg E. The E3 ubiquitin ligase Mib1 regulates Plk4 and centriole biogenesis. J Cell Sci. 2015;128:1674-82 pubmed publisher
other; human; loading ...; fig 3a	Traenkle B, Emele F, Anton R, Poetz O, Haeussler R, Maier J, et al. Monitoring interactions and dynamics of endogenous beta-catenin with intracellular nanobodies in living cells. Mol Cell Proteomics. 2015;14:707-23 pubmed publisher
immunohistochemistry - paraffin section; mouse western blot; mouse	Bisson J, Mills B, Paul Helt J, Zwaka T, Cohen E. Wnt5a and Wnt11 inhibit the canonical Wnt pathway and promote cardiac progenitor development via the Caspase-dependent degradation of AKT. Dev Biol. 2015;398:80-96 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:200; fig 4i	Yuan G, Yang G, Zheng Y, Zhu X, Chen Z, Zhang Z, et al. The non-canonical BMP and Wnt/β-catenin signaling pathways orchestrate early tooth development. Development. 2015;142:128-39 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100	Isobe T, Hisamori S, Hogan D, Zabala M, Hendrickson D, Dalerba P, et al. miR-142 regulates the tumorigenicity of human breast cancer stem cells through the canonical WNT signaling pathway. elife. 2014;3: pubmed publisher
western blot; mouse; 1:1000; fig 4	Easter S, Mitchell E, Baxley S, Desmond R, Frost A, Serra R. Wnt5a suppresses tumor formation and redirects tumor phenotype in MMTV-Wnt1 tumors. PLoS ONE. 2014;9:e113247 pubmed publisher
western blot; human	Kim K, Ossipova O, Sokol S. Neural crest specification by inhibition of the ROCK/Myosin II pathway. Stem Cells. 2015;33:674-85 pubmed publisher
immunocytochemistry; human; 1:100 western blot; human; 1:1000	Joseph J, Conroy S, Tomar T, Eggens Meijer E, Bhat K, Copray S, et al. TGF-Î² is an inducer of ZEB1-dependent mesenchymal transdifferentiation in glioblastoma that is associated with tumor invasion. Cell Death Dis. 2014;5:e1443 pubmed publisher
western blot; human; 1:1000; loading ...; fig 5a	Jansen S, Holman R, Hedemann I, Frankes E, Elzinga C, Timens W, et al. Prostaglandin E2 promotes MYCN non-amplified neuroblastoma cell survival via Î²-catenin stabilization. J Cell Mol Med. 2015;19:210-26 pubmed publisher
western blot; human	Jia D, Yang W, Li L, Liu H, Tan Y, Ooi S, et al. Î²-Catenin and NF-ÎºB co-activation triggered by TLR3 stimulation facilitates stem cell-like phenotypes in breast cancer. Cell Death Differ. 2015;22:298-310 pubmed publisher
western blot; mouse; 1:2500	Forsdahl S, Kiselev Y, Hogseth R, Mjelle J, Mikkola I. Pax6 regulates the expression of Dkk3 in murine and human cell lines, and altered responses to Wnt signaling are shown in FlpIn-3T3 cells stably expressing either the Pax6 or the Pax6(5a) isoform. PLoS ONE. 2014;9:e102559 pubmed publisher
western blot; mouse	Etnyre D, Stone A, Fong J, Jacobs R, Uppada S, Botting G, et al. Targeting c-Met in melanoma: mechanism of resistance and efficacy of novel combinatorial inhibitor therapy. Cancer Biol Ther. 2014;15:1129-41 pubmed publisher
flow cytometry; human; fig 7g	Lu T, Aron L, Zullo J, Pan Y, Kim H, Chen Y, et al. REST and stress resistance in ageing and Alzheimer's disease. Nature. 2014;507:448-54 pubmed publisher
immunocytochemistry; mouse western blot; mouse	Wang H, Sun W, Ma J, Pan Y, Wang L, Zhang W. Polycystin-1 mediates mechanical strain-induced osteoblastic mechanoresponses via potentiation of intracellular calcium and Akt/?-catenin pathway. PLoS ONE. 2014;9:e91730 pubmed publisher
western blot; human	Guezguez A, Paré F, Benoit Y, Basora N, Beaulieu J. Modulation of stemness in a human normal intestinal epithelial crypt cell line by activation of the WNT signaling pathway. Exp Cell Res. 2014;322:355-64 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:50	Yukata K, Xie C, Li T, Takahata M, Hoak D, Kondabolu S, et al. Aging periosteal progenitor cells have reduced regenerative responsiveness to bone injury and to the anabolic actions of PTH 1-34 treatment. Bone. 2014;62:79-89 pubmed publisher
western blot; human	Svedlund J, Barazeghi E, Stalberg P, Hellman P, Akerstrom G, Björklund P, et al. The histone methyltransferase EZH2, an oncogene common to benign and malignant parathyroid tumors. Endocr Relat Cancer. 2014;21:231-9 pubmed publisher
immunocytochemistry; mouse; 1:200 western blot; mouse; 1:2000	Naito M, Mikami Y, Takagi M, Takahashi T. Up-regulation of Axin2 by dexamethasone promotes adipocyte differentiation in ROB-C26 mesenchymal progenitor cells. Cell Tissue Res. 2013;354:761-70 pubmed publisher
western blot; human; 1:400	Luijten M, Basten S, Claessens T, Vernooij M, Scott C, Janssen R, et al. Birt-Hogg-Dube syndrome is a novel ciliopathy. Hum Mol Genet. 2013;22:4383-97 pubmed publisher
chromatin immunoprecipitation; human immunoprecipitation; human immunocytochemistry; human western blot; human	Yu Y, Qi L, Wu J, Wang Y, Fang W, Zhang H. Kindlin 2 regulates myogenic related factor myogenin via a canonical Wnt signaling in myogenic differentiation. PLoS ONE. 2013;8:e63490 pubmed publisher
western blot; human; 1:2000; fig 1f	Li Y, Zheng Y, Izumi K, Ishiguro H, Ye B, Li F, et al. Androgen activates ?-catenin signaling in bladder cancer cells. Endocr Relat Cancer. 2013;20:293-304 pubmed publisher
western blot; human	Han J, Soletti R, Sadarangani A, Sridevi P, Ramirez M, Eckmann L, et al. Nuclear expression of ?-catenin promotes RB stability and resistance to TNF-induced apoptosis in colon cancer cells. Mol Cancer Res. 2013;11:207-18 pubmed publisher
western blot; human	Thu K, Radulovich N, Becker Santos D, Pikor L, Pusic A, Lockwood W, et al. SOX15 is a candidate tumor suppressor in pancreatic cancer with a potential role in Wnt/?-catenin signaling. Oncogene. 2014;33:279-88 pubmed publisher
western blot; mouse	Styner M, Meyer M, Galior K, Case N, Xie Z, Sen B, et al. Mechanical strain downregulates C/EBP? in MSC and decreases endoplasmic reticulum stress. PLoS ONE. 2012;7:e51613 pubmed publisher
immunohistochemistry - paraffin section; human	Urdinguio R, Fernandez A, Moncada Pazos A, Huidobro C, Rodriguez R, Ferrero C, et al. Immune-dependent and independent antitumor activity of GM-CSF aberrantly expressed by mouse and human colorectal tumors. Cancer Res. 2013;73:395-405 pubmed publisher
western blot; human; 2 ug/ml	McClain C, Sim F, Goldman S. Pleiotrophin suppression of receptor protein tyrosine phosphatase-?/? maintains the self-renewal competence of fetal human oligodendrocyte progenitor cells. J Neurosci. 2012;32:15066-75 pubmed publisher
immunocytochemistry; human; 1:150 western blot; human; 1:1000	Skalka N, Caspi M, Caspi E, Loh Y, Rosin Arbesfeld R. Carboxypeptidase E: a negative regulator of the canonical Wnt signaling pathway. Oncogene. 2013;32:2836-47 pubmed publisher
western blot; mouse; 1:1000; fig 4	MARCIANO D, BRAKEMAN P, Lee C, Spivak N, Eastburn D, Bryant D, et al. p120 catenin is required for normal renal tubulogenesis and glomerulogenesis. Development. 2011;138:2099-109 pubmed publisher
immunohistochemistry - paraffin section; mouse western blot; mouse	Li J, Swope D, Raess N, Cheng L, Muller E, Radice G. Cardiac tissue-restricted deletion of plakoglobin results in progressive cardiomyopathy and activation of {beta}-catenin signaling. Mol Cell Biol. 2011;31:1134-44 pubmed publisher
ELISA; rhesus macaque; 1:300; fig 2	Cheng X, Huber T, Chen V, Gadue P, Keller G. Numb mediates the interaction between Wnt and Notch to modulate primitive erythropoietic specification from the hemangioblast. Development. 2008;135:3447-58 pubmed publisher
western blot; human	Ray S, Xu F, Wang H, Das S. Cooperative control via lymphoid enhancer factor 1/T cell factor 3 and estrogen receptor-alpha for uterine gene regulation by estrogen. Mol Endocrinol. 2008;22:1125-40 pubmed publisher
	Chen Y, Kang X, Zhou Z, Yang J, Xin Q, Ying C, et al. MiR-1908/EXO1 and MiR-203a/FOS, regulated by scd1, are associated with fracture risk and bone health in postmenopausal diabetic women. Aging (Albany NY). 2020;12:9549-9584 pubmed publisher
	Park J, Kim D, Shah S, Kim H, Kshitiz -, Kim P, et al. Switch-like enhancement of epithelial-mesenchymal transition by YAP through feedback regulation of WT1 and Rho-family GTPases. Nat Commun. 2019;10:2797 pubmed publisher
	Antas P, Novellasdemunt L, Kucharska A, Massie I, Carvalho J, Oukrif D, et al. SH3BP4 Regulates Intestinal Stem Cells and Tumorigenesis by Modulating β-Catenin Nuclear Localization. Cell Rep. 2019;26:2266-2273.e4 pubmed publisher
	Samarajeewa A, Lenz D, Xie L, Chiang H, Kirchner R, Mulvaney J, et al. Transcriptional response to Wnt activation regulates the regenerative capacity of the mammalian cochlea. Development. 2018;145: pubmed publisher
	Dong P, Xiong Y, Yu J, Chen L, Tao T, Yi S, et al. Control of PD-L1 expression by miR-140/142/340/383 and oncogenic activation of the OCT4-miR-18a pathway in cervical cancer. Oncogene. 2018;37:5257-5268 pubmed publisher
	Nayak G, Odaka Y, Prasad V, Solano A, Yeo E, Vemaraju S, et al. Developmental vascular regression is regulated by a Wnt/β-catenin, MYC and CDKN1A pathway that controls cell proliferation and cell death. Development. 2018;145: pubmed publisher
	Griffin J, del Viso F, Duncan A, Robson A, Hwang W, Kulkarni S, et al. RAPGEF5 Regulates Nuclear Translocation of β-Catenin. Dev Cell. 2018;44:248-260.e4 pubmed publisher
	Wu M, Wang Y, Shao J, Wang J, Chen W, Li Y. Cbfβ governs osteoblast-adipocyte lineage commitment through enhancing β-catenin signaling and suppressing adipogenesis gene expression. Proc Natl Acad Sci U S A. 2017;114:10119-10124 pubmed publisher
	Thiery A, Shono T, Kurokawa D, Britz R, Johanson Z, Fraser G. Spatially restricted dental regeneration drives pufferfish beak development. Proc Natl Acad Sci U S A. 2017;114:E4425-E4434 pubmed publisher
	Zhang H, Hui H, Li Z, Pan J, Jiang X, Wei T, et al. Pigment epithelium-derived factor attenuates myocardial fibrosis via inhibiting Endothelial-to-Mesenchymal Transition in rats with acute myocardial infarction. Sci Rep. 2017;7:41932 pubmed publisher
	Wu V, Wang S, Chueh S, Yang S, Huang K, Lin Y, et al. The prevalence of CTNNB1 mutations in primary aldosteronism and consequences for clinical outcomes. Sci Rep. 2017;7:39121 pubmed publisher
	Qi Z, Li Y, Zhao B, Xu C, Liu Y, Li H, et al. BMP restricts stemness of intestinal Lgr5+ stem cells by directly suppressing their signature genes. Nat Commun. 2017;8:13824 pubmed publisher
	Renner G, Noulet F, Mercier M, Choulier L, Etienne Selloum N, Gies J, et al. Expression/activation of ?5?1 integrin is linked to the ?-catenin signaling pathway to drive migration in glioma cells. Oncotarget. 2016;7:62194-62207 pubmed publisher
	Pratheeshkumar P, Son Y, Divya S, Turcios L, Roy R, Hitron J, et al. Hexavalent chromium induces malignant transformation of human lung bronchial epithelial cells via ROS-dependent activation of miR-21-PDCD4 signaling. Oncotarget. 2016;7:51193-51210 pubmed publisher
	AlÃ©n B, Leal LÃ³pez S, AlÃ©n M, ViaÃ±o P, GarcÃa Castro V, Mosteiro C, et al. The role of the obestatin/GPR39 system in human gastric adenocarcinomas. Oncotarget. 2016;7:5957-71 pubmed publisher
	Telias M, Mayshar Y, Amit A, Ben Yosef D. Molecular mechanisms regulating impaired neurogenesis of fragile X syndrome human embryonic stem cells. Stem Cells Dev. 2015;24:2353-65 pubmed publisher
	Schlupf J, Steinbeisser H. IGF antagonizes the Wnt/Î²-Catenin pathway and promotes differentiation of extra-embryonic endoderm. Differentiation. 2014;87:209-19 pubmed publisher
	Bhushan B, Walesky C, Manley M, Gallagher T, Borude P, Edwards G, et al. Pro-regenerative signaling after acetaminophen-induced acute liver injury in mice identified using a novel incremental dose model. Am J Pathol. 2014;184:3013-25 pubmed publisher
	Antony L, van der Schoor F, Dalrymple S, Isaacs J. Androgen receptor (AR) suppresses normal human prostate epithelial cell proliferation via AR/?-catenin/TCF-4 complex inhibition of c-MYC transcription. Prostate. 2014;74:1118-31 pubmed publisher
	Sun T, Co N, Wong N. PFTK1 interacts with cyclin Y to activate non-canonical Wnt signaling in hepatocellular carcinoma. Biochem Biophys Res Commun. 2014;449:163-8 pubmed publisher
	Zahoor M, Cha P, Choi K. Indirubin-3'-oxime, an activator of Wnt/?-catenin signaling, enhances osteogenic commitment of ST2 cells and restores bone loss in high-fat diet-induced obese male mice. Bone. 2014;65:60-8 pubmed publisher
	Kang W, Tong J, Chan A, Zhao J, Dong Y, Wang S, et al. Yin Yang 1 contributes to gastric carcinogenesis and its nuclear expression correlates with shorter survival in patients with early stage gastric adenocarcinoma. J Transl Med. 2014;12:80 pubmed publisher
	Umazume K, Tsukahara R, Liu L, Fernandez de Castro J, McDonald K, Kaplan H, et al. Role of retinal pigment epithelial cell ?-catenin signaling in experimental proliferative vitreoretinopathy. Am J Pathol. 2014;184:1419-28 pubmed publisher
	Xie Q, Chen L, Shan X, Shan X, Tang J, Zhou F, et al. Epigenetic silencing of SFRP1 and SFRP5 by hepatitis B virus X protein enhances hepatoma cell tumorigenicity through Wnt signaling pathway. Int J Cancer. 2014;135:635-46 pubmed publisher
	Li C, Zhang S, Lu Y, Zhang Y, Wang E, Cui Z. The roles of Notch3 on the cell proliferation and apoptosis induced by CHIR99021 in NSCLC cell lines: a functional link between Wnt and Notch signaling pathways. PLoS ONE. 2013;8:e84659 pubmed publisher
	Fong J, Jacobs R, Moravec D, Uppada S, Botting G, Nlend M, et al. Alternative signaling pathways as potential therapeutic targets for overcoming EGFR and c-Met inhibitor resistance in non-small cell lung cancer. PLoS ONE. 2013;8:e78398 pubmed publisher
	Bilir B, Kucuk O, Moreno C. Wnt signaling blockage inhibits cell proliferation and migration, and induces apoptosis in triple-negative breast cancer cells. J Transl Med. 2013;11:280 pubmed publisher
	Li L, Ying J, Tong X, Zhong L, Su X, Xiang T, et al. Epigenetic identification of receptor tyrosine kinase-like orphan receptor 2 as a functional tumor suppressor inhibiting ?-catenin and AKT signaling but frequently methylated in common carcinomas. Cell Mol Life Sci. 2014;71:2179-92 pubmed publisher
	Ghazvini M, Sonneveld P, Kremer A, Franken P, Sacchetti A, Atlasi Y, et al. Cancer stemness in Apc- vs. Apc/KRAS-driven intestinal tumorigenesis. PLoS ONE. 2013;8:e73872 pubmed publisher
	Miyazaki T, Miyazaki S, Ashida M, Tanaka T, Tashiro F, Miyazaki J. Functional analysis of Tcl1 using Tcl1-deficient mouse embryonic stem cells. PLoS ONE. 2013;8:e71645 pubmed publisher
	Tiwari A, Schneider M, Fiorino A, Haider R, Okoniewski M, Roschitzki B, et al. Early insights into the function of KIAA1199, a markedly overexpressed protein in human colorectal tumors. PLoS ONE. 2013;8:e69473 pubmed publisher
	Engert S, Burtscher I, Liao W, Dulev S, Schotta G, Lickert H. Wnt/?-catenin signalling regulates Sox17 expression and is essential for organizer and endoderm formation in the mouse. Development. 2013;140:3128-38 pubmed publisher
	Tang Z, Dong Q, Cui Q, Papavassiliou P, Wang E, Wang E. Ataxia-telangiectasia group D complementing gene (ATDC) promotes lung cancer cell proliferation by activating NF-?B pathway. PLoS ONE. 2013;8:e63676 pubmed publisher
	Son Y, Pratheeshkumar P, Wang L, Wang X, Fan J, Kim D, et al. Reactive oxygen species mediate Cr(VI)-induced carcinogenesis through PI3K/AKT-dependent activation of GSK-3?/?-catenin signaling. Toxicol Appl Pharmacol. 2013;271:239-48 pubmed publisher
	Shao L, Oshima S, Duong B, Advincula R, Barrera J, Malynn B, et al. A20 restricts wnt signaling in intestinal epithelial cells and suppresses colon carcinogenesis. PLoS ONE. 2013;8:e62223 pubmed publisher
	Ludwig K, Tse E, Wang J. Colon cancer cells adopt an invasive phenotype without mesenchymal transition in 3-D but not 2-D culture upon combined stimulation with EGF and crypt growth factors. BMC Cancer. 2013;13:221 pubmed publisher
	Wang Y, Huang C, Reddy Chintagari N, Bhaskaran M, Weng T, Guo Y, et al. miR-375 regulates rat alveolar epithelial cell trans-differentiation by inhibiting Wnt/?-catenin pathway. Nucleic Acids Res. 2013;41:3833-44 pubmed publisher
	Chen J, Chan A, To K, Chen W, Zhang Z, Ren J, et al. SIRT2 overexpression in hepatocellular carcinoma mediates epithelial to mesenchymal transition by protein kinase B/glycogen synthase kinase-3?/?-catenin signaling. Hepatology. 2013;57:2287-98 pubmed publisher
	Latasa M, Salis F, Urtasun R, Garcia Irigoyen O, Elizalde M, Uriarte I, et al. Regulation of amphiregulin gene expression by ?-catenin signaling in human hepatocellular carcinoma cells: a novel crosstalk between FGF19 and the EGFR system. PLoS ONE. 2012;7:e52711 pubmed publisher
	Li Q, Kannan A, Das A, Demayo F, Hornsby P, Young S, et al. WNT4 acts downstream of BMP2 and functions via ?-catenin signaling pathway to regulate human endometrial stromal cell differentiation. Endocrinology. 2013;154:446-57 pubmed publisher
	Liu L, Alonso V, Guo L, Tourkova I, Henderson S, Almarza A, et al. Na+/H+ exchanger regulatory factor 1 (NHERF1) directly regulates osteogenesis. J Biol Chem. 2012;287:43312-21 pubmed publisher
	Wei W, Sun H, Ting K, Zhang L, Lee H, Li G, et al. Inhibition of cardiomyocytes differentiation of mouse embryonic stem cells by CD38/cADPR/Ca2+ signaling pathway. J Biol Chem. 2012;287:35599-611 pubmed publisher
	Son Y, Wang L, Poyil P, Budhraja A, Hitron J, Zhang Z, et al. Cadmium induces carcinogenesis in BEAS-2B cells through ROS-dependent activation of PI3K/AKT/GSK-3?/?-catenin signaling. Toxicol Appl Pharmacol. 2012;264:153-60 pubmed publisher
	Tran T, Shi X, Zaia J, Ai X. Heparan sulfate 6-O-endosulfatases (Sulfs) coordinate the Wnt signaling pathways to regulate myoblast fusion during skeletal muscle regeneration. J Biol Chem. 2012;287:32651-64 pubmed
	Shi Y, Yuan S, Li B, Wang J, Carlton S, Chung K, et al. Regulation of Wnt signaling by nociceptive input in animal models. Mol Pain. 2012;8:47 pubmed publisher
	Ng F, Toh T, Ting E, Koh G, Sandanaraj E, Phong M, et al. Progenitor-like traits contribute to patient survival and prognosis in oligodendroglial tumors. Clin Cancer Res. 2012;18:4122-35 pubmed
	Clinkenbeard E, Butler J, Spear B. Pericentral activity of alpha-fetoprotein enhancer 3 and glutamine synthetase upstream enhancer in the adult liver are regulated by ?-catenin in mice. Hepatology. 2012;56:1892-901 pubmed publisher
	Kohan Ghadr H, Smith L, Arnold D, Murphy B, Lefebvre R. Aberrant expression of E-cadherin and ?-catenin proteins in placenta of bovine embryos derived from somatic cell nuclear transfer. Reprod Fertil Dev. 2012;24:588-98 pubmed publisher
	Law P, Ching A, Chan A, Wong Q, Wong C, To K, et al. MiR-145 modulates multiple components of the insulin-like growth factor pathway in hepatocellular carcinoma. Carcinogenesis. 2012;33:1134-41 pubmed publisher
	Vinuesa A, Sancho R, García Limones C, Behrens A, Ten Dijke P, Calzado M, et al. Vanilloid receptor-1 regulates neurogenic inflammation in colon and protects mice from colon cancer. Cancer Res. 2012;72:1705-16 pubmed publisher
	Pourreyron C, Reilly L, Proby C, Panteleyev A, Fleming C, McLean K, et al. Wnt5a is strongly expressed at the leading edge in non-melanoma skin cancer, forming active gradients, while canonical Wnt signalling is repressed. PLoS ONE. 2012;7:e31827 pubmed publisher
	Jacob F, Ukegjini K, Nixdorf S, Ford C, Olivier J, Caduff R, et al. Loss of secreted frizzled-related protein 4 correlates with an aggressive phenotype and predicts poor outcome in ovarian cancer patients. PLoS ONE. 2012;7:e31885 pubmed publisher
	Yoshioka S, King M, Ran S, Okuda H, Maclean J, McAsey M, et al. WNT7A regulates tumor growth and progression in ovarian cancer through the WNT/β-catenin pathway. Mol Cancer Res. 2012;10:469-82 pubmed publisher
	Li L, Ying J, Li H, Zhang Y, Shu X, Fan Y, et al. The human cadherin 11 is a pro-apoptotic tumor suppressor modulating cell stemness through Wnt/?-catenin signaling and silenced in common carcinomas. Oncogene. 2012;31:3901-12 pubmed publisher
	Kodithuwakku S, Pang R, Ng E, Cheung A, Horne A, Ho P, et al. Wnt activation downregulates olfactomedin-1 in Fallopian tubal epithelial cells: a microenvironment predisposed to tubal ectopic pregnancy. Lab Invest. 2012;92:256-64 pubmed publisher
	Ceballos M, Parody J, Alvarez M, Ingaramo P, Carnovale C, Carrillo M. Interferon-?2b and transforming growth factor-?1 treatments on HCC cell lines: Are Wnt/?-catenin pathway and Smads signaling connected in hepatocellular carcinoma?. Biochem Pharmacol. 2011;82:1682-91 pubmed publisher
	Wang D, Dai C, Li Y, Liu Y. Canonical Wnt/?-catenin signaling mediates transforming growth factor-?1-driven podocyte injury and proteinuria. Kidney Int. 2011;80:1159-1169 pubmed publisher
	Luan L, Ding T, Stinnett A, Reese J, Paria B. Adherens junction proteins in the hamster uterus: their contributions to the success of implantation. Biol Reprod. 2011;85:996-1004 pubmed publisher
	Kato H, Gruenwald A, Suh J, Miner J, Barisoni Thomas L, Taketo M, et al. Wnt/?-catenin pathway in podocytes integrates cell adhesion, differentiation, and survival. J Biol Chem. 2011;286:26003-15 pubmed publisher
	Liu J, Wang Z, Tang J, Tang R, Shan X, Zhang W, et al. Hepatitis C virus core protein activates Wnt/?-catenin signaling through multiple regulation of upstream molecules in the SMMC-7721 cell line. Arch Virol. 2011;156:1013-23 pubmed publisher
	Mödder U, Oursler M, Khosla S, Monroe D. Wnt10b activates the Wnt, notch, and NF?B pathways in U2OS osteosarcoma cells. J Cell Biochem. 2011;112:1392-402 pubmed publisher
	Griffin C, Curtis C, Davis R, Muthukumar V, Magnuson T. The chromatin-remodeling enzyme BRG1 modulates vascular Wnt signaling at two levels. Proc Natl Acad Sci U S A. 2011;108:2282-7 pubmed publisher
	Blanchart A, Martín López E, de Carlos J, López Mascaraque L. Peripheral contributions to olfactory bulb cell populations (migrations towards the olfactory bulb). Glia. 2011;59:278-92 pubmed publisher
	Lussier C, Brial F, Roy S, Langlois M, Verdu E, Rivard N, et al. Loss of hepatocyte-nuclear-factor-1alpha impacts on adult mouse intestinal epithelial cell growth and cell lineages differentiation. PLoS ONE. 2010;5:e12378 pubmed publisher
	Winslow B, Burke A. Atypical molecular profile for joint development in the avian costal joint. Dev Dyn. 2010;239:2547-57 pubmed publisher
	Behr B, Longaker M, Quarto N. Differential activation of canonical Wnt signaling determines cranial sutures fate: a novel mechanism for sagittal suture craniosynostosis. Dev Biol. 2010;344:922-40 pubmed publisher
	Bosco E, Nakai Y, Hennigan R, Ratner N, Zheng Y. NF2-deficient cells depend on the Rac1-canonical Wnt signaling pathway to promote the loss of contact inhibition of proliferation. Oncogene. 2010;29:2540-9 pubmed publisher
	Lambertini E, Lisignoli G, Torreggiani E, Manferdini C, Gabusi E, Franceschetti T, et al. Slug gene expression supports human osteoblast maturation. Cell Mol Life Sci. 2009;66:3641-53 pubmed publisher
	Warr N, Siggers P, Bogani D, Brixey R, Pastorelli L, Yates L, et al. Sfrp1 and Sfrp2 are required for normal male sexual development in mice. Dev Biol. 2009;326:273-84 pubmed publisher
	Björklund P, Lindberg D, Akerstrom G, Westin G. Stabilizing mutation of CTNNB1/beta-catenin and protein accumulation analyzed in a large series of parathyroid tumors of Swedish patients. Mol Cancer. 2008;7:53 pubmed publisher
	Jia L, Miao C, Cao Y, Duan E. Effects of Wnt proteins on cell proliferation and apoptosis in HEK293 cells. Cell Biol Int. 2008;32:807-13 pubmed publisher
	Welters H, Oknianska A, Erdmann K, Ryffel G, Morgan N. The protein tyrosine phosphatase-BL, modulates pancreatic beta-cell proliferation by interaction with the Wnt signalling pathway. J Endocrinol. 2008;197:543-52 pubmed publisher
	Espada J, Varela I, Flores I, Ugalde A, Cadinanos J, Pendas A, et al. Nuclear envelope defects cause stem cell dysfunction in premature-aging mice. J Cell Biol. 2008;181:27-35 pubmed publisher
	Björklund P, Akerstrom G, Westin G. An LRP5 receptor with internal deletion in hyperparathyroid tumors with implications for deregulated WNT/beta-catenin signaling. PLoS Med. 2007;4:e328 pubmed

product information

Catalog Number :

05-665

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Active-β-Catenin (Anti-ABC) Antibody, clone 8E7

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P35222

Host Name :

Mouse

Antigen :

Active-β-Catenin (ABC)

Clone :

8E7

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-Active-β-Catenin (Anti-ABC) Antibody, clone 8E7

Cross Reactivity :

Human;Mouse;Rat

Background :

Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as α-catenin and β-catenin. α-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. When β-catenin was sequenced it was found to be a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain which is specialized for protein-protein binding. An increase in β-catenin production has been noted in those people who have Basal Cell Carcinoma and leads to the increase in proliferation of related tumors. When β-catenin is not associated with cadherins and α-catenin, it can interact with other proteins such as Catenin Beta Interacting Protein 1 (ICAT) and adenomatosis polyposis coli (APC). Recent evidence suggests that β-catenin plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.

Immunogen :

Fusion protein corresponding to residues 1-100 of human β-catenin with a c-terminal histidine tag

Specificity :

Recognizes active form of β-catenin, dephosphorylated on Ser37 or Thr41.

Package Size :

100 μg

Uses :

Flow Cytometry;Immunocytochemistry;Immunohistochemistry;Immunohistochemistry (Paraffin);Western Blotting

Storage :

Maintain for 1 year at 2-8°C from date of shipment. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.