Bmi-1 Antibody, clone F6 | EMD Millipore 05-637 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Bmi-1 Antibody, clone F6

catalog :

05-637

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section

citations: 39

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig 2a	Ricci B, Millner T, Pomella N, Zhang X, Guglielmi L, Badodi S, et al. Polycomb-mediated repression of EphrinA5 promotes growth and invasion of glioblastoma. Oncogene. 2020;39:2523-2538 pubmed publisher
immunohistochemistry - paraffin section; human; loading ...; fig 2d, 6d western blot; human; loading ...; fig 3a, 3d	Aldaz P, Otaegi Ugartemendia M, Sáenz Antoñanzas A, Garcia Puga M, Moreno Valladares M, Flores J, et al. SOX9 promotes tumor progression through the axis BMI1-p21CIP. Sci Rep. 2020;10:357 pubmed publisher
western blot; human; 1:1000; loading ...; fig 1s1a	Azkanaz M, Rodríguez López A, de Boer B, Huiting W, Angrand P, Vellenga E, et al. Protein quality control in the nucleolus safeguards recovery of epigenetic regulators after heat shock. elife. 2019;8: pubmed publisher
western blot; mouse; loading ...; fig 2j	Chen L, Yang R, Qiao W, Zhang W, Chen J, Mao L, et al. 1,25-Dihydroxyvitamin D exerts an antiaging role by activation of Nrf2-antioxidant signaling and inactivation of p16/p53-senescence signaling. Aging Cell. 2019;18:e12951 pubmed publisher
western blot; human; 1:1000; loading ...; fig 4	Paschos K, Bazot Q, Lees J, Farrell P, Allday M. Requirement for PRC1 subunit BMI1 in host gene activation by Epstein-Barr virus protein EBNA3C. Nucleic Acids Res. 2019;47:2807-2821 pubmed publisher
immunocytochemistry; human; 1:1000; loading ...; fig 7f	Patel N, Garikapati K, Pandita R, Singh D, Pandita T, Bhadra U, et al. miR-15a/miR-16 down-regulates BMI1, impacting Ub-H2A mediated DNA repair and breast cancer cell sensitivity to doxorubicin. Sci Rep. 2017;7:4263 pubmed publisher
western blot; human; 1:500	Britschgi A, Duss S, Kim S, Couto J, Brinkhaus H, Koren S, et al. The Hippo kinases LATS1 and 2 control human breast cell fate via crosstalk with ERα. Nature. 2017;541:541-545 pubmed publisher
immunohistochemistry - paraffin section; human; fig 2	Munthe S, Petterson S, Dahlrot R, Poulsen F, Hansen S, Kristensen B. Glioma Cells in the Tumor Periphery Have a Stem Cell Phenotype. PLoS ONE. 2016;11:e0155106 pubmed publisher
immunohistochemistry; mouse; 1:100; fig 1 western blot; mouse; 1:500; fig 2	Benitz S, Regel I, Reinhard T, Popp A, SchÃ¤ffer I, Raulefs S, et al. Polycomb repressor complex 1 promotes gene silencing through H2AK119 mono-ubiquitination in acinar-to-ductal metaplasia and pancreatic cancer cells. Oncotarget. 2016;7:11424-33 pubmed publisher
immunohistochemistry - paraffin section; human; 1:50	Robl B, Pauli C, Botter S, Bode Lesniewska B, Fuchs B. Prognostic value of tumor suppressors in osteosarcoma before and after neoadjuvant chemotherapy. BMC Cancer. 2015;15:379 pubmed publisher
chromatin immunoprecipitation; mouse western blot; mouse; 1:1000; fig 3a	Pradeepa M, Grimes G, Taylor G, Sutherland H, Bickmore W. Psip1/Ledgf p75 restrains Hox gene expression by recruiting both trithorax and polycomb group proteins. Nucleic Acids Res. 2014;42:9021-32 pubmed publisher
western blot; human; 1:500	Jung Y, Vermeer P, Vermeer D, Lee S, Goh A, Ahn H, et al. CD200: association with cancer stem cell features and response to chemoradiation in head and neck squamous cell carcinoma. Head Neck. 2015;37:327-35 pubmed publisher
chromatin immunoprecipitation; human immunoprecipitation; human; 1:500 western blot; human; 1:500	Cao Q, Wang X, Zhao M, Yang R, Malik R, Qiao Y, et al. The central role of EED in the orchestration of polycomb group complexes. Nat Commun. 2014;5:3127 pubmed publisher
chromatin immunoprecipitation; mouse chromatin immunoprecipitation; human	Zhou J, Dhawan S, Fu H, Snyder E, Bottino R, Kundu S, et al. Combined modulation of polycomb and trithorax genes rejuvenates ? cell replication. J Clin Invest. 2013;123:4849-58 pubmed
immunocytochemistry; mouse	Legartova S, Stixova L, Laur O, Kozubek S, Sehnalová P, Bartova E. Nuclear structures surrounding internal lamin invaginations. J Cell Biochem. 2014;115:476-87 pubmed publisher
immunohistochemistry - paraffin section; human; 1:150	Ho T, Chen S, Wu J, Ho M, Chen L, Hsieh J, et al. PEDF promotes self-renewal of limbal stem cell and accelerates corneal epithelial wound healing. Stem Cells. 2013;31:1775-84 pubmed publisher
western blot; human	Ren G, Baritaki S, Marathe H, Feng J, Park S, Beach S, et al. Polycomb protein EZH2 regulates tumor invasion via the transcriptional repression of the metastasis suppressor RKIP in breast and prostate cancer. Cancer Res. 2012;72:3091-104 pubmed publisher
	Sehnalová P, Legartova S, Cmarko D, Kozubek S, Bartova E. Recruitment of HP1? to UVA-induced DNA lesions is independent of radiation-induced changes in A-type lamins. Biol Cell. 2014;106:151-65 pubmed publisher
	Guo S, Xu X, Tang Y, Zhang C, Li J, Ouyang Y, et al. miR-15a inhibits cell proliferation and epithelial to mesenchymal transition in pancreatic ductal adenocarcinoma by down-regulating Bmi-1 expression. Cancer Lett. 2014;344:40-46 pubmed publisher
	Sato A, Okada M, Shibuya K, Watanabe E, Seino S, Narita Y, et al. Pivotal role for ROS activation of p38 MAPK in the control of differentiation and tumor-initiating capacity of glioma-initiating cells. Stem Cell Res. 2014;12:119-31 pubmed publisher
	Liang W, Zhu D, Cui X, Su J, Liu H, Han J, et al. Knockdown BMI1 expression inhibits proliferation and invasion in human bladder cancer T24 cells. Mol Cell Biochem. 2013;382:283-91 pubmed publisher
	Sato A, Okada M, Shibuya K, Watanabe E, Seino S, Suzuki K, et al. Resveratrol promotes proteasome-dependent degradation of Nanog via p53 activation and induces differentiation of glioma stem cells. Stem Cell Res. 2013;11:601-10 pubmed publisher
	Harada Y, Inoue D, Ding Y, Imagawa J, Doki N, Matsui H, et al. RUNX1/AML1 mutant collaborates with BMI1 overexpression in the development of human and murine myelodysplastic syndromes. Blood. 2013;121:3434-46 pubmed publisher
	van Arensbergen J, GarcÃa Hurtado J, Maestro M, Correa Tapia M, Rutter G, Vidal M, et al. Ring1b bookmarks genes in pancreatic embryonic progenitors for repression in adult Î² cells. Genes Dev. 2013;27:52-63 pubmed publisher
	Erovic B, Kim D, Cassol C, Goldstein D, Irish J, Asa S, et al. Prognostic and predictive markers in medullary thyroid carcinoma. Endocr Pathol. 2012;23:232-42 pubmed publisher
	Legartova S, Jugova A, Stixova L, Kozubek S, Fojtova M, Zdrahal Z, et al. Epigenetic aspects of HP1 exchange kinetics in apoptotic chromatin. Biochimie. 2013;95:167-79 pubmed publisher
	Erovic B, Harris L, Jamali M, Goldstein D, Irish J, Asa S, et al. Biomarkers of parathyroid carcinoma. Endocr Pathol. 2012;23:221-31 pubmed publisher
	Liu Z, Ren G, Shangguan C, Guo L, Dong Z, Li Y, et al. ATRA inhibits the proliferation of DU145 prostate cancer cells through reducing the methylation level of HOXB13 gene. PLoS ONE. 2012;7:e40943 pubmed publisher
	Erovic B, Al Habeeb A, Harris L, Goldstein D, Kim D, Ghazarian D, et al. Identification of novel target proteins in sebaceous gland carcinoma. Head Neck. 2013;35:642-8 pubmed publisher
	Gu T, Liu S, Zheng P. Cytoplasmic NANOG-positive stromal cells promote human cervical cancer progression. Am J Pathol. 2012;181:652-61 pubmed publisher
	Nakagawa E, Zhang L, Shin J, Kim E, Cho S, Ohshima H, et al. The novel expression of Oct3/4 and Bmi1 in the root development of mouse molars. Cell Tissue Res. 2012;347:479-84 pubmed publisher
	Yu T, Chen X, Zhang W, Colon D, Shi J, Napier D, et al. Regulation of the potential marker for intestinal cells, Bmi1, by ?-catenin and the zinc finger protein KLF4: implications for colon cancer. J Biol Chem. 2012;287:3760-8 pubmed publisher
	Mao L, Ding J, Perdue A, Yang L, Zha Y, Ren M, et al. Cyclin E1 is a common target of BMI1 and MYCN and a prognostic marker for neuroblastoma progression. Oncogene. 2012;31:3785-95 pubmed publisher
	Sustáčková G, Kozubek S, Stixova L, Legartova S, Matula P, Orlova D, et al. Acetylation-dependent nuclear arrangement and recruitment of BMI1 protein to UV-damaged chromatin. J Cell Physiol. 2012;227:1838-50 pubmed publisher
	Wang Z, Park H, Carr J, Chen Y, Zheng Y, Li J, et al. FoxM1 in tumorigenicity of the neuroblastoma cells and renewal of the neural progenitors. Cancer Res. 2011;71:4292-302 pubmed publisher
	Pinho A, Rooman I, Real F. p53-dependent regulation of growth, epithelial-mesenchymal transition and stemness in normal pancreatic epithelial cells. Cell Cycle. 2011;10:1312-21 pubmed
	Sunayama J, Matsuda K, Sato A, Tachibana K, Suzuki K, Narita Y, et al. Crosstalk between the PI3K/mTOR and MEK/ERK pathways involved in the maintenance of self-renewal and tumorigenicity of glioblastoma stem-like cells. Stem Cells. 2010;28:1930-9 pubmed publisher
	Wang L, Jin Q, Lee J, Su I, Ge K. Histone H3K27 methyltransferase Ezh2 represses Wnt genes to facilitate adipogenesis. Proc Natl Acad Sci U S A. 2010;107:7317-22 pubmed publisher
	Kwiatkowski D, Thompson H, Bloom D. The polycomb group protein Bmi1 binds to the herpes simplex virus 1 latent genome and maintains repressive histone marks during latency. J Virol. 2009;83:8173-81 pubmed publisher

product information

Catalog Number :

05-637

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Bmi-1 Antibody, clone F6

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P35226;P25916

Host Name :

Mouse

Antigen :

Bmi-1

Clone :

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Bmi-1 Antibody, clone F6

Cross Reactivity :

Human;Mouse;Rat;Rabbit

Background :

The Bmi1 protein is a member of the polycomb group (PcG) proteins. PcG proteins form multiprotein complexes that function as transcriptional repressors. Several lines of evidence implicate Bmi1 in tumorigenesis. Firstly, Bmi1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. Secondly, Bmi1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene leads to neoplastic transformation. Thirdly, the BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. Fourthly, the transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires Bmi1. Indeed, based on a list of genes differentially expressed in a mouse model of metastatic prostate cancer on a wild-type and bmi1-deficient genetic background a gene signature was recently identified that may predict response to therapy and survival in multiple types of cancer.

ALT Names :

Polycomb group RING finger protein 4; RING finger protein 51

Immunogen :

Immunized with recombinant Bmi-1 protein corresponding to residues 1-202 of mouse Bmi-1.

Specificity :

Recognizes Bmi-1 (triplet).

Package Size :

100 μg

Uses :

Immunocytochemistry;Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at 2-8°C from date of receipt