phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor® 647 | EMD Millipore 05-636-AF647 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor® 647

catalog :

05-636-AF647

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

AF647

antigen modification :

phosphorylated

clone name :

JBW301

reactivity :

human, mouse

application :

immunohistochemistry, immunocytochemistry

citations: 5

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse	Davies B, Hatton E, Altemose N, Hussin J, Pratto F, Zhang G, et al. Re-engineering the zinc fingers of PRDM9 reverses hybrid sterility in mice. Nature. 2016;530:171-176 pubmed publisher
immunocytochemistry; mouse; fig 1	Cohen I, Idan C, Rider P, Peleg R, Vornov E, Elena V, et al. IL-1Î± is a DNA damage sensor linking genotoxic stress signaling to sterile inflammation and innate immunity. Sci Rep. 2015;5:14756 pubmed publisher
	Abe H, Alavattam K, Hu Y, Pang Q, Andreassen P, Hegde R, et al. The Initiation of Meiotic Sex Chromosome Inactivation Sequesters DNA Damage Signaling from Autosomes in Mouse Spermatogenesis. Curr Biol. 2019;: pubmed publisher
	Aubrey B, Janic A, Chen Y, Chang C, Lieschke E, Diepstraten S, et al. Mutant TRP53 exerts a target gene-selective dominant-negative effect to drive tumor development. Genes Dev. 2018;32:1420-1429 pubmed publisher
	Mancini A, Xavier Magalhães A, Woods W, Nguyen K, Amen A, Hayes J, et al. Disruption of the β1L Isoform of GABP Reverses Glioblastoma Replicative Immortality in a TERT Promoter Mutation-Dependent Manner. Cancer Cell. 2018;34:513-528.e8 pubmed publisher

product information

Catalog Number :

05-636-AF647

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor® 647

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P16104

Host Name :

Mouse

Antigen :

H2AFX

Clone :

JBW301

Conjugate :

AlexaFluor®647

Purification Method :

Protein G purified

Isotype :

IgG1

Product Description :

Anti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor® 647

Cross Reactivity :

Human

Background :

As a member of the histone H2A family, histone H2A.x (H2A.x) is a variant histone H2A which replaces conventional H2A in a subset of nucleosomes. H2A.x is involved in the DNA repair of double-strand breakage (DSB) damage on nuclear DNA. After a double strand DNA break, H2A.x is rapidly phosphorylated at serine 139 by ATM kinase and becomes gamma-H2AFX. This phosphorylation step can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. As a part of posttranslational modifications during apoptosis caused by severe DNA damage, high expression of phosphorylated H2A.x is considered as an accurate indicator of apoptosis.

ALT Names :

Histone H2AX, H2a/x, Histone H2A.X

Immunogen :

Linear peptide corresponding to phospho Histone H2A.X (Ser139).

Package Size :

100 µL

Uses :

Immunocytochemistry

Storage :

Stable for 1 year at 2-8°C from date of receipt