This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
RNA polymerase II Antibody, clone CTD4H8, Ascites Free
catalog :
05-623-Z
quantity :
200 μg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
CTD4H8

The same clone is also sold as:
reactivity :
Saccharomycetales, human, mouse, rat
application :
western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq
citations: 59
Published Application/Species/Sample/DilutionReference
  • immunoprecipitation; human; loading ...; fig 6d
Fujimoto M, Takii R, Katiyar A, Srivastava P, Nakai A. Poly(ADP-Ribose) Polymerase 1 Promotes the Human Heat Shock Response by Facilitating Heat Shock Transcription Factor 1 Binding to DNA. Mol Cell Biol. 2018;38: pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig 4c
Audet Walsh E, Yee T, Tam I, Giguere V. Inverse Regulation of DHT Synthesis Enzymes 5?-Reductase Types 1 and 2 by the Androgen Receptor in Prostate Cancer. Endocrinology. 2017;158:1015-1021 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig 4b
Hu T, Zhu X, Pi W, Yu M, Shi H, Tuan D. Hypermethylated LTR retrotransposon exhibits enhancer activity. Epigenetics. 2017;12:226-237 pubmed publisher
  • chromatin immunoprecipitation; mouse; loading ...; fig 5a
  • chromatin immunoprecipitation; human; loading ...; fig 5b
Vispute S, Bu P, Le Y, Cheng X. Activation of GR but not PXR by dexamethasone attenuated acetaminophen hepatotoxicities via Fgf21 induction. Toxicology. 2017;378:95-106 pubmed publisher
  • ChIP-Seq; human; fig 4
Gilan O, Lam E, Becher I, Lugo D, Cannizzaro E, Joberty G, et al. Functional interdependence of BRD4 and DOT1L in MLL leukemia. Nat Struct Mol Biol. 2016;23:673-81 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig 7e
Yamashita D, Moriuchi T, Osumi T, Hirose F. Transcription Factor hDREF Is a Novel SUMO E3 Ligase of Mi2α. J Biol Chem. 2016;291:11619-34 pubmed publisher
  • immunocytochemistry; human; 1:200; fig 6g
Shao Z, Zhang R, Khodadadi Jamayran A, Chen B, Crowley M, Festok M, et al. The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. Nat Commun. 2016;7:10869 pubmed publisher
  • chromatin immunoprecipitation; human; 1:100; fig 5b
Wang X, Jung Y, Jun S, Lee S, Wang W, Schneider A, et al. PAF-Wnt signaling-induced cell plasticity is required for maintenance of breast cancer cell stemness. Nat Commun. 2016;7:10633 pubmed publisher
  • chromatin immunoprecipitation; human
Madsen A, Bozickovic O, Bjune J, Mellgren G, Sagen J. Metformin inhibits hepatocellular glucose, lipid and cholesterol biosynthetic pathways by transcriptionally suppressing steroid receptor coactivator 2 (SRC-2). Sci Rep. 2015;5:16430 pubmed publisher
  • chromatin immunoprecipitation; mouse; fig 6
Tan K, Fujimoto M, Takii R, Takaki E, Hayashida N, Nakai A. Mitochondrial SSBP1 protects cells from proteotoxic stresses by potentiating stress-induced HSF1 transcriptional activity. Nat Commun. 2015;6:6580 pubmed publisher
  • western blot; human; fig 4
García Carmona Y, Cols M, Ting A, Radigan L, Yuk F, Zhang L, et al. Differential induction of plasma cells by isoforms of human TACI. Blood. 2015;125:1749-58 pubmed publisher
  • western blot; Saccharomycetales
Allepuz Fuster P, Martínez Fernández V, Garrido Godino A, Alonso Aguado S, Hanes S, Navarro F, et al. Rpb4/7 facilitates RNA polymerase II CTD dephosphorylation. Nucleic Acids Res. 2014;42:13674-88 pubmed
  • chromatin immunoprecipitation; human; fig 1
Maruyama A, Mimura J, Itoh K. Non-coding RNA derived from the region adjacent to the human HO-1 E2 enhancer selectively regulates HO-1 gene induction by modulating Pol II binding. Nucleic Acids Res. 2014;42:13599-614 pubmed publisher
  • chromatin immunoprecipitation; human
Wright R, Slemmons K, Vaughan A. Estradiol induces gene proximity and MLL-MLLT3 fusion in an activation-induced cytidine deaminase-mediated pathway. Leuk Lymphoma. 2015;56:1460-5 pubmed publisher
  • immunoprecipitation; Saccharomycetales
Mirón García M, Garrido Godino A, Martínez Fernández V, Fernández Pévida A, Cuevas Bermúdez A, Martín Expósito M, et al. The yeast prefoldin-like URI-orthologue Bud27 associates with the RSC nucleosome remodeler and modulates transcription. Nucleic Acids Res. 2014;42:9666-76 pubmed publisher
  • chromatin immunoprecipitation; human
Maruyama A, Mimura J, Harada N, Itoh K. Nrf2 activation is associated with Z-DNA formation in the human HO-1 promoter. Nucleic Acids Res. 2013;41:5223-34 pubmed publisher
Chappidi N, Nascakova Z, Boleslavska B, Zellweger R, Isik E, Andrs M, et al. Fork Cleavage-Religation Cycle and Active Transcription Mediate Replication Restart after Fork Stalling at Co-transcriptional R-Loops. Mol Cell. 2019;: pubmed publisher
Baquero Perez B, Antanaviciute A, Yonchev I, Carr I, Wilson S, Whitehouse A. The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus. elife. 2019;8: pubmed publisher
Rossi M, Bucci G, Rizzotto D, Bordo D, Marzi M, Puppo M, et al. LncRNA EPR controls epithelial proliferation by coordinating Cdkn1a transcription and mRNA decay response to TGF-β. Nat Commun. 2019;10:1969 pubmed publisher
Sharma P, Lioutas A, Fernandez Fuentes N, Quilez J, Carbonell Caballero J, Wright R, et al. Arginine Citrullination at the C-Terminal Domain Controls RNA Polymerase II Transcription. Mol Cell. 2019;73:84-96.e7 pubmed publisher
Bornelöv S, Reynolds N, Xenophontos M, Gharbi S, Johnstone E, Floyd R, et al. The Nucleosome Remodeling and Deacetylation Complex Modulates Chromatin Structure at Sites of Active Transcription to Fine-Tune Gene Expression. Mol Cell. 2018;71:56-72.e4 pubmed publisher
Cannizzaro E, Bannister A, Han N, Alendar A, Kouzarides T. DDX3X RNA helicase affects breast cancer cell cycle progression by regulating expression of KLF4. FEBS Lett. 2018;592:2308-2322 pubmed publisher
Kim M, Xia B, Suh H, Lee S, Jun S, Lien E, et al. PAF-Myc-Controlled Cell Stemness Is Required for Intestinal Regeneration and Tumorigenesis. Dev Cell. 2018;44:582-596.e4 pubmed publisher
Tan X, Banerjee P, Liu X, Yu J, Gibbons D, Wu P, et al. The epithelial-to-mesenchymal transition activator ZEB1 initiates a prometastatic competing endogenous RNA network. J Clin Invest. 2018;128:1267-1282 pubmed publisher
Chen J, Teo B, Cai Y, Wee S, Lu J. The linker histone H1.2 is a novel component of the nucleolar organizer regions. J Biol Chem. 2018;293:2358-2369 pubmed publisher
Bugge M, Bergstrom B, Eide O, Solli H, Kjønstad I, Stenvik J, et al. Surface Toll-like receptor 3 expression in metastatic intestinal epithelial cells induces inflammatory cytokine production and promotes invasiveness. J Biol Chem. 2017;292:15408-15425 pubmed publisher
Lu W, Katzenellenbogen B. Estrogen Receptor-? Modulation of the ER?-p53 Loop Regulating Gene Expression, Proliferation, and Apoptosis in Breast Cancer. Horm Cancer. 2017;8:230-242 pubmed publisher
Ono R, Masuya M, Ishii S, Katayama N, Nosaka T. Eya2, a Target Activated by Plzf, Is Critical for PLZF-RARA-Induced Leukemogenesis. Mol Cell Biol. 2017;37: pubmed publisher
Slobodin B, Han R, Calderone V, Vrielink J, Loayza Puch F, Elkon R, et al. Transcription Impacts the Efficiency of mRNA Translation via Co-transcriptional N6-adenosine Methylation. Cell. 2017;169:326-337.e12 pubmed publisher
Ma J, Sanchez B, Hall D, Tremblay A, Di Marco S, Gallouzi I. STAT3 promotes IFNγ/TNFα-induced muscle wasting in an NF-κB-dependent and IL-6-independent manner. EMBO Mol Med. 2017;9:622-637 pubmed publisher
Martínez Alonso M, Hengrung N, Fodor E. RNA-Free and Ribonucleoprotein-Associated Influenza Virus Polymerases Directly Bind the Serine-5-Phosphorylated Carboxyl-Terminal Domain of Host RNA Polymerase II. J Virol. 2016;90:6014-6021 pubmed publisher
Hsu C, Liu J, Wu P, Guan H, Chen Y, Lin A, et al. Identification of a new androgen receptor (AR) co-regulator BUD31 and related peptides to suppress wild-type and mutated AR-mediated prostate cancer growth via peptide screening and X-ray structure analysis. Mol Oncol. 2014;8:1575-87 pubmed publisher
Khurana J, Wang X, Chen X, Perlman D, Landweber L. Transcription-independent functions of an RNA polymerase II subunit, Rpb2, during genome rearrangement in the ciliate, Oxytricha trifallax. Genetics. 2014;197:839-49 pubmed publisher
Sonzogni S, Ogara M, Belluscio L, Castillo D, Scassa M, Cánepa E. p19INK4d is involved in the cellular senescence mechanism contributing to heterochromatin formation. Biochim Biophys Acta. 2014;1840:2171-83 pubmed publisher
Angulo Rojo C, Manning Cela R, Aguirre A, Ortega A, Lopez Bayghen E. Involvement of the Notch pathway in terminal astrocytic differentiation: role of PKA. ASN Neuro. 2013;5:e00130 pubmed publisher
Tanaka Nakanishi A, Yasunaga J, Takai K, Matsuoka M. HTLV-1 bZIP factor suppresses apoptosis by attenuating the function of FoxO3a and altering its localization. Cancer Res. 2014;74:188-200 pubmed publisher
Lim H, Dimova N, Tan M, Sigoillot F, King R, Shi Y. The G2/M regulator histone demethylase PHF8 is targeted for degradation by the anaphase-promoting complex containing CDC20. Mol Cell Biol. 2013;33:4166-80 pubmed publisher
Garrido Godino A, García López M, Navarro F. Correct assembly of RNA polymerase II depends on the foot domain and is required for multiple steps of transcription in Saccharomyces cerevisiae. Mol Cell Biol. 2013;33:3611-26 pubmed publisher
Chen W, Jia W, Wang K, Si X, Zhu S, Duan T, et al. Distinct roles for CBP and p300 on the RA-mediated expression of the meiosis commitment gene Stra8 in mouse embryonic stem cells. PLoS ONE. 2013;8:e66076 pubmed publisher
Udofa E, Stringer B, Gade P, Mahony D, Buzza M, Kalvakolanu D, et al. The transcription factor C/EBP-? mediates constitutive and LPS-inducible transcription of murine SerpinB2. PLoS ONE. 2013;8:e57855 pubmed publisher
Wong J, Mehta V, Voronova A, Coutu J, Ryan T, Shelton M, et al. ?-catenin is essential for efficient in vitro premyogenic mesoderm formation but can be partially compensated by retinoic acid signalling. PLoS ONE. 2013;8:e57501 pubmed publisher
Tashiro S, Asano T, Kanoh J, Ishikawa F. Transcription-induced chromatin association of RNA surveillance factors mediates facultative heterochromatin formation in fission yeast. Genes Cells. 2013;18:327-39 pubmed publisher
He S, Khan D, Winter S, Seiser C, Davie J. Dynamic distribution of HDAC1 and HDAC2 during mitosis: association with F-actin. J Cell Physiol. 2013;228:1525-35 pubmed publisher
Wier E, Neighoff J, Sun X, Fu K, Wan F. Identification of an N-terminal truncation of the NF-?B p65 subunit that specifically modulates ribosomal protein S3-dependent NF-?B gene expression. J Biol Chem. 2012;287:43019-29 pubmed publisher
Cherian M, Wilson E, Shapiro D. A competitive inhibitor that reduces recruitment of androgen receptor to androgen-responsive genes. J Biol Chem. 2012;287:23368-80 pubmed publisher
Jani D, Lutz S, Hurt E, Laskey R, Stewart M, Wickramasinghe V. Functional and structural characterization of the mammalian TREX-2 complex that links transcription with nuclear messenger RNA export. Nucleic Acids Res. 2012;40:4562-73 pubmed publisher
Ianculescu I, Wu D, Siegmund K, Stallcup M. Selective roles for cAMP response element-binding protein binding protein and p300 protein as coregulators for androgen-regulated gene expression in advanced prostate cancer cells. J Biol Chem. 2012;287:4000-13 pubmed publisher
Lee Y, Lee J, Ho C, Hong Q, Yu S, Tzeng C, et al. miRNA-34b as a tumor suppressor in estrogen-dependent growth of breast cancer cells. Breast Cancer Res. 2011;13:R116 pubmed publisher
Grbic D, Degagné E, Larrivée J, Bilodeau M, Vinette V, Arguin G, et al. P2Y6 receptor contributes to neutrophil recruitment to inflamed intestinal mucosa by increasing CXC chemokine ligand 8 expression in an AP-1-dependent manner in epithelial cells. Inflamm Bowel Dis. 2012;18:1456-69 pubmed publisher
Purcell D, Jeong K, Bittencourt D, Gerke D, Stallcup M. A distinct mechanism for coactivator versus corepressor function by histone methyltransferase G9a in transcriptional regulation. J Biol Chem. 2011;286:41963-71 pubmed publisher
Garcia Lopez M, Pelechano V, Mirón García M, Garrido Godino A, Garcia A, Calvo O, et al. The conserved foot domain of RNA pol II associates with proteins involved in transcriptional initiation and/or early elongation. Genetics. 2011;189:1235-48 pubmed publisher
Gorski J, Savage K, Mulligan J, McDade S, Blayney J, Ge Z, et al. Profiling of the BRCA1 transcriptome through microarray and ChIP-chip analysis. Nucleic Acids Res. 2011;39:9536-48 pubmed publisher
Furumai R, Ito A, Ogawa K, Maeda S, Saito A, Nishino N, et al. Histone deacetylase inhibitors block nuclear factor-?B-dependent transcription by interfering with RNA polymerase II recruitment. Cancer Sci. 2011;102:1081-7 pubmed publisher
Garcia A, Rosonina E, Manley J, Calvo O. Sub1 globally regulates RNA polymerase II C-terminal domain phosphorylation. Mol Cell Biol. 2010;30:5180-93 pubmed publisher
Pfenning A, Kim T, Spotts J, Hemberg M, Su D, West A. Genome-wide identification of calcium-response factor (CaRF) binding sites predicts a role in regulation of neuronal signaling pathways. PLoS ONE. 2010;5:e10870 pubmed publisher
Bitler B, Goverdhan A, Schroeder J. MUC1 regulates nuclear localization and function of the epidermal growth factor receptor. J Cell Sci. 2010;123:1716-23 pubmed publisher
Harte M, O Brien G, Ryan N, Gorski J, Savage K, Crawford N, et al. BRD7, a subunit of SWI/SNF complexes, binds directly to BRCA1 and regulates BRCA1-dependent transcription. Cancer Res. 2010;70:2538-47 pubmed publisher
Torres Padilla M, Zernicka Goetz M. Role of TIF1alpha as a modulator of embryonic transcription in the mouse zygote. J Cell Biol. 2006;174:329-38 pubmed
Shopland L, Lynch C, Peterson K, Thornton K, Kepper N, Hase J, et al. Folding and organization of a contiguous chromosome region according to the gene distribution pattern in primary genomic sequence. J Cell Biol. 2006;174:27-38 pubmed
product information
Catalog Number :
05-623-Z
Subcategory :
Epigenetics & Nuclear Function
Product Name :
Anti-RNA polymerase II Antibody, clone CTD4H8, Ascites Free
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P24928
Host Name :
Mouse
Antigen :
RNA polymerase II
Clone :
CTD4H8
Conjugate :
Purified
Isotype :
IgG1κ
Product Description :
Anti-RNA polymerase II Antibody, clone CTD4H8, Ascites Free
Cross Reactivity :
Human;Mouse;Rat;Yeast
Background :
RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
ALT Names :
DNA-directed RNA polymerase II subunit RPB1;RNA polymerase II subunit B1;DNA-directed RNA polymerase II subunit A;DNA-directed RNA polymerase III largest subunit;RNA-directed RNA polymerase II subunit RPB1
Immunogen :
Linear peptide corresponding to human RNA polymerase II.
Package Size :
200 μg
Uses :
Western Blotting;Immunocytochemistry;ChIP-seq
Storage :
Stable for 1 year at 2-8°C from date of receipt
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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