CUGBP1 Antibody, clone 3B1 | EMD Millipore 05-621 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

CUGBP1 Antibody, clone 3B1

catalog :

05-621

quantity :

200 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3B1

The same clone is also sold as:

LifeSpan Biosciences: LS-B216
Novus Biologicals: NB200-316, NBP2-80684
Abnova: MAB2351
OriGene: TA301501
Santa Cruz Biotechnology: sc-20003
Invitrogen: MA1-16675
Abcam: ab9549

reactivity :

human, mouse, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, EMSA, RNA immunoprecipitation

citations: 7

Published Application/Species/Sample/Dilution	Reference
RNA immunoprecipitation; human; 1:1000; loading ...; fig 4f flow cytometry; human; 1:1000; loading ...; fig st3 immunohistochemistry; human; 1:1000; loading ...; fig 6a western blot; human; 1:1000; loading ...; fig 4b	Chaudhury A, Cheema S, Fachini J, Kongchan N, Lu G, Simon L, et al. CELF1 is a central node in post-transcriptional regulatory programmes underlying EMT. Nat Commun. 2016;7:13362 pubmed publisher
western blot; mouse; fig 2	Kim Y, Yadava R, Mandal M, Mahadevan K, Yu Q, Leitges M, et al. Disease Phenotypes in a Mouse Model of RNA Toxicity Are Independent of Protein Kinase Cα and Protein Kinase Cβ. PLoS ONE. 2016;11:e0163325 pubmed publisher
western blot; human; 1:2000; fig 1	Laustriat D, Gide J, Barrault L, Chautard E, Benoit C, Auboeuf D, et al. In Vitro and In Vivo Modulation of Alternative Splicing by the Biguanide Metformin. Mol Ther Nucleic Acids. 2015;4:e262 pubmed publisher
immunoprecipitation; human western blot; human	Jayaseelan S, Doyle F, Tenenbaum S. Profiling post-transcriptionally networked mRNA subsets using RIP-Chip and RIP-Seq. Methods. 2014;67:13-9 pubmed publisher
	Giudice J, Xia Z, Li W, Cooper T. Neonatal cardiac dysfunction and transcriptome changes caused by the absence of Celf1. Sci Rep. 2016;6:35550 pubmed publisher
	Giudice J, Xia Z, Wang E, Scavuzzo M, Ward A, Kalsotra A, et al. Alternative splicing regulates vesicular trafficking genes in cardiomyocytes during postnatal heart development. Nat Commun. 2014;5:3603 pubmed publisher
	O Leary D, Vargas L, Sharif O, Garcia M, Sigal Y, Chow S, et al. HTS-Compatible Patient-Derived Cell-Based Assay to Identify Small Molecule Modulators of Aberrant Splicing in Myotonic Dystrophy Type 1. Curr Chem Genomics. 2010;4:9-18 pubmed publisher

product information

Catalog Number :

05-621

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-CUGBP1 Antibody, clone 3B1

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q92879

Host Name :

Mouse

Antigen :

CUGBP1

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-CUGBP1 Antibody, clone 3B1

Cross Reactivity :

Bovine;Human;Mouse;Pig;Rat;Rabbit

Background :

Myotonic dystrophy (MD) is an autosomal dominant neuromuscular disease that is associated with a (CTG)n repeat expansion in the 3'-untranslated region of the myotonin protein kinase (Mt-PK) gene. A (CUG)n oligonucleotides triplet repeat pre-mRNA/mRNA binding protein may play an important role in DM pathogenesis. HeLa cell protein, CUG-BP1, has been purified based upon its ability to bind specifically to (CUG)8 oligonucleotides in vitro. CUG-BP1 is the major (CUG)8 binding activity in normal cells. CUG-BP1 has been identified as isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50. The CUG-BP/hNab50 protein is localized predominantly in the nucleus and is associated with polyadenylated RNAs in vivo. In vitro RNA-binding/photocrosslinking studies demonstrate that CUG-BP/hNab50 binds to RNAs containing the Mt-PK 3'-UTR.

Immunogen :

Full-length GST fusion protein corresponding to human CUG-BP1, also known as heterogeneous nuclear ribonucleoprotein (hnRNP) hNab50

Specificity :

CUG-BP1

Package Size :

200 µg

Uses :

Electrophoretic Mobility Shift Assay;Immunoprecipitation;Western Blotting;Immunocytochemistry

Storage :

2 years at -20°C