LEF Antibody, clone 2D12 | EMD Millipore 05-602 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

LEF Antibody, clone 2D12

catalog :

05-602

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2D12

The same clone is also sold as:

OriGene: AM00083PU-N
Santa Cruz Biotechnology: sc-81470

reactivity :

human, mouse

application :

western blot

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 4b	Domingues M, Martinez Sanz J, Papon L, Larue L, Mouawad L, Bonaventure J. Structure-based mutational analysis of ICAT residues mediating negative regulation of ?-catenin co-transcriptional activity. PLoS ONE. 2017;12:e0172603 pubmed publisher
	Weise A, Bruser K, Elfert S, Wallmen B, Wittel Y, Wöhrle S, et al. Alternative splicing of Tcf7l2 transcripts generates protein variants with differential promoter-binding and transcriptional activation properties at Wnt/beta-catenin targets. Nucleic Acids Res. 2010;38:1964-81 pubmed publisher

product information

Catalog Number :

05-602

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-LEF Antibody, clone 2D12

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q9UJU2

Host Name :

Mouse

Antigen :

LEF

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-LEF Antibody, clone 2D12

Cross Reactivity :

Human;Mouse

Background :

Monoclonal Anti-LEF-1 (β-Catenin Binding Domain) recognizes the β-Catenin Binding Domain of human LEF-1 using immunoblotting (55-57 kDa). Lymphoid enhancer factor, LEF-1, is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play crucial roles in WNT/Wingless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer. LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve.

Immunogen :

Residues 1-85 of human Lymphoid Enhancer Factor (LEF)

Specificity :

LEF that appears as multiple polypeptides

Package Size :

100 µg

Uses :

Western Blotting

Storage :

2 years at -20°C