Akt/PKB Antibody, PH Domain, clone SKB1 | EMD Millipore 05-591MG product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Akt/PKB Antibody, PH Domain, clone SKB1

catalog :

05-591MG

quantity :

1 mg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SKB1

reactivity :

human, mouse

application :

western blot, immunoprecipitation

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:500; loading ...; fig 7a	Castella B, Kopecka J, Sciancalepore P, Mandili G, Foglietta M, Mitro N, et al. The ATP-binding cassette transporter A1 regulates phosphoantigen release and Vγ9Vδ2 T cell activation by dendritic cells. Nat Commun. 2017;8:15663 pubmed publisher
	Zhang W, Xiao H, Parkin K. Apoptosis in MCF-7 breast cancer cells induced by S-alkenylmercaptocysteine (CySSR) species derived from Allium tissues in combination with sodium selenite. Food Chem Toxicol. 2014;68:1-10 pubmed publisher

product information

Catalog Number :

05-591MG

Subcategory :

Cell Signaling

Product Name :

Anti-Akt/PKB Antibody, PH Domain, clone SKB1

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P31749

Host Name :

Mouse

Antigen :

Akt/PKB

Clone :

Clone SKB1

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-Akt/PKB Antibody, PH Domain, clone SKB1

Cross Reactivity :

Human;Mouse;Rat

Background :

Akt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.

Immunogen :

GST-fusion protein corresponding to residues 1-149 of human Akt

Specificity :

Recognizes Akt1/PKBα Akt2/PKBβ (30%) Akt3/PKBγ (weak to none) based on immunoblot analysis using 100 ng recombinant fusion protein for each isoform.

Package Size :

1 mg

Uses :

Immunoprecipitation;Western Blotting

Storage :

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.