Akt/PKB Antibody, PH Domain, clone SKB1 | EMD Millipore 05-591 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Akt/PKB Antibody, PH Domain, clone SKB1

catalog :

05-591

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SKB1

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry, other

citations: 23

Published Application/Species/Sample/Dilution	Reference
other; human; 1:5000; loading ...; fig 2a, 3a, 4a, 5a	Smolko C, Janes K. An ultrasensitive fiveplex activity assay for cellular kinases. Sci Rep. 2019;9:19409 pubmed publisher
western blot; human; 1:500; loading ...; fig 7a	Castella B, Kopecka J, Sciancalepore P, Mandili G, Foglietta M, Mitro N, et al. The ATP-binding cassette transporter A1 regulates phosphoantigen release and Vγ9Vδ2 T cell activation by dendritic cells. Nat Commun. 2017;8:15663 pubmed publisher
western blot; human; 1:500; loading ...; fig s1b	Kuo W, Hwu L, Wu C, Lee J, Chang C, Liu R. STAT3/NF-?B-Regulated Lentiviral TK/GCV Suicide Gene Therapy for Cisplatin-Resistant Triple-Negative Breast Cancer. Theranostics. 2017;7:647-663 pubmed publisher
western blot; rat; loading ...; fig 7e	Houddane A, Bultot L, Novellasdemunt L, Johanns M, Gueuning M, Vertommen D, et al. Role of Akt/PKB and PFKFB isoenzymes in the control of glycolysis, cell proliferation and protein synthesis in mitogen-stimulated thymocytes. Cell Signal. 2017;34:23-37 pubmed publisher
western blot; human; 1:1000; loading ...	Merckx E, Albertini G, Paterka M, Jensen C, Albrecht P, Dietrich M, et al. Absence of system xc- on immune cells invading the central nervous system alleviates experimental autoimmune encephalitis. J Neuroinflammation. 2017;14:9 pubmed publisher
western blot; human; 1:1000; fig 1	Carbonneau M, M Gagné L, Lalonde M, Germain M, Motorina A, Guiot M, et al. The oncometabolite 2-hydroxyglutarate activates the mTOR signalling pathway. Nat Commun. 2016;7:12700 pubmed publisher
western blot; human	Bawadekar M, de Andrea M, Lo Cigno I, Baldanzi G, Caneparo V, Graziani A, et al. The Extracellular IFI16 Protein Propagates Inflammation in Endothelial Cells Via p38 MAPK and NF-ÎºB p65 Activation. J Interferon Cytokine Res. 2015;35:441-53 pubmed publisher
western blot; mouse	Young M, Brewer R, Peliciari Garcia R, Collins H, He L, Birky T, et al. Cardiomyocyte-specific BMAL1 plays critical roles in metabolism, signaling, and maintenance of contractile function of the heart. J Biol Rhythms. 2014;29:257-76 pubmed publisher
western blot; human	Glorieux C, Auquier J, Dejeans N, Sid B, Demoulin J, Bertrand L, et al. Catalase expression in MCF-7 breast cancer cells is mainly controlled by PI3K/Akt/mTor signaling pathway. Biochem Pharmacol. 2014;89:217-23 pubmed publisher
western blot; mouse; fig 5	Pulinilkunnil T, Kienesberger P, Nagendran J, Sharma N, Young M, Dyck J. Cardiac-specific adipose triglyceride lipase overexpression protects from cardiac steatosis and dilated cardiomyopathy following diet-induced obesity. Int J Obes (Lond). 2014;38:205-15 pubmed publisher
	Dong C, Rovnaghi C, Anand K. Ketamine affects the neurogenesis of rat fetal neural stem progenitor cells via the PI3K/Akt-p27 signaling pathway. Birth Defects Res B Dev Reprod Toxicol. 2014;101:355-63 pubmed publisher
	Zhang W, Xiao H, Parkin K. Apoptosis in MCF-7 breast cancer cells induced by S-alkenylmercaptocysteine (CySSR) species derived from Allium tissues in combination with sodium selenite. Food Chem Toxicol. 2014;68:1-10 pubmed publisher
	Yue M, Li Q, Zhang Y, Zhao Y, Zhang Z, Bao S. Histone H4R3 methylation catalyzed by SKB1/PRMT5 is required for maintaining shoot apical meristem. PLoS ONE. 2013;8:e83258 pubmed publisher
	Tsai S, Lu C, Lee C, Lin Y, Chung J, Kuo S, et al. AKT serine/threonine protein kinase modulates bufalin-triggered intrinsic pathway of apoptosis in CAL 27 human oral cancer cells. Int J Oncol. 2012;41:1683-92 pubmed publisher
	Lin H, Chen Y, Hsu W, Yang C, Kao C, Tsai T. Resveratrol helps recovery from fatty liver and protects against hepatocellular carcinoma induced by hepatitis B virus X protein in a mouse model. Cancer Prev Res (Phila). 2012;5:952-62 pubmed publisher
	Jones L, Antonelli J, Masko E, Broadwater G, Lascola C, Fels D, et al. Exercise modulation of the host-tumor interaction in an orthotopic model of murine prostate cancer. J Appl Physiol (1985). 2012;113:263-72 pubmed publisher
	Stains C, Tedford N, Walkup T, Luković E, Goguen B, Griffith L, et al. Interrogating signaling nodes involved in cellular transformations using kinase activity probes. Chem Biol. 2012;19:210-7 pubmed publisher
	Gannon J, Ohlendieck K. Subproteomic analysis of basic proteins in aged skeletal muscle following offgel pre-fractionation. Mol Med Rep. 2012;5:993-1000 pubmed publisher
	Pulinilkunnil T, He H, Kong D, Asakura K, Peroni O, Lee A, et al. Adrenergic regulation of AMP-activated protein kinase in brown adipose tissue in vivo. J Biol Chem. 2011;286:8798-809 pubmed publisher
	Lapchak P, SCHUBERT D, Maher P. Delayed treatment with a novel neurotrophic compound reduces behavioral deficits in rabbit ischemic stroke. J Neurochem. 2011;116:122-31 pubmed publisher
	Treebak J, Taylor E, Witczak C, An D, Toyoda T, Koh H, et al. Identification of a novel phosphorylation site on TBC1D4 regulated by AMP-activated protein kinase in skeletal muscle. Am J Physiol Cell Physiol. 2010;298:C377-85 pubmed publisher
	Gannon J, Doran P, Kirwan A, Ohlendieck K. Drastic increase of myosin light chain MLC-2 in senescent skeletal muscle indicates fast-to-slow fibre transition in sarcopenia of old age. Eur J Cell Biol. 2009;88:685-700 pubmed publisher
	Lin H, Chiou S, Wu C, Lin W, Chen L, Yang Y, et al. Combretastatin A4-induced differential cytotoxicity and reduced metastatic ability by inhibition of AKT function in human gastric cancer cells. J Pharmacol Exp Ther. 2007;323:365-73 pubmed

product information

Catalog Number :

05-591

Subcategory :

Cell Signaling

Product Name :

Anti-Akt/PKB Antibody, PH Domain, clone SKB1

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P31749

Host Name :

Mouse

Antigen :

Akt/PKB

Clone :

SKB1

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-Akt/PKB Antibody, PH Domain, clone SKB1

Cross Reactivity :

Human;Mouse;Rat

Background :

Akt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.

Immunogen :

GST-fusion protein corresponding to residues 1-149 of human Akt1

Specificity :

Recognizes Akt1/PKBα.

Package Size :

100 μg

Uses :

Flow Cytometry;Immunocytochemistry;Immunoprecipitation;Western Blotting

Storage :

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.