FAK Antibody, clone 4.47 | EMD Millipore 05-537 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

FAK Antibody, clone 4.47

catalog :

05-537

quantity :

200 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4.47

The same clone is also sold as:

Abnova: MAB12511

reactivity :

human, mouse, rat, Xenopus laevis

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, other, western blot knockout validation, immunohistochemistry knockout validation, immunocytochemistry knockout validation

citations: 61

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry knockout validation; mouse; fig 1c immunohistochemistry knockout validation; mouse; fig 2c western blot knockout validation; mouse; fig 1b immunohistochemistry - paraffin section; mouse; fig 2c	van Miltenburg M, Lalai R, de Bont H, van Waaij E, Beggs H, Danen E, et al. Complete focal adhesion kinase deficiency in the mammary gland causes ductal dilation and aberrant branching morphogenesis through defects in Rho kinase-dependent cell contractility. FASEB J. 2009;23:3482-93 pubmed publisher
western blot knockout validation; mouse; loading ...; fig 1c immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 1a western blot; human; loading ...; fig 2a	Megison M, Gillory L, Stewart J, Nabers H, Mrozcek Musulman E, Beierle E. FAK inhibition abrogates the malignant phenotype in aggressive pediatric renal tumors. Mol Cancer Res. 2014;12:514-26 pubmed publisher
western blot; human; 1:1000; loading ...; fig 4b, 6s1c western blot; mouse; 1:1000; loading ...; fig 2c	Diaz Osterman C, Ozmadenci D, Kleinschmidt E, Taylor K, Barrie A, Jiang S, et al. FAK activity sustains intrinsic and acquired ovarian cancer resistance to platinum chemotherapy. elife. 2019;8: pubmed publisher
immunohistochemistry - paraffin section; human; 1:500; fig 1a western blot; human; loading ...; fig 2a	Waters A, Stafman L, Garner E, Mruthyunjayappa S, Stewart J, Mroczek Musulman E, et al. Targeting Focal Adhesion Kinase Suppresses the Malignant Phenotype in Rhabdomyosarcoma Cells. Transl Oncol. 2016;9:263-73 pubmed publisher
western blot; human; loading ...; fig 2b	Barcus C, Keely P, Eliceiri K, Schuler L. Prolactin signaling through focal adhesion complexes is amplified by stiff extracellular matrices in breast cancer cells. Oncotarget. 2016;7:48093-48106 pubmed publisher
immunoprecipitation; human; fig 2 western blot; human; fig 2	Budina Kolomets A, Webster M, Leu J, Jennis M, Krepler C, Guerrini A, et al. HSP70 Inhibition Limits FAK-Dependent Invasion and Enhances the Response to Melanoma Treatment with BRAF Inhibitors. Cancer Res. 2016;76:2720-30 pubmed publisher
immunocytochemistry; human; 1:500; fig 8	Petridou N, Skourides P. A ligand-independent integrin Î²1 mechanosensory complex guides spindle orientation. Nat Commun. 2016;7:10899 pubmed publisher
western blot; human; loading ...	Waters A, Stewart J, Atigadda V, Mroczek Musulman E, Muccio D, Grubbs C, et al. Preclinical Evaluation of UAB30 in Pediatric Renal and Hepatic Malignancies. Mol Cancer Ther. 2016;15:911-21 pubmed publisher
immunocytochemistry; human; 1:50; fig 4 western blot; human; 1:800; fig 6	Swaminathan V, Fischer R, Waterman C. The FAK-Arp2/3 interaction promotes leading edge advance and haptosensing by coupling nascent adhesions to lamellipodia actin. Mol Biol Cell. 2016;27:1085-100 pubmed publisher
other; human; loading ...; fig st1	Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
western blot; human; fig 4	Dave J, Abbey C, Duran C, Seo H, Johnson G, Bayless K. Hic-5 mediates the initiation of endothelial sprouting by regulating a key surface metalloproteinase. J Cell Sci. 2016;129:743-56 pubmed publisher
western blot; human	Picot N, Guerrette R, Beauregard A, Jean S, Michaud P, Harquail J, et al. Mammaglobin 1 promotes breast cancer malignancy and confers sensitivity to anticancer drugs. Mol Carcinog. 2016;55:1150-62 pubmed publisher
immunoprecipitation; human western blot; human western blot; mouse	Zhang J, Gao Q, Zhou Y, Dier U, Hempel N, Hochwald S. Focal adhesion kinase-promoted tumor glucose metabolism is associated with a shift of mitochondrial respiration to glycolysis. Oncogene. 2016;35:1926-42 pubmed publisher
immunocytochemistry; human; fig 4	Trerotola M, Ganguly K, Fazli L, Fedele C, Lu H, Dutta A, et al. Trop-2 is up-regulated in invasive prostate cancer and displaces FAK from focal contacts. Oncotarget. 2015;6:14318-28 pubmed
western blot; mouse	Tancioni I, Miller N, Uryu S, Lawson C, Jean C, Chen X, et al. FAK activity protects nucleostemin in facilitating breast cancer spheroid and tumor growth. Breast Cancer Res. 2015;17:47 pubmed publisher
western blot; human; 1:1000	Gillory L, Stewart J, Megison M, Waters A, Beierle E. Focal adhesion kinase and p53 synergistically decrease neuroblastoma cell survival. J Surg Res. 2015;196:339-49 pubmed publisher
western blot; human; 1:1500; fig 1	Morris E, Assi K, Salh B, Dedhar S. Integrin-linked kinase links dynactin-1/dynactin-2 with cortical integrin receptors to orient the mitotic spindle relative to the substratum. Sci Rep. 2015;5:8389 pubmed publisher
western blot; human; 1:3000	Curran C, Carrillo E, Ponik S, Keely P. Collagen density regulates xenobiotic and hypoxic response of mammary epithelial cells. Environ Toxicol Pharmacol. 2015;39:114-24 pubmed publisher
immunohistochemistry - paraffin section; human	Golubovskaya V, Ylagan L, Miller A, Hughes M, Wilson J, Wang D, et al. High focal adhesion kinase expression in breast carcinoma is associated with lymphovascular invasion and triple-negative phenotype. BMC Cancer. 2014;14:769 pubmed publisher
western blot; Xenopus laevis; 1:1000	Bjerke M, Dzamba B, Wang C, DeSimone D. FAK is required for tension-dependent organization of collective cell movements in Xenopus mesendoderm. Dev Biol. 2014;394:340-56 pubmed publisher
immunohistochemistry; human	Dy G, Ylagan L, Pokharel S, Miller A, Brese E, Bshara W, et al. The prognostic significance of focal adhesion kinase expression in stage I non-small-cell lung cancer. J Thorac Oncol. 2014;9:1278-84 pubmed publisher
western blot; human	Son J, Jeong H, Kim H, Kim Y, Lee E, Lee H, et al. Pelargonidin attenuates PDGF-BB-induced aortic smooth muscle cell proliferation and migration by direct inhibition of focal adhesion kinase. Biochem Pharmacol. 2014;89:236-45 pubmed publisher
immunohistochemistry - paraffin section; human western blot; human	Yoon H, Choi Y, Song J, Do I, Kang S, Ko Y, et al. Targeted inhibition of FAK, PYK2 and BCL-XL synergistically enhances apoptosis in ovarian clear cell carcinoma cell lines. PLoS ONE. 2014;9:e88587 pubmed publisher
immunoprecipitation; human western blot; human	Thapa N, Choi S, Hedman A, Tan X, Anderson R. Phosphatidylinositol phosphate 5-kinase I?i2 in association with Src controls anchorage-independent growth of tumor cells. J Biol Chem. 2013;288:34707-18 pubmed publisher
immunoprecipitation; human western blot; human	Dave J, Kang H, Abbey C, Maxwell S, Bayless K. Proteomic profiling of endothelial invasion revealed receptor for activated C kinase 1 (RACK1) complexed with vimentin to regulate focal adhesion kinase (FAK). J Biol Chem. 2013;288:30720-33 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100 immunocytochemistry; human; 1:1000 western blot; human	Stewart J, Ma X, Megison M, Nabers H, Cance W, Kurenova E, et al. Inhibition of FAK and VEGFR-3 binding decreases tumorigenicity in neuroblastoma. Mol Carcinog. 2015;54:9-23 pubmed publisher
western blot; human; 1:1000	Henderson Y, Toro Serra R, Chen Y, Ryu J, Frederick M, Zhou G, et al. Src inhibitors in suppression of papillary thyroid carcinoma growth. Head Neck. 2014;36:375-84 pubmed publisher
western blot; human; fig s4	Barcus C, Keely P, Eliceiri K, Schuler L. Stiff collagen matrices increase tumorigenic prolactin signaling in breast cancer cells. J Biol Chem. 2013;288:12722-32 pubmed publisher
western blot; human immunohistochemistry - paraffin section; mouse; 1:200	Megison M, Stewart J, Nabers H, Gillory L, Beierle E. FAK inhibition decreases cell invasion, migration and metastasis in MYCN amplified neuroblastoma. Clin Exp Metastasis. 2013;30:555-68 pubmed publisher
immunocytochemistry; human; 1:1000 western blot; human; fig s10	Beierle E, Ma X, Stewart J, Megison M, Cance W, Kurenova E. Inhibition of the focal adhesion kinase and vascular endothelial growth factor receptor-3 interaction leads to decreased survival in human neuroblastoma cell lines. Mol Carcinog. 2014;53:230-42 pubmed publisher
immunocytochemistry; human	Vickerman V, Kamm R. Mechanism of a flow-gated angiogenesis switch: early signaling events at cell-matrix and cell-cell junctions. Integr Biol (Camb). 2012;4:863-74 pubmed publisher
	Kim Y, Kim H, Kim H, Gawk H, Bae S, Sim H, et al. FAK-Copy-Gain Is a Predictive Marker for Sensitivity to FAK Inhibition in Breast Cancer. Cancers (Basel). 2019;11: pubmed publisher
	Zhao W, Liu D, Wei J, Miao Z, Zhang K, Su Z, et al. Caspr1 is a host receptor for meningitis-causing Escherichia coli. Nat Commun. 2018;9:2296 pubmed publisher
	Benzina S, Harquail J, Guerrette R, O Brien P, Jean S, Crapoulet N, et al. Breast Cancer Malignant Processes are Regulated by Pax-5 Through the Disruption of FAK Signaling Pathways. J Cancer. 2016;7:2035-2044 pubmed publisher
	Kir D, Saluja M, Modi S, Venkatachalam A, Schnettler E, Roy S, et al. Cell-permeable iron inhibits vascular endothelial growth factor receptor-2 signaling and tumor angiogenesis. Oncotarget. 2016;7:65348-65363 pubmed publisher
	Harizanova J, Fermin Y, Malik Sheriff R, Wieczorek J, Ickstadt K, Grecco H, et al. Highly Multiplexed Imaging Uncovers Changes in Compositional Noise within Assembling Focal Adhesions. PLoS ONE. 2016;11:e0160591 pubmed publisher
	Huynh N, Everts V, Leethanakul C, Pavasant P, Ampornaramveth R. Rinsing with Saline Promotes Human Gingival Fibroblast Wound Healing In Vitro. PLoS ONE. 2016;11:e0159843 pubmed publisher
	Gao C, Chen G, Kuan S, Zhang D, Schlaepfer D, Hu J. FAK/PYK2 promotes the Wnt/Î²-catenin pathway and intestinal tumorigenesis by phosphorylating GSK3Î². elife. 2015;4: pubmed publisher
	Williams K, Bundred N, Landberg G, Clarke R, Farnie G. Focal adhesion kinase and Wnt signaling regulate human ductal carcinoma in situ stem cell activity and response to radiotherapy. Stem Cells. 2015;33:327-41 pubmed publisher
	van Miltenburg M, van Nimwegen M, Tijdens I, Lalai R, Kuiper R, Klarenbeek S, et al. Mammary gland-specific ablation of focal adhesion kinase reduces the incidence of p53-mediated mammary tumour formation. Br J Cancer. 2014;110:2747-55 pubmed publisher
	Wan H, Mruk D, Wong C, Cheng C. Perfluorooctanesulfonate (PFOS) perturbs male rat Sertoli cell blood-testis barrier function by affecting F-actin organization via p-FAK-Tyr(407): an in vitro study. Endocrinology. 2014;155:249-62 pubmed publisher
	Chen Y, Lu H, Hsu S, Kuo C, Chang S, Lin J, et al. Bufalin inhibits migration and invasion in human hepatocellular carcinoma SK-Hep1 cells through the inhibitions of NF-kB and matrix metalloproteinase-2/-9-signaling pathways. Environ Toxicol. 2015;30:74-82 pubmed publisher
	Kawada I, Hasina R, Lennon F, Bindokas V, Usatyuk P, Tan Y, et al. Paxillin mutations affect focal adhesions and lead to altered mitochondrial dynamics: relevance to lung cancer. Cancer Biol Ther. 2013;14:679-91 pubmed publisher
	Lai W, Hsu S, Chueh F, Chen Y, Yang J, Lin J, et al. Quercetin inhibits migration and invasion of SAS human oral cancer cells through inhibition of NF-?B and matrix metalloproteinase-2/-9 signaling pathways. Anticancer Res. 2013;33:1941-50 pubmed
	Fromont G, Godet J, Peyret A, Irani J, Celhay O, Rozet F, et al. 8q24 amplification is associated with Myc expression and prostate cancer progression and is an independent predictor of recurrence after radical prostatectomy. Hum Pathol. 2013;44:1617-23 pubmed publisher
	Lu C, Yang J, Chiang J, Hour M, Amagaya S, Lu K, et al. Inhibition of invasion and migration by newly synthesized quinazolinone MJ-29 in human oral cancer CAL 27 cells through suppression of MMP-2/9 expression and combined down-regulation of MAPK and AKT signaling. Anticancer Res. 2012;32:2895-903 pubmed
	Bougault C, Aubert Foucher E, Paumier A, Perrier Groult E, Huot L, Hot D, et al. Dynamic compression of chondrocyte-agarose constructs reveals new candidate mechanosensitive genes. PLoS ONE. 2012;7:e36964 pubmed publisher
	Welman A, Sproul D, Mullen P, Muir M, Kinnaird A, Harrison D, et al. Diversity of matriptase expression level and function in breast cancer. PLoS ONE. 2012;7:e34182 pubmed publisher
	Lahlou H, Sanguin Gendreau V, Frame M, Muller W. Focal adhesion kinase contributes to proliferative potential of ErbB2 mammary tumour cells but is dispensable for ErbB2 mammary tumour induction in vivo. Breast Cancer Res. 2012;14:R36 pubmed
	Shaw C, Grobmyer S, Ucar D, Cance W, Reith J, Hochwald S. Elevated expression of IRS2 in the progression from neurofibroma to malignant peripheral nerve sheath tumor. Anticancer Res. 2012;32:439-43 pubmed
	Huang C, Fu X, Liu J, Qi Y, Li S, Wang H. The involvement of integrin ?1 signaling in the migration and myofibroblastic differentiation of skin fibroblasts on anisotropic collagen-containing nanofibers. Biomaterials. 2012;33:1791-800 pubmed publisher
	Wortmann A, He Y, Christensen M, Linn M, Lumley J, Pollock P, et al. Cellular settings mediating Src Substrate switching between focal adhesion kinase tyrosine 861 and CUB-domain-containing protein 1 (CDCP1) tyrosine 734. J Biol Chem. 2011;286:42303-15 pubmed publisher
	Makrodouli E, Oikonomou E, Koc M, Andera L, Sasazuki T, Shirasawa S, et al. BRAF and RAS oncogenes regulate Rho GTPase pathways to mediate migration and invasion properties in human colon cancer cells: a comparative study. Mol Cancer. 2011;10:118 pubmed publisher
	Eke I, Cordes N. Dual targeting of EGFR and focal adhesion kinase in 3D grown HNSCC cell cultures. Radiother Oncol. 2011;99:279-86 pubmed publisher
	Tall E, Bernstein A, Oliver N, Gray J, Masur S. TGF-?-stimulated CTGF production enhanced by collagen and associated with biogenesis of a novel 31-kDa CTGF form in human corneal fibroblasts. Invest Ophthalmol Vis Sci. 2010;51:5002-11 pubmed publisher
	Sun Y, Ling K, Wagoner M, Anderson R. Type I gamma phosphatidylinositol phosphate kinase is required for EGF-stimulated directional cell migration. J Cell Biol. 2007;178:297-308 pubmed
	Kornberg L, Grant M. Adenoviruses increase endothelial cell proliferation, migration, and tube formation: partial reversal by the focal adhesion kinase inhibitor, FRNK. Microvasc Res. 2007;73:157-62 pubmed
	Banu N, Teichman J, Dunlap Brown M, Villegas G, Tufro A. Semaphorin 3C regulates endothelial cell function by increasing integrin activity. FASEB J. 2006;20:2150-2 pubmed
	Guan F, Villegas G, Teichman J, Mundel P, Tufro A. Autocrine VEGF-A system in podocytes regulates podocin and its interaction with CD2AP. Am J Physiol Renal Physiol. 2006;291:F422-8 pubmed
	Hoffman L, Jensen C, Kloeker S, Wang C, Yoshigi M, Beckerle M. Genetic ablation of zyxin causes Mena/VASP mislocalization, increased motility, and deficits in actin remodeling. J Cell Biol. 2006;172:771-82 pubmed
	Ling K, Doughman R, Iyer V, Firestone A, Bairstow S, Mosher D, et al. Tyrosine phosphorylation of type Igamma phosphatidylinositol phosphate kinase by Src regulates an integrin-talin switch. J Cell Biol. 2003;163:1339-49 pubmed

product information

Catalog Number :

05-537

Subcategory :

Cell Signaling

Product Name :

Anti-FAK Antibody, clone 4.47

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q05397

Host Name :

Mouse

Antigen :

FAK

Clone :

4.47

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-FAK Antibody, clone 4.47

Cross Reactivity :

Human;Mouse;Rat

Background :

Focal adhesion kinase (FAK) is a cytoplasmic protein tyrosine kinase that has been implicated to play an important role in integrin-mediated signal transduction pathways. FAK becomes activated and tyrosine phosphorylated in integrin-mediated cell adhesion and is colocalized with integrins and other cytoskeletal proteins in focal contacts in a variety of adherent cells. Physical interactions of FAK with the integrin cytoplasmic domain as well as cytoskeletal proteins talin, paxillin, and/ or tensin have been proposed to play a key role in FAK activation by facilitating its oligomerization and transphosphorylation. FAK shows a rapid increase in tyrosine phosphorylation when stimulated by diverse signaling molecules, including those that regulate embryonic development, cell proliferation, migration and apoptosis, and efficient study methods are in high demand.

Immunogen :

GST fusion protein corresponding to residues 1-423 of human FAK (focal adhesion kinase); specific for p125FAK

Specificity :

Recognizes p125FAK; does not cross react with Pyk-2.

Package Size :

200 μg

Uses :

Immunocytochemistry;Immunohistochemistry;Immunoprecipitation;Western Blotting

Storage :

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.