IKKβ Antibody, clone 10AG2 | EMD Millipore 05-535 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

IKKβ Antibody, clone 10AG2

catalog :

05-535

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10AG2

The same clone is also sold as:

Abnova: MAB0075
Invitrogen: MA5-16154
OriGene: TA336374
Active Motif: 40906
Rockland Immunochemicals: 200-301-H83
Novus Biologicals: NBP2-80788, NB100-56509
LifeSpan Biosciences: LS-C554

reactivity :

human, mouse, rat

application :

western blot

citations: 10

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...	Zhang J, MacArtney T, Peggie M, Cohen P. Interleukin-1 and TRAF6-dependent activation of TAK1 in the absence of TAB2 and TAB3. Biochem J. 2017;474:2235-2248 pubmed publisher
western blot; human; loading ...; fig 3g	Strickson S, Emmerich C, Goh E, Zhang J, Kelsall I, MacArtney T, et al. Roles of the TRAF6 and Pellino E3 ligases in MyD88 and RANKL signaling. Proc Natl Acad Sci U S A. 2017;114:E3481-E3489 pubmed publisher
western blot; human; loading ...; fig 5B	Bakshi S, Taylor J, Strickson S, McCartney T, Cohen P. Identification of TBK1 complexes required for the phosphorylation of IRF3 and the production of interferon ?. Biochem J. 2017;474:1163-1174 pubmed publisher
western blot; mouse; fig 4e	Lu J, Chiang Y, Lin Y, Chang Y, Lu C, Chen T, et al. Disruption of Lipid Raft Function Increases Expression and Secretion of Monocyte Chemoattractant Protein-1 in 3T3-L1 Adipocytes. PLoS ONE. 2016;11:e0169005 pubmed publisher
western blot; mouse; loading ...; fig 5j	Vincendeau M, Hadian K, Messias A, Brenke J, Hålander J, Griesbach R, et al. Inhibition of Canonical NF-ÎºB Signaling by a Small Molecule Targeting NEMO-Ubiquitin Interaction. Sci Rep. 2016;6:18934 pubmed publisher
western blot; mouse	Wu Y, Tan H, Huang Q, Sun X, Zhu X, Shen H. zVAD-induced necroptosis in L929 cells depends on autocrine production of TNF? mediated by the PKC-MAPKs-AP-1 pathway. Cell Death Differ. 2011;18:26-37 pubmed publisher
	Lai T, Wu S, Tsai M, Chuang E, Chuang L, Hsu L, et al. Transcription of Tnfaip3 is regulated by NF-?B and p38 via C/EBP? in activated macrophages. PLoS ONE. 2013;8:e73153 pubmed publisher
	Simonin Y, Vegna S, Akkari L, Grégoire D, Antoine E, Piette J, et al. Lymphotoxin signaling is initiated by the viral polymerase in HCV-linked tumorigenesis. PLoS Pathog. 2013;9:e1003234 pubmed publisher
	Hadian K, Griesbach R, Dornauer S, Wanger T, Nagel D, Metlitzky M, et al. NF-?B essential modulator (NEMO) interaction with linear and lys-63 ubiquitin chains contributes to NF-?B activation. J Biol Chem. 2011;286:26107-17 pubmed publisher
	Ruggiero T, Trabucchi M, De Santa F, Zupo S, Harfe B, McManus M, et al. LPS induces KH-type splicing regulatory protein-dependent processing of microRNA-155 precursors in macrophages. FASEB J. 2009;23:2898-908 pubmed publisher

product information

Catalog Number :

05-535

Subcategory :

Cell Signaling

Product Name :

Anti-IKKβ Antibody, clone 10AG2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

O14920

Host Name :

Mouse

Antigen :

IKKβ

Clone :

10AG2

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-IKKβ Antibody, clone 10AG2

Cross Reactivity :

Human;Rat;Mouse

Background :

IKK beta (I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK alpha, IKK beta, IKK gamma and IKAP. Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. IKK beta has been shown to activate NF-kB and phosphorylate IKB alpha and beta. Phosphorylation of 2 sites at the activation loop of IKK beta is essential for activation of IKK by TNF and IL1. Once activated, IKK beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKK beta activity can also be regulated by MEKK-1.

ALT Names :

I-kappa-B kinase 2;I-kappa-B-kinase beta;Nuclear factor NF-kappa-B inhibitor kinase beta;inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta;inhibitor of nuclear factor kappa B kinase beta subunit

Immunogen :

His-tagged, full length human IKKβ/IKK2. Clone: 10AG2.

Specificity :

This antibody recognizes IKKβ/IKK2, Mr 87 kDa.

Package Size :

100 µg

Uses :

Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt.