acetyl-Lysine Antibody, clone 4G12 | EMD Millipore 05-515 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

acetyl-Lysine Antibody, clone 4G12

catalog :

05-515

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4G12

reactivity :

human

application :

western blot, immunoprecipitation

citations: 22

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 2e	Liu X, Tan Y, Zhang C, Zhang Y, Zhang L, Ren P, et al. NAT10 regulates p53 activation through acetylating p53 at K120 and ubiquitinating Mdm2. EMBO Rep. 2016;17:349-66 pubmed publisher
	Hong S, Ng L, Ng L, Inoue T, Tolwinski N, Hagen T, et al. The Role of Mitochondrial Non-Enzymatic Protein Acylation in Ageing. PLoS ONE. 2016;11:e0168752 pubmed publisher
	Ortega Atienza S, Wong V, Deloughery Z, Luczak M, Zhitkovich A. ATM and KAT5 safeguard replicating chromatin against formaldehyde damage. Nucleic Acids Res. 2016;44:198-209 pubmed publisher
	Safronova O, Nakahama K, Morita I. Acute hypoxia affects P-TEFb through HDAC3 and HEXIM1-dependent mechanism to promote gene-specific transcriptional repression. Nucleic Acids Res. 2014;42:8954-69 pubmed publisher
	Wang L, Di L, Noguchi C. AMPK is involved in mediation of erythropoietin influence on metabolic activity and reactive oxygen species production in white adipocytes. Int J Biochem Cell Biol. 2014;54:1-9 pubmed publisher
	Cazzalini O, Sommatis S, Tillhon M, Dutto I, Bachi A, Rapp A, et al. CBP and p300 acetylate PCNA to link its degradation with nucleotide excision repair synthesis. Nucleic Acids Res. 2014;42:8433-48 pubmed publisher
	Ferretta A, Gaballo A, Tanzarella P, Piccoli C, Capitanio N, Nico B, et al. Effect of resveratrol on mitochondrial function: implications in parkin-associated familiar Parkinson's disease. Biochim Biophys Acta. 2014;1842:902-15 pubmed publisher
	Gao B, Xu W, Zhong L, Zhang Q, Su Y, Xiong S. p300, but not PCAF, collaborates with IRF-1 in stimulating TRIM22 expression independently of its histone acetyltransferase activity. Eur J Immunol. 2013;43:2174-84 pubmed publisher
	Banik D, Khan A, Walseng E, Segal B, Abrams S. Interferon regulatory factor-8 is important for histone deacetylase inhibitor-mediated antitumor activity. PLoS ONE. 2012;7:e45422 pubmed publisher
	Tillhon M, Cazzalini O, Nardo T, Necchi D, Sommatis S, Stivala L, et al. p300/CBP acetyl transferases interact with and acetylate the nucleotide excision repair factor XPG. DNA Repair (Amst). 2012;11:844-52 pubmed publisher
	Chuang C, Yu Lee L. Identifying acetylated proteins in mitosis. Methods Mol Biol. 2012;909:181-204 pubmed publisher
	Li D, Dammer E, Sewer M. Resveratrol stimulates cortisol biosynthesis by activating SIRT-dependent deacetylation of P450scc. Endocrinology. 2012;153:3258-68 pubmed publisher
	Chae Y, Kim H, Kim D, Lee H, Lee H. Cell density-dependent acetylation of ?Np63? is associated with p53-dependent cell cycle arrest. FEBS Lett. 2012;586:1128-34 pubmed publisher
	Wang P, Zhang R, Song J, Guan Y, Xu T, Du H, et al. Loss of AMP-activated protein kinase-?2 impairs the insulin-sensitizing effect of calorie restriction in skeletal muscle. Diabetes. 2012;61:1051-61 pubmed publisher
	Tsunoda K, Oikawa H, Tada H, Tatemichi Y, Muraoka S, Miura S, et al. Nucleus accumbens-associated 1 contributes to cortactin deacetylation and augments the migration of melanoma cells. J Invest Dermatol. 2011;131:1710-9 pubmed publisher
	Wu L, Ma C, Zhao Y, Jain A. Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression. J Biol Chem. 2011;286:2236-44 pubmed publisher
	Revenko A, Kalashnikova E, Gemo A, Zou J, Chen H. Chromatin loading of E2F-MLL complex by cancer-associated coregulator ANCCA via reading a specific histone mark. Mol Cell Biol. 2010;30:5260-72 pubmed publisher
	Singh J, Aaronson S, Mlodzik M. Drosophila Abelson kinase mediates cell invasion and proliferation through two distinct MAPK pathways. Oncogene. 2010;29:4033-45 pubmed publisher
	Kuo H, Lee D, Chen C, Liu M, Chou C, Lee H, et al. ARD1 stabilization of TSC2 suppresses tumorigenesis through the mTOR signaling pathway. Sci Signal. 2010;3:ra9 pubmed publisher
	Gonzalez Rodriguez A, Mas Gutierrez J, Sanz González S, Ros M, Burks D, Valverde A. Inhibition of PTP1B restores IRS1-mediated hepatic insulin signaling in IRS2-deficient mice. Diabetes. 2010;59:588-99 pubmed publisher
	Ochocka A, Kampanis P, Nicol S, Allende Vega N, Cox M, Marcar L, et al. FKBP25, a novel regulator of the p53 pathway, induces the degradation of MDM2 and activation of p53. FEBS Lett. 2009;583:621-6 pubmed publisher
	Zhang J. The direct involvement of SirT1 in insulin-induced insulin receptor substrate-2 tyrosine phosphorylation. J Biol Chem. 2007;282:34356-64 pubmed

product information

Catalog Number :

05-515

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-acetyl-Lysine Antibody, clone 4G12

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Mouse

Antigen :

acetyl-Lysine

Clone :

4G12

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-acetyl-Lysine Antibody, clone 4G12

Cross Reactivity :

Mouse;Rat;Human;Vertebrates

Background :

In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation.

Immunogen :

Mixture of chemically acetylated antigens; recognizes numerous proteins acetylated on a lysine, most prominently on histones.

Specificity :

Acetylated lysine-containing proteins including histones. Of all the possible H3 and H4 acetylation sites, this antibody exhibits highest affinity for histone H4 acetylated on lysine 8 and histone H3 acetylated on lysine 14.

Package Size :

100 µg

Uses :

Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.