This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
TCF-4 Antibody, clone 6H5-3
catalog :
05-511
quantity :
200 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
6H5-3

The same clone is also sold as:
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - free floating section
citations: 30
Published Application/Species/Sample/DilutionReference
  • chromatin immunoprecipitation; human; loading ...; fig 1b
Matsumoto S, Yamamichi T, Shinzawa K, Kasahara Y, Nojima S, Kodama T, et al. GREB1 induced by Wnt signaling promotes development of hepatoblastoma by suppressing TGFβ signaling. Nat Commun. 2019;10:3882 pubmed publisher
  • immunohistochemistry; mouse; 1:100; loading ...; fig 2g
van de Vlekkert D, Demmers J, Nguyen X, Campos Y, Machado E, Annunziata I, et al. Excessive exosome release is the pathogenic pathway linking a lysosomal deficiency to generalized fibrosis. Sci Adv. 2019;5:eaav3270 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50; loading ...; fig 8c
  • western blot; human; 1:50; fig s4b
Huang T, Alvarez A, Pangeni R, Horbinski C, Lu S, Kim S, et al. A regulatory circuit of miR-125b/miR-20b and Wnt signalling controls glioblastoma phenotypes through FZD6-modulated pathways. Nat Commun. 2016;7:12885 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:250; loading ...; fig 7b
Szewczyk L, Brozko N, Nagalski A, Röckle I, Werneburg S, Hildebrandt H, et al. ST8SIA2 promotes oligodendrocyte differentiation and the integrity of myelin and axons. Glia. 2017;65:34-49 pubmed publisher
  • immunohistochemistry; mouse; 1:200; loading ...; fig s5a
Marques S, Zeisel A, Codeluppi S, van Bruggen D, Mendanha Falcão A, Xiao L, et al. Oligodendrocyte heterogeneity in the mouse juvenile and adult central nervous system. Science. 2016;352:1326-1329 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; fig 2
Kim H, Kim J, Nam K, Kim W. Clinical significance of midkine expression in sporadic desmoid tumors. Oncol Lett. 2016;11:1677-1684 pubmed
  • western blot; human; fig 2
Katoh I, Fukunishi N, Fujimuro M, Kasai H, Moriishi K, Hata R, et al. Repression of Wnt/β-catenin response elements by p63 (TP63). Cell Cycle. 2016;15:699-710 pubmed publisher
  • chromatin immunoprecipitation; mouse; fig 5
  • western blot; mouse; fig 3
Akhade V, Dighe S, Kataruka S, Rao M. Mechanism of Wnt signaling induced down regulation of mrhl long non-coding RNA in mouse spermatogonial cells. Nucleic Acids Res. 2016;44:387-401 pubmed publisher
  • western blot; human; 1:1000
Velicky P, Haider S, Otti G, Fiala C, Pollheimer J, Knöfler M. Notch-dependent RBPJ? inhibits proliferation of human cytotrophoblasts and their differentiation into extravillous trophoblasts. Mol Hum Reprod. 2014;20:756-66 pubmed publisher
  • chromatin immunoprecipitation; mouse; 5 ug/time
Konsavage W, Yochum G. The myc 3' wnt-responsive element suppresses colonic tumorigenesis. Mol Cell Biol. 2014;34:1659-69 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig st13
Hwang W, Jiang J, Yang S, Huang T, Lan H, Teng H, et al. MicroRNA-146a directs the symmetric division of Snail-dominant colorectal cancer stem cells. Nat Cell Biol. 2014;16:268-80 pubmed publisher
  • chromatin immunoprecipitation; human
  • immunoprecipitation; human
  • western blot; human; 1:1000
Rennoll S, Konsavage W, Yochum G. Nuclear AXIN2 represses MYC gene expression. Biochem Biophys Res Commun. 2014;443:217-22 pubmed publisher
  • chromatin immunoprecipitation; human
  • western blot; human
Latasa M, Salis F, Urtasun R, Garcia Irigoyen O, Elizalde M, Uriarte I, et al. Regulation of amphiregulin gene expression by ?-catenin signaling in human hepatocellular carcinoma cells: a novel crosstalk between FGF19 and the EGFR system. PLoS ONE. 2012;7:e52711 pubmed publisher
  • chromatin immunoprecipitation; mouse
Styner M, Meyer M, Galior K, Case N, Xie Z, Sen B, et al. Mechanical strain downregulates C/EBP? in MSC and decreases endoplasmic reticulum stress. PLoS ONE. 2012;7:e51613 pubmed publisher
Antony L, van der Schoor F, Dalrymple S, Isaacs J. Androgen receptor (AR) suppresses normal human prostate epithelial cell proliferation via AR/?-catenin/TCF-4 complex inhibition of c-MYC transcription. Prostate. 2014;74:1118-31 pubmed publisher
Lade A, Ranganathan S, Luo J, Monga S. Calpain induces N-terminal truncation of ?-catenin in normal murine liver development: diagnostic implications in hepatoblastomas. J Biol Chem. 2012;287:22789-98 pubmed publisher
Konsavage W, Kyler S, Rennoll S, Jin G, Yochum G. Wnt/?-catenin signaling regulates Yes-associated protein (YAP) gene expression in colorectal carcinoma cells. J Biol Chem. 2012;287:11730-9 pubmed publisher
Satow R, Shitashige M, Jigami T, Fukami K, Honda K, Kitabayashi I, et al. ?-catenin inhibits promyelocytic leukemia protein tumor suppressor function in colorectal cancer cells. Gastroenterology. 2012;142:572-81 pubmed publisher
Lan F, Yue X, Han L, Shi Z, Yang Y, Pu P, et al. Genome-wide identification of TCF7L2/TCF4 target miRNAs reveals a role for miR-21 in Wnt-driven epithelial cancer. Int J Oncol. 2012;40:519-26 pubmed publisher
Pourebrahim R, Houtmeyers R, Ghogomu S, Janssens S, Thelie A, Tran H, et al. Transcription factor Zic2 inhibits Wnt/?-catenin protein signaling. J Biol Chem. 2011;286:37732-40 pubmed publisher
Murphy M, Lawson J, Mathew S, Hutcheson D, Kardon G. Satellite cells, connective tissue fibroblasts and their interactions are crucial for muscle regeneration. Development. 2011;138:3625-37 pubmed publisher
Bordonaro M, Tewari S, Atamna W, Lazarova D. The Notch ligand Delta-like 1 integrates inputs from TGFbeta/Activin and Wnt pathways. Exp Cell Res. 2011;317:1368-81 pubmed publisher
Zhao J, Schug J, Li M, Kaestner K, Grant S. Disease-associated loci are significantly over-represented among genes bound by transcription factor 7-like 2 (TCF7L2) in vivo. Diabetologia. 2010;53:2340-6 pubmed publisher
Zhao B, Schlesiger C, Masucci M, Lindsten K. The ubiquitin specific protease 4 (USP4) is a new player in the Wnt signalling pathway. J Cell Mol Med. 2009;13:1886-95 pubmed publisher
Wright J, Brown S, Cole M. Upregulation of c-MYC in cis through a large chromatin loop linked to a cancer risk-associated single-nucleotide polymorphism in colorectal cancer cells. Mol Cell Biol. 2010;30:1411-20 pubmed publisher
Beildeck M, Islam M, Shah S, Welsh J, Byers S. Control of TCF-4 expression by VDR and vitamin D in the mouse mammary gland and colorectal cancer cell lines. PLoS ONE. 2009;4:e7872 pubmed publisher
Gan X, Wang J, Xi Y, Wu Z, Li Y, Li L. Nuclear Dvl, c-Jun, beta-catenin, and TCF form a complex leading to stabilization of beta-catenin-TCF interaction. J Cell Biol. 2008;180:1087-100 pubmed publisher
Yamada M, Ohnishi J, Ohkawara B, Iemura S, Satoh K, Hyodo Miura J, et al. NARF, an nemo-like kinase (NLK)-associated ring finger protein regulates the ubiquitylation and degradation of T cell factor/lymphoid enhancer factor (TCF/LEF). J Biol Chem. 2006;281:20749-60 pubmed
Sato S, Idogawa M, Honda K, Fujii G, Kawashima H, Takekuma K, et al. Beta-catenin interacts with the FUS proto-oncogene product and regulates pre-mRNA splicing. Gastroenterology. 2005;129:1225-36 pubmed
Gottardi C, Gumbiner B. Distinct molecular forms of beta-catenin are targeted to adhesive or transcriptional complexes. J Cell Biol. 2004;167:339-49 pubmed
product information
Catalog Number :
05-511
Subcategory :
Epigenetics & Nuclear Function
Product Name :
Anti-TCF-4 Antibody, clone 6H5-3
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
Q9NQB0
Host Name :
Mouse
Antigen :
TCF-4
Clone :
6H5-3
Conjugate :
Purified
Isotype :
IgG2a
Product Description :
Anti-TCF-4 Antibody, clone 6H5-3
Cross Reactivity :
Human;Mouse
Background :
TCF4, also known as TCF7L2, is expressed widely during development. Gene targeting study indicates that it is required to maintain the crypt stem cells of the small intestine. TCF4 has many different splicing isoforms and they are expressed differentially in tissues and in cancers of different stages. Studies also indicate that variant of the TCF4 gene confers an increased risk of type 2 diabetes.
ALT Names :
HMG box transcription factor 4;Transcription factor 7-like 2;T-Cell factor 4;T-cell-specific, HMG box;T-Cell-specific transcription factor 4;Transcription factor 7-like 2
Immunogen :
Amino acids 31-331 of human TCF-4.
Specificity :
This antibody recognizes TCF-4
Package Size :
200 µg
Uses :
Electrophoretic Mobility Shift Assay;Immunocytochemistry;Immunohistochemistry;Immunoprecipitation;Western Blotting
Storage :
Stable for 1 year at -20ºC from date of receipt.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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