Cdc25C Antibody, clone TC-15 | EMD Millipore 05-507 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Cdc25C Antibody, clone TC-15

catalog :

05-507

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

TC-15

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 3c	Lee J, Sung J, Choi E, Yoon H, Kang B, Hong E, et al. C/EBPβ Is a Transcriptional Regulator of Wee1 at the G₂/M Phase of the Cell Cycle. Cells. 2019;8: pubmed publisher

product information

Catalog Number :

05-507

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Cdc25C Antibody, clone TC-15

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P30307

Host Name :

Mouse

Antigen :

Cdc25C

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-Cdc25C Antibody, clone TC-15

Cross Reactivity :

Human;Mouse

Background :

The activity of cyclin-dependent kinases is regulated by their phosphorylation status, which is controlled by the antagonistic action of CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B and CDC25C. CDC25C is a ~56 kDa dual specific protein phosphatase that controls entry into mitosis by regulating the dephosphorylation of the Cdk1/cyclin B complex. Phosphorylation of Cdc25C creates a binding site for 14-3-3 protein which inhibits Cdc25C. This prevents activation of the Cdk1/cyclin B complex and prevents mitotic entry. Cdc25C is phosphorylated by checkpoint kinases throughout interphase but becomes dephosphorylated during mitosis. Cdc25C is also regulated by p53 via a p53 response element in its promoter, and it is predominantly expressed in the G2 phase of the cell cycle.

Immunogen :

GST fusion-protein corresponding to residues 1-258 of human cdc25C. Epitope mapped to residues 222-246.

Specificity :

cdc25C

Package Size :

100 µg

Uses :

Immunoprecipitation;Western Blotting;Immunocytochemistry

Storage :

2 years at -20°C