Histone H3 Antibody, clone 6.6.2 | EMD Millipore 05-499 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Histone H3 Antibody, clone 6.6.2

catalog :

05-499

quantity :

200 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6.6.2

reactivity :

fission yeast, human, mouse, rat, fruit fly

application :

western blot, chromatin immunoprecipitation, immunohistochemistry - paraffin section, western blot knockout validation

citations: 29

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; human; fig 5	Helmke C, Raab M, Rodel F, Matthess Y, Oellerich T, Mandal R, et al. Ligand stimulation of CD95 induces activation of Plk3 followed by phosphorylation of caspase-8. Cell Res. 2016;26:914-34 pubmed publisher
western blot; mouse; 1:1000; fig 2c	Wang S, Ko S, Kim Y, Jo S, Lee S, Jung S, et al. Capsaicin upregulates HDAC2 via TRPV1 and impairs neuronal maturation in mice. Exp Mol Med. 2018;50:e455 pubmed publisher
western blot; mouse; 1:10,000; loading ...; fig 2a	Kasowitz S, Luo M, Ma J, Leu N, Wang P. Embryonic lethality and defective male germ cell development in mice lacking UTF1. Sci Rep. 2017;7:17259 pubmed publisher
western blot; mouse; loading ...; fig 2e	Ueda T, Nakata Y, Nagamachi A, Yamasaki N, Kanai A, Sera Y, et al. Propagation of trimethylated H3K27 regulated by polycomb protein EED is required for embryogenesis, hematopoietic maintenance, and tumor suppression. Proc Natl Acad Sci U S A. 2016;113:10370-5 pubmed publisher
western blot; human; 1:1000; fig 5	Passerini V, Ozeri Galai E, de Pagter M, Donnelly N, Schmalbrock S, Kloosterman W, et al. The presence of extra chromosomes leads to genomic instability. Nat Commun. 2016;7:10754 pubmed publisher
chromatin immunoprecipitation; human; fig 5	Stein C, Nötzold R, Riedl S, Bouchard C, Bauer U. The Arginine Methyltransferase PRMT6 Cooperates with Polycomb Proteins in Regulating HOXA Gene Expression. PLoS ONE. 2016;11:e0148892 pubmed publisher
western blot; human; 1:1000	Vossaert L, Meert P, Scheerlinck E, Glibert P, Van Roy N, Heindryckx B, et al. Identification of histone H3 clipping activity in human embryonic stem cells. Stem Cell Res. 2014;13:123-34 pubmed publisher
western blot; rat; 1:1000	Patki G, Solanki N, Atrooz F, Ansari A, Allam F, Jannise B, et al. Novel mechanistic insights into treadmill exercise based rescue of social defeat-induced anxiety-like behavior and memory impairment in rats. Physiol Behav. 2014;130:135-44 pubmed publisher
immunohistochemistry - paraffin section; fruit fly	Mohan R, Dialynas G, Weake V, Liu J, Martin Brown S, Florens L, et al. Loss of Drosophila Ataxin-7, a SAGA subunit, reduces H2B ubiquitination and leads to neural and retinal degeneration. Genes Dev. 2014;28:259-72 pubmed publisher
western blot; mouse	Valor L, Guiretti D, Lopez Atalaya J, Barco A. Genomic landscape of transcriptional and epigenetic dysregulation in early onset polyglutamine disease. J Neurosci. 2013;33:10471-82 pubmed publisher
western blot; mouse	Schwab K, Smith G, Dressler G. Arrested spermatogenesis and evidence for DNA damage in PTIP mutant testes. Dev Biol. 2013;373:64-71 pubmed publisher
western blot; fission yeast	Koch A, Rode H, Richters A, Rauh D, Hauf S. A chemical genetic approach for covalent inhibition of analogue-sensitive aurora kinase. ACS Chem Biol. 2012;7:723-31 pubmed publisher
western blot; mouse	Foster C, Dovey O, Lezina L, Luo J, Gant T, Barlev N, et al. Lysine-specific demethylase 1 regulates the embryonic transcriptome and CoREST stability. Mol Cell Biol. 2010;30:4851-63 pubmed publisher
western blot; mouse	Cherrington B, Morency E, Struble A, Coonrod S, Wakshlag J. Potential role for peptidylarginine deiminase 2 (PAD2) in citrullination of canine mammary epithelial cell histones. PLoS ONE. 2010;5:e11768 pubmed publisher
	Gudkova D, Dergai O, Praz V, Herr W. HCF-2 inhibits cell proliferation and activates differentiation-gene expression programs. Nucleic Acids Res. 2019;47:5792-5808 pubmed publisher
	Yang L, Zha Y, Ding J, Ye B, Liu M, Yan C, et al. Histone demethylase KDM6B has an anti-tumorigenic function in neuroblastoma by promoting differentiation. Oncogenesis. 2019;8:3 pubmed publisher
	Yang C, Shen W, Chen H, Chu L, Xu Y, Zhou X, et al. Characterization and subcellular localization of histone deacetylases and their roles in response to abiotic stresses in soybean. BMC Plant Biol. 2018;18:226 pubmed publisher
	Soleimani V, Nguyen D, Ramachandran P, Palidwor G, Porter C, Yin H, et al. Cis-regulatory determinants of MyoD function. Nucleic Acids Res. 2018;46:7221-7235 pubmed publisher
	Lee J, Yun J, Zhao W, Shen W, Amasino R. A methyltransferase required for proper timing of the vernalization response in Arabidopsis. Proc Natl Acad Sci U S A. 2015;112:2269-74 pubmed publisher
	Bastianelli D, Siciliano C, Puca R, Coccia A, Murdoch C, Bordin A, et al. Influence of Egr-1 in cardiac tissue-derived mesenchymal stem cells in response to glucose variations. Biomed Res Int. 2014;2014:254793 pubmed publisher
	Wu Z, Ma C, Shan Z, Ju Y, Li S, Zhao Q. Histone deacetylase inhibitors suppress the growth of human osteosarcomas in vitro and in vivo. J BUON. 2013;18:1032-7 pubmed
	Osumek J, Revesz A, Morton J, Davidge S, Hardy D. Enhanced trimethylation of histone h3 mediates impaired expression of hepatic glucose 6-phosphatase expression in offspring from rat dams exposed to hypoxia during pregnancy. Reprod Sci. 2014;21:112-21 pubmed publisher
	Kim W, Latrasse D, Servet C, Zhou D. Arabidopsis histone deacetylase HDA9 regulates flowering time through repression of AGL19. Biochem Biophys Res Commun. 2013;432:394-8 pubmed publisher
	Wang J, Leung J, Gong Z, Feng L, Shi X, Chen J. PHF6 regulates cell cycle progression by suppressing ribosomal RNA synthesis. J Biol Chem. 2013;288:3174-83 pubmed publisher
	Ratzka A, Baron O, Stachowiak M, Grothe C. Fibroblast growth factor 2 regulates dopaminergic neuron development in vivo. J Neurochem. 2012;122:94-105 pubmed publisher
	Di Paola D, Zannis Hadjopoulos M. Comparative analysis of pre-replication complex proteins in transformed and normal cells. J Cell Biochem. 2012;113:1333-47 pubmed publisher
	Ahmad A, Dong Y, Cao X. Characterization of the PRMT gene family in rice reveals conservation of arginine methylation. PLoS ONE. 2011;6:e22664 pubmed publisher
	Widiez T, El Kafafi E, Girin T, Berr A, Ruffel S, Krouk G, et al. High nitrogen insensitive 9 (HNI9)-mediated systemic repression of root NO3- uptake is associated with changes in histone methylation. Proc Natl Acad Sci U S A. 2011;108:13329-34 pubmed publisher
	Gomez Pinilla F, Zhuang Y, Feng J, Ying Z, Fan G. Exercise impacts brain-derived neurotrophic factor plasticity by engaging mechanisms of epigenetic regulation. Eur J Neurosci. 2011;33:383-90 pubmed publisher

product information

Catalog Number :

05-499

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Histone H3 Antibody, clone 6.6.2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16695

Host Name :

Mouse

Antigen :

Histone H3

Clone :

6.6.2

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-Histone H3 Antibody, clone 6.6.2

Cross Reactivity :

Vertebrates;Green Plants;Human

Background :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

ALT Names :

H3 histone, family 3A

Immunogen :

KLH conjugated peptide corresponding to amino acids 1-20 [ARTKQTARKSTGGKAPRKQLC] of human histone H3. Clone 6.6.2.

Specificity :

This antibody recognizes and is specific for histone H3, Mr 17kDa.

Package Size :

200 µg

Uses :

Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.