phospho-STAT5A/B (Tyr694/699) Antibody, clone 8-5-2 | EMD Millipore 05-495 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-STAT5A/B (Tyr694/699) Antibody, clone 8-5-2

catalog :

05-495

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

8-5-2

reactivity :

African green monkey, human, mouse

application :

western blot

citations: 11

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig 2a	Stephens J, Bailey J, Hang H, Rittell V, Dietrich M, Mynatt R, et al. Adipose Tissue Dysfunction Occurs Independently of Obesity in Adipocyte-Specific Oncostatin Receptor Knockout Mice. Obesity (Silver Spring). 2018;26:1439-1447 pubmed publisher
western blot; mouse; 1:1000; fig 5	Takabatake Y, Oxvig C, Nagi C, Adelson K, Jaffer S, Schmidt H, et al. Lactation opposes pappalysin-1-driven pregnancy-associated breast cancer. EMBO Mol Med. 2016;8:388-406 pubmed publisher
western blot; human	Elliott S, Swift S, Busse L, Scully S, Van G, Rossi J, et al. Epo receptors are not detectable in primary human tumor tissue samples. PLoS ONE. 2013;8:e68083 pubmed publisher
western blot; African green monkey; 1:4000	Varco Merth B, Mirza K, Alzhanov D, Chia D, Rotwein P. Biochemical characterization of diverse Stat5b-binding enhancers that mediate growth hormone-activated insulin-like growth factor-I gene transcription. PLoS ONE. 2012;7:e50278 pubmed publisher
	Ushiki T, Huntington N, Glaser S, Kiu H, Georgiou A, Zhang J, et al. Rapid Inflammation in Mice Lacking Both SOCS1 and SOCS3 in Hematopoietic Cells. PLoS ONE. 2016;11:e0162111 pubmed publisher
	Sanz Moreno A, Fuhrmann D, Wolf E, von Eyss B, Eilers M, Elsässer H. Miz1 deficiency in the mammary gland causes a lactation defect by attenuated Stat5 expression and phosphorylation. PLoS ONE. 2014;9:e89187 pubmed publisher
	Guigon C, Kim D, Willingham M, Cheng S. Mutation of thyroid hormone receptor-β in mice predisposes to the development of mammary tumors. Oncogene. 2011;30:3381-90 pubmed publisher
	Elling C, Erben P, Walz C, Frickenhaus M, Schemionek M, Stehling M, et al. Novel imatinib-sensitive PDGFRA-activating point mutations in hypereosinophilic syndrome induce growth factor independence and leukemia-like disease. Blood. 2011;117:2935-43 pubmed publisher
	Wolf A, Eller K, Zeiser R, Dürr C, Gerlach U, Sixt M, et al. The sphingosine 1-phosphate receptor agonist FTY720 potently inhibits regulatory T cell proliferation in vitro and in vivo. J Immunol. 2009;183:3751-60 pubmed publisher
	Garinis G, Uittenboogaard L, Stachelscheid H, Fousteri M, van Ijcken W, Breit T, et al. Persistent transcription-blocking DNA lesions trigger somatic growth attenuation associated with longevity. Nat Cell Biol. 2009;11:604-15 pubmed publisher
	Uchida K, Nakata K, Suzuki T, Luisetti M, Watanabe M, Koch D, et al. Granulocyte/macrophage-colony-stimulating factor autoantibodies and myeloid cell immune functions in healthy subjects. Blood. 2009;113:2547-56 pubmed publisher

product information

Catalog Number :

05-495

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone 8-5-2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P51692

Host Name :

Mouse

Antigen :

phospho-STAT5A/B (Tyr694/699)

Clone :

clone 8-5-2

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone 8-5-2

Cross Reactivity :

Human;Mouse;Rat;Bovine;Sheep

Background :

STAT (Signal Transducers and Activators of Transcription) are a family of conserved transcription factors that transduce high-fidelity signals for the cytokine family of ligands and receptors as the principle substrates of JAK kinases (1,2). In resting cells, the STATs exist in an inactive state in the cytoplasm until activated. Activators include cytokines, growth factors, and some peptides. These activate JAK kinases, which when activated, bind to the STAT SH2 (Src homology domain-2) domain resulting in phosphorylation of STAT (1). Upon activation, STAT1, -3, -4, -5A, and -5B all form homodimers. STAT1 and STAT2, as well as STAT1 and STAT3 form heterodimers, and several STATs form tetramers, depending on the variables of the activating ligand (1). After dimerization, the STATs accumulate in the nucleus by way of nuclear transport (1). STAT5A and STAT5B appear to be most important to the function of growth hormone and prolactin and play critical roles in milk production (1,2,3). The phosphorylation of Tyrosine 694/699 is a hallmark of activated STAT5.

ALT Names :

signal transducer and activator of transcription 5A

Immunogen :

KLH-conjugated, synthetic phosphopeptide (KAVDG[pY]VKPQIK) corresponding to amino acids 689-700 of STAT5A and 694-705 of STAT5B. Clone 8-5-2.

Specificity :

Specific for the phosphorylated tyrosine residue 694 of STAT5A (94kDa) and 699 of STAT5B (92kDa). Unidentified proteins may be detected in some lysates.

Package Size :

100 μg

Uses :

Western Blotting

Storage :

Stable for 1 year at from date of receipt.