phospho-STAT3 (Tyr705) Antibody, clone 9E12 | EMD Millipore 05-485 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-STAT3 (Tyr705) Antibody, clone 9E12

catalog :

05-485

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

9E12

The same clone is also sold as:

Santa Cruz Biotechnology: sc-81523
Nanotools: 0036-100/STAT3-9E12
Enzo Life Sciences: ALX-804-180-C100

reactivity :

human, mouse, dogs

application :

western blot, ELISA, immunoprecipitation, other

citations: 4

Published Application/Species/Sample/Dilution	Reference
other; mouse; 1:10,000; fig s1	Chiang C, Uzoma I, Lane D, MemiÅ¡eviÄ‡ V, Alem F, Yao K, et al. A reverse-phase protein microarray-based screen identifies host signaling dynamics upon Burkholderia spp. infection. Front Microbiol. 2015;6:683 pubmed publisher
	Hong S, Yang S, Kim T, Shim J, Lee H, Lee G, et al. Molecular integration of HoxB4 and STAT3 for self-renewal of hematopoietic stem cells: a model of molecular convergence for stemness. Stem Cells. 2014;32:1313-22 pubmed publisher
	Cheng P, Lin Y, Chen Y, Lee Y, Tai C, Wang Y, et al. Interplay between SIN3A and STAT3 mediates chromatin conformational changes and GFAP expression during cellular differentiation. PLoS ONE. 2011;6:e22018 pubmed publisher
	Oufkir T, Arseneault M, Sanderson J, Vaillancourt C. The 5-HT 2A serotonin receptor enhances cell viability, affects cell cycle progression and activates MEK-ERK1/2 and JAK2-STAT3 signalling pathways in human choriocarcinoma cell lines. Placenta. 2010;31:439-47 pubmed publisher

product information

Catalog Number :

05-485

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-phospho-STAT3 (Tyr705) Antibody, clone 9E12

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P40763

Host Name :

Mouse

Antigen :

phospho-STAT3 (Tyr705)

Clone :

9E12

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-phospho-STAT3 (Tyr705) Antibody, clone 9E12

Cross Reactivity :

Human;Mouse;Canine

Background :

STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT1 and STAT2) which were involved in interferon-alpha (IFN-alpha) and IFN-gamma signal transduction. Since then, several additional STAT proteins have been identified (STAT3, 4, 5a, 5b, and 6). STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. In some cases this process is mediated by JAK Kinases (Janus Kinases 1, 2, and 3). For maximum activation of these proteins, phosphorylation at specific tyrosine and serine residues may be required in STAT1 alpha, 3, 4, and 5. Specific functions of the various members of the STAT family are poorly understood. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.

ALT Names :

Acute-phase response factor;DNA-binding protein APRF;signal transducer and activator of transcription 3;signal transducer and activator of transcription 3 (acute-phase response factor)

Immunogen :

KLH-conjugated phosphopeptide containing the AP[pY]LK motif of STAT3

Specificity :

This antibody recognizes STAT3, Mr 92 kDa, phos-phorylated on tyrosine-705. Clone 9E12.

Package Size :

100 µg

Uses :

ELISA;Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.