phospho-MAP Kinase1/2 Antibody | EMD Millipore 05-481 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-MAP Kinase1/2 Antibody

catalog :

05-481

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

12D4

The same clone is also sold as:

Santa Cruz Biotechnology: sc-81492
OriGene: AM00084FC-N, AM00084BT-N, AM00084PU-N

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, immunohistochemistry - free floating section

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 1a western blot; mouse; fig 1b	O Sullivan C, Schubart A, Mir A, Dev K. The dual S1PR1/S1PR5 drug BAF312 (Siponimod) attenuates demyelination in organotypic slice cultures. J Neuroinflammation. 2016;13:31 pubmed publisher
immunohistochemistry - free floating section; rat; 1:100 western blot; rat; 1:500	Taylor D, Moser R, Regulier E, Breuillaud L, Dixon M, Beesen A, et al. MAP kinase phosphatase 1 (MKP-1/DUSP1) is neuroprotective in Huntington's disease via additive effects of JNK and p38 inhibition. J Neurosci. 2013;33:2313-25 pubmed publisher
	Seredenina T, Gokce O, Luthi Carter R. Decreased striatal RGS2 expression is neuroprotective in Huntington's disease (HD) and exemplifies a compensatory aspect of HD-induced gene regulation. PLoS ONE. 2011;6:e22231 pubmed publisher
	Oufkir T, Arseneault M, Sanderson J, Vaillancourt C. The 5-HT 2A serotonin receptor enhances cell viability, affects cell cycle progression and activates MEK-ERK1/2 and JAK2-STAT3 signalling pathways in human choriocarcinoma cell lines. Placenta. 2010;31:439-47 pubmed publisher
	Dormoy V, Danilin S, Lindner V, Thomas L, Rothhut S, Coquard C, et al. The sonic hedgehog signaling pathway is reactivated in human renal cell carcinoma and plays orchestral role in tumor growth. Mol Cancer. 2009;8:123 pubmed publisher

product information

Catalog Number :

05-481

Subcategory :

Cell Signaling

Product Name :

Anti-phospho-MAP Kinase1/2 Antibody

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P27361

Host Name :

Mouse

Antigen :

phospho-MAP Kinase1/2

Clone :

clone 12D4

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-phospho-MAP Kinase1/2 Antibody

Cross Reactivity :

Human;Mouse;Rat;Avian;Canine

Background :

The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase. The ERK1/2 signaling cascade has been shown to be a critical regulator of cell differentiation, cell physiology and neuronal function. Aberrant control of ERK1/2 activity has been implicated in a variety of pathological conditions, including cancer and autoimmune diseases, and efficient study methods are in demand. Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.

ALT Names :

Extracellular signal-regulated kinase;Insulin-stimulated MAP2 kinase;MAP kinase 1;MAPK 1;Microtubule-associated protein 2 kinase;extracellular signal-related kinase 1;mitogen-activated protein kinase 3

Immunogen :

KLH-conjugated phosphopeptide containing the pTEpY motif of Erk1 and Erk2. Clone: 12D4

Specificity :

Specific for phosphorylated (activated) Erk1 and Erk2. Antibody recognition requires phosphorylation of both the threonine and tyrosine residues of Erk1/Erk2 and will not interact with the non-phosphorylated form of the proteins. Does not cross react with activated SAP Kinase 1 or 2.

Package Size :

100 μg

Uses :

Western Blotting;Immunoprecipitation;ELISA;Immunocytochemistry

Storage :

Stable for 1 year at from date of receipt.