Eck/EphA2 Antibody, clone D7 | EMD Millipore 05-480 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Eck/EphA2 Antibody, clone D7

catalog :

05-480

quantity :

200 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

D7

The same clone is also sold as:

MilliporeSigma: E1026
LifeSpan Biosciences: LS-C128748

reactivity :

human, mouse, dogs, bovine

application :

western blot, immunocytochemistry, immunoprecipitation

citations: 9

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; human; loading ...; fig 1b immunocytochemistry; human; 1:200; loading ...; fig 3e western blot; human; 1:1000; loading ...; fig 1b	Gundry C, Marco S, Rainero E, Miller B, Dornier E, Mitchell L, et al. Phosphorylation of Rab-coupling protein by LMTK3 controls Rab14-dependent EphA2 trafficking to promote cell:cell repulsion. Nat Commun. 2017;8:14646 pubmed publisher
western blot; human; loading ...; fig 1c	Neill T, Buraschi S, Goyal A, Sharpe C, Natkanski E, Schaefer L, et al. EphA2 is a functional receptor for the growth factor progranulin. J Cell Biol. 2016;215:687-703 pubmed
immunoprecipitation; human; loading ...; fig 3f western blot; human; loading ...; fig 3d	Barquilla A, Lamberto I, Noberini R, Heynen Genel S, Brill L, Pasquale E. Protein kinase A can block EphA2 receptor-mediated cell repulsion by increasing EphA2 S897 phosphorylation. Mol Biol Cell. 2016;27:2757-70 pubmed publisher
western blot; human; 1:1000; fig s4	Amato K, Wang S, Tan L, Hastings A, Song W, Lovly C, et al. EPHA2 Blockade Overcomes Acquired Resistance to EGFR Kinase Inhibitors in Lung Cancer. Cancer Res. 2016;76:305-18 pubmed publisher
western blot; human	Sato M, Kadota M, Tang B, Yang H, Yang Y, Shan M, et al. An integrated genomic approach identifies persistent tumor suppressive effects of transforming growth factor-? in human breast cancer. Breast Cancer Res. 2014;16:R57 pubmed publisher
western blot; human; 1:1000	Amato K, Wang S, Hastings A, Youngblood V, Santapuram P, Chen H, et al. Genetic and pharmacologic inhibition of EPHA2 promotes apoptosis in NSCLC. J Clin Invest. 2014;124:2037-49 pubmed publisher
immunoprecipitation; human	Sáinz Jaspeado M, Huertas Martínez J, Lagares Tena L, Martín Liberal J, Mateo Lozano S, de Alava E, et al. EphA2-induced angiogenesis in ewing sarcoma cells works through bFGF production and is dependent on caveolin-1. PLoS ONE. 2013;8:e71449 pubmed publisher
	Tiwari A, Schneider M, Fiorino A, Haider R, Okoniewski M, Roschitzki B, et al. Early insights into the function of KIAA1199, a markedly overexpressed protein in human colorectal tumors. PLoS ONE. 2013;8:e69473 pubmed publisher
	Nair P, Salaita K, Petit R, Groves J. Using patterned supported lipid membranes to investigate the role of receptor organization in intercellular signaling. Nat Protoc. 2011;6:523-39 pubmed publisher

product information

Catalog Number :

05-480

Subcategory :

Neuroscience

Product Name :

Anti-Eck/EphA2 Antibody, clone D7

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P29317

Host Name :

Mouse

Antigen :

Eck/EphA2

Clone :

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Eck/EphA2 Antibody, clone D7

Cross Reactivity :

Human;Rat;Mouse;Bovine;Canine

Background :

EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation.

ALT Names :

EPH receptor A2;Epithelial cell kinase;Tyrosine-protein kinase receptor ECK;ephrin receptor EphA2;epithelial cell receptor protein tyrosine kinase;protein tyrosine kinase;receptor protein tyrosine kinase regulated by p53 and E2F-1

Immunogen :

Native protein isolated by purification of phosphotyrosine-containing proteins. Clone D7.

Specificity :

This antibody recognizes Epithelial Cell Kinase (Eck/EphA2), Mr 140 kDa

Package Size :

200 µg

Uses :

Immunocytochemistry;Immunoprecipitation;Enzyme Assay;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. NOTE: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.