GABA A Receptor β2/3 Antibody | EMD Millipore 05-474 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

GABA A Receptor β2/3 Antibody

catalog :

05-474

quantity :

200 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

62-3G1

The same clone is also sold as:

Developmental Studies Hybridoma Bank: 62-3G1

reactivity :

human, mouse, rat, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

citations: 14

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; loading ...; fig 4a western blot; mouse; loading ...; fig 1	Martenson J, Yamasaki T, Chaudhury N, Albrecht D, Tomita S. Assembly rules for GABAA receptor complexes in the brain. elife. 2017;6: pubmed publisher
western blot; human; loading ...; fig 3a	Hernandez C, Kong W, Hu N, Zhang Y, Shen W, Jackson L, et al. Altered Channel Conductance States and Gating of GABAA Receptors by a Pore Mutation Linked to Dravet Syndrome. Eneuro. 2017;4: pubmed publisher
western blot; mouse; 1:300	Arain F, Zhou C, Ding L, Zaidi S, Gallagher M. The developmental evolution of the seizure phenotype and cortical inhibition in mouse models of juvenile myoclonic epilepsy. Neurobiol Dis. 2015;82:164-175 pubmed publisher
western blot; rat; 1:200	Peng X, Hughes E, Moscato E, Parsons T, Dalmau J, Balice Gordon R. Cellular plasticity induced by anti-Î±-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor encephalitis antibodies. Ann Neurol. 2015;77:381-98 pubmed publisher
immunocytochemistry; rat; 2 ug/ml	Swanwick C, Murthy N, Mtchedlishvili Z, Sieghart W, Kapur J. Development of gamma-aminobutyric acidergic synapses in cultured hippocampal neurons. J Comp Neurol. 2006;495:497-510 pubmed
	Moscato E, Peng X, Jain A, Parsons T, Dalmau J, Balice Gordon R. Acute mechanisms underlying antibody effects in anti-N-methyl-D-aspartate receptor encephalitis. Ann Neurol. 2014;76:108-19 pubmed publisher
	Todd E, Gurba K, Botzolakis E, Stanic A, Macdonald R. GABAA receptor biogenesis is impaired by the ?2 subunit febrile seizure-associated mutation, GABRG2(R177G). Neurobiol Dis. 2014;69:215-24 pubmed publisher
	Gonzalez M, Cruz Del Angel Y, Brooks Kayal A. Down-regulation of gephyrin and GABAA receptor subunits during epileptogenesis in the CA1 region of hippocampus. Epilepsia. 2013;54:616-24 pubmed publisher
	Gonzalez C, Moss S, Olsen R. Ethanol promotes clathrin adaptor-mediated endocytosis via the intracellular domain of ?-containing GABAA receptors. J Neurosci. 2012;32:17874-81 pubmed publisher
	Oberlander J, Porter D, Onakomaiya M, Penatti C, Vithlani M, Moss S, et al. Estrous cycle variations in GABA(A) receptor phosphorylation enable rapid modulation by anabolic androgenic steroids in the medial preoptic area. Neuroscience. 2012;226:397-410 pubmed publisher
	Fu X, Rekow S, Spindel E. The ly-6 protein, lynx1, is an endogenous inhibitor of nicotinic signaling in airway epithelium. Am J Physiol Lung Cell Mol Physiol. 2012;303:L661-8 pubmed publisher
	Batalha V, Pego J, Fontinha B, Costenla A, Valadas J, Baqi Y, et al. Adenosine A(2A) receptor blockade reverts hippocampal stress-induced deficits and restores corticosterone circadian oscillation. Mol Psychiatry. 2013;18:320-31 pubmed publisher
	Dufour A, Tell F, Kessler J, Baude A. Mixed GABA-glycine synapses delineate a specific topography in the nucleus tractus solitarii of adult rat. J Physiol. 2010;588:1097-115 pubmed publisher
	Belichenko P, Kleschevnikov A, Masliah E, Wu C, Takimoto Kimura R, Salehi A, et al. Excitatory-inhibitory relationship in the fascia dentata in the Ts65Dn mouse model of Down syndrome. J Comp Neurol. 2009;512:453-66 pubmed publisher

product information

Catalog Number :

05-474

Subcategory :

Neuroscience

Product Name :

Anti-GABA A Receptor β2/3 Antibody

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P14867

Host Name :

Mouse

Antigen :

GABA A Receptor β2/3

Clone :

clone 62-3G1

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-GABA A Receptor β2/3 Antibody

Cross Reactivity :

Bovine;Human;Rat

Background :

The neurotransmitter GABA (gamma-aminobutyric acid) binds to specific transmembrane receptors in the plasma membrane of both pre- and postsynaptic neurons. Three general classes of GABA receptors are known: GABAA and GABAC (ionotropic) receptors and GABAB (metabotropic) receptors. The GABAA (gamma-Aminobutyric Acid type A) receptor is a multiprotein complex that acts as a ligand-gated chloride channel. These receptors are hetero-oligomers, for which 6 subunits are currently known (a, b, g, d, e and r). There are at least 3 different isoforms of the beta subunit, and receptor subunit expression varies during development. Additionally, GABAA receptors are important in regulating the reinforcing properties of alcohol, and expression of the alpha1 and beta 2/3 subunits increase upon chronic exposure to alcohol, and may be potential therapeutic targets for alcohol dependency. The beta 2 subunit is required for receptor assembly and surface expression, while mice with an inactivated beta3 subunit exhibit epilepsy and cleft palate.

ALT Names :

GABA(A) receptor subunit alpha-1;gamma-aminobutyric acid (GABA) A receptor, alpha 1;gamma-aminobutyric acid A receptor, alpha 1

Immunogen :

Affinity-purified GABAA receptor isolated from bovine brain. Clone 62-3G1

Specificity :

Specific for the GABAA Receptor β2 subunit, MW ~55 kDa and β3 subunit, MW ~57 kDa.

Package Size :

200 μg

Uses :

Western Blotting;Immunoprecipitation;Immunohistochemistry

Storage :

Stable for 1 year at 2°C to 8°C from date of receipt.