erbB-3/HER-3 Antibody, clone H3.105.5 | EMD Millipore 05-471 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

erbB-3/HER-3 Antibody, clone H3.105.5

catalog :

05-471

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

H3.105.5

The same clone is also sold as:

LifeSpan Biosciences: LS-C88048

reactivity :

human, mouse

application :

western blot, immunocytochemistry, neutralization, immunoprecipitation

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 4b	Ebbing E, Medema J, Damhofer H, Meijer S, Krishnadath K, van Berge Henegouwen M, et al. ADAM10-mediated release of heregulin confers resistance to trastuzumab by activating HER3. Oncotarget. 2016;7:10243-54 pubmed publisher
immunocytochemistry; human	Kalendová A, Kalasová I, Yamazaki S, Uličná L, Harata M, Hozak P. Nuclear actin filaments recruit cofilin and actin-related protein 3, and their formation is connected with a mitotic block. Histochem Cell Biol. 2014;142:139-52 pubmed publisher
	Sommer A, Hermawan A, Mickler F, Ljepoja B, Knyazev P, BrÃ¤uchle C, et al. Salinomycin co-treatment enhances tamoxifen cytotoxicity in luminal A breast tumor cells by facilitating lysosomal degradation of receptor tyrosine kinases. Oncotarget. 2016;7:50461-50476 pubmed publisher
	Bezler M, Hengstler J, Ullrich A. Inhibition of doxorubicin-induced HER3-PI3K-AKT signalling enhances apoptosis of ovarian cancer cells. Mol Oncol. 2012;6:516-29 pubmed publisher
	Liles J, Arnoletti J, Kossenkov A, Mikhaylina A, Frost A, Kulesza P, et al. Targeting ErbB3-mediated stromal-epithelial interactions in pancreatic ductal adenocarcinoma. Br J Cancer. 2011;105:523-33 pubmed publisher

product information

Catalog Number :

05-471

Subcategory :

Cell Signaling

Product Name :

Anti-erbB-3/HER-3 Antibody, clone H3.105.5

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P21860

Host Name :

Mouse

Antigen :

erbB-3/HER-3

Clone :

H3.105.5

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-erbB-3/HER-3 Antibody, clone H3.105.5

Cross Reactivity :

Human;Mouse

Background :

erbB3 (HER3, c-erbB3) is a receptor tyrosine kinase (RTK). erbB3 is one of four members of the EGFR/ErbB family of receptor tyrosine kinases. The ErbB family of receptors are a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Though erbB3 is membrane bound and can bind its ligand, it does not have an active kinase domain so it can not transmit this signal without first forming a heterodimer with other EGFR family members. This heterodimerization then leads to proliferation and differentiation. erbB3 is also known to bind Phosphoinositide 3-kinase (PI3 Kinase, PI3K) though six tyrosine phosphorylation sites that PI3K can bind to, which results in the activation of Akt/PKB. erbB3 is known to be amplified in numerous cancers including breast and prostate.

ALT Names :

Tyrosine kinase-type cell surface receptor HER3;lethal congenital contracture syndrome 2;v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3;v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)

Immunogen :

Extracellular domain of recombinant human erbB-3/HER-3 oncoprotein. Clone H3.105.5 Antibody Class IgG1, antibody produced by P3X63Ag8U1 (P3U1) x Balb/c spleen cells. Purified by ammonium sulfate precipitation, Sephacryl S-300 gel filtration and DEAE-52 chromatography.

Specificity :

This antibody recognizes erbB-3, Mr 185 kDa, but not EGF receptor, erbB-2/HER-2, or erbB-4/HER-4 Antibody Class IgG1, antibody produced by P3X63Ag8U1 (P3U1) x Balb/c spleen cells. Purified by ammonium sulfate precipitation, Sephacryl S-300 gel filtration and DEAE-52 chromatography.

Package Size :

100 µg

Uses :

Immunoprecipitation;Neutralizing

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.