GRK 2/3 (βARK 1/2) Antibody, clone C5/1.1 | EMD Millipore 05-465 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

GRK 2/3 (βARK 1/2) Antibody, clone C5/1.1

catalog :

05-465

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

C5/1.1

reactivity :

human, mouse, rat, chicken, bovine

application :

western blot, immunoprecipitation

citations: 6

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:1000; fig 2 western blot; mouse; 1:1000; fig 6	Cannavo A, Liccardo D, Eguchi A, Elliott K, Traynham C, Ibetti J, et al. Myocardial pathology induced by aldosterone is dependent on non-canonical activities of G protein-coupled receptor kinases. Nat Commun. 2016;7:10877 pubmed publisher
	Wood J, Wang J, Benovic J, Ferkey D. Structural domains required for Caenorhabditis elegans G protein-coupled receptor kinase 2 (GRK-2) function in vivo. J Biol Chem. 2012;287:12634-44 pubmed publisher
	Hennenberg M, Strittmatter F, Walther S, Hedlund P, Andersson K, Stief C, et al. ?1-adrenoceptor activation induces phosphorylation of ?2-adrenoceptors in human prostate tissue. BJU Int. 2011;108:922-8 pubmed publisher
	Bonita R, Raake P, Otis N, Chuprun J, Spivack T, Dasgupta A, et al. Dynamic changes in lymphocyte GRK2 levels in cardiac transplant patients: a biomarker for left ventricular function. Clin Transl Sci. 2010;3:14-8 pubmed publisher
	Sakamoto M, Arawaka S, Hara S, Sato H, Cui C, Machiya Y, et al. Contribution of endogenous G-protein-coupled receptor kinases to Ser129 phosphorylation of alpha-synuclein in HEK293 cells. Biochem Biophys Res Commun. 2009;384:378-82 pubmed publisher
	Maurice P, Daulat A, Broussard C, Mozo J, Clary G, Hotellier F, et al. A generic approach for the purification of signaling complexes that specifically interact with the carboxyl-terminal domain of G protein-coupled receptors. Mol Cell Proteomics. 2008;7:1556-69 pubmed publisher

product information

Catalog Number :

05-465

Subcategory :

Cell Signaling

Product Name :

Anti-GRK 2/3 (βARK 1/2) Antibody, clone C5/1.1

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P25098

Host Name :

Mouse

Antigen :

GRK 2/3 (βARK 1/2)

Clone :

C5/1.1

Conjugate :

Purified

Isotype :

IgG2aκ

Product Description :

Anti-GRK 2/3 (βARK 1/2) Antibody, clone C5/1.1

Cross Reactivity :

Human;Rat;Mouse;Bovine;Chicken;Pig;Rabbit

Background :

The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the ADRBK2 enzyme, also known as GRK3, has 85% amino acid similarity with ADRBK1, with the protein kinase catalytic domain having 95% similarity. The ADRBK2 mRNA is approximately 8 kilobases with a distribution similar to that of ADRBK1. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function. GRK2 phosphorylates the beta2 adrenergic receptor. It is a ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors.

ALT Names :

G-protein coupled receptor kinase 2;adrenergic, beta, receptor kinase 1;beta adrenergic receptor kinase 1

Immunogen :

Partial GST fusion protein corresponding to the GRK3 (βARK2) carboxyl terminus (a.a. 467-688). This immunogen shares homology with GRK2 (βARK1). The minimal epitope recognized by the antibody is a.a. 483-485. Clone C5/1.1.

Specificity :

This antibody recognizes GRK2/3, Mr 80 kDa. Additional bands at 85 and 50 kDa are also detected when using higher concentrations of the antibody.

Package Size :

100 µg

Uses :

Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.