Histone H1 Antibody, clone AE-4 | EMD Millipore 05-457 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Histone H1 Antibody, clone AE-4

catalog :

05-457

quantity :

200 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AE-4

reactivity :

human, mouse, rat, bovine, Xenopus laevis

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation

citations: 30

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; loading ...; fig 4d	de Vries J, Barendrecht A, Clark C, Urbanus R, Boross P, de Maat S, et al. Heparin Forms Polymers with Cell-free DNA Which Elongate Under Shear in Flowing Blood. Sci Rep. 2019;9:18316 pubmed publisher
western blot; human; 1:1000; fig st2	Dai Y, Hung L, Chen R, Lai C, Chang K. ON 01910.Na inhibits growth of diffuse large B-cell lymphoma by cytoplasmic sequestration of sumoylated C-MYB/TRAF6 complex. Transl Res. 2016;175:129-143.e13 pubmed publisher
western blot; human	De Zio D, Molinari F, Rizza S, Gatta L, Ciotti M, Salvatore A, et al. Apaf1-deficient cortical neurons exhibit defects in axonal outgrowth. Cell Mol Life Sci. 2015;72:4173-91 pubmed publisher
immunocytochemistry; human western blot; human	Dwivedi N, Neeli I, Schall N, Wan H, Desiderio D, Csernok E, et al. Deimination of linker histones links neutrophil extracellular trap release with autoantibodies in systemic autoimmunity. FASEB J. 2014;28:2840-51 pubmed publisher
western blot; human; 1:1000	Chang K, Chang W, Chang Y, Hung L, Lai C, Yeh Y, et al. Ran GTPase-activating protein 1 is a therapeutic target in diffuse large B-cell lymphoma. PLoS ONE. 2013;8:e79863 pubmed publisher
immunoprecipitation; human immunocytochemistry; human	Verma S, Cai Q, Kreider E, Lu J, Robertson E. Comprehensive analysis of LANA interacting proteins essential for viral genome tethering and persistence. PLoS ONE. 2013;8:e74662 pubmed publisher
western blot; human	Li Q, Jain M, Chen W, Li H. A multidimensional approach to an in-depth proteomics analysis of transcriptional regulators in neuroblastoma cells. J Neurosci Methods. 2013;216:118-27 pubmed publisher
western blot; Xenopus laevis	Lim C, Reversade B, Knowles B, Solter D. Optimal histone H3 to linker histone H1 chromatin ratio is vital for mesodermal competence in Xenopus. Development. 2013;140:853-60 pubmed publisher
western blot; human; 1:1000 western blot; mouse; 1:1000; fig 6b	Chase K, Sharma R. Nicotine induces chromatin remodelling through decreases in the methyltransferases GLP, G9a, Setdb1 and levels of H3K9me2. Int J Neuropsychopharmacol. 2013;16:1129-38 pubmed publisher
western blot; rat	Hoppe G, Rayborn M, Sears J. Diurnal rhythm of the chromatin protein Hmgb1 in rat photoreceptors is under circadian regulation. J Comp Neurol. 2007;501:219-30 pubmed
	Terme J, Millán Ariño L, Mayor R, Luque N, Izquierdo Bouldstridge A, Bustillos A, et al. Dynamics and dispensability of variant-specific histone H1 Lys-26/Ser-27 and Thr-165 post-translational modifications. FEBS Lett. 2014;588:2353-62 pubmed publisher
	Ting C, Huang H, Huang T, Wu C, Chen J. The mechanisms by which pardaxin, a natural cationic antimicrobial peptide, targets the endoplasmic reticulum and induces c-FOS. Biomaterials. 2014;35:3627-40 pubmed publisher
	Ahmed S, Prigent S. A nuclear export signal and oxidative stress regulate ShcD subcellular localisation: a potential role for ShcD in the nucleus. Cell Signal. 2014;26:32-40 pubmed publisher
	Zhang Q, Xu H, Huang M, Ma L, Huang Q, Yao Q, et al. MicroRNA-195 plays a tumor-suppressor role in human glioblastoma cells by targeting signaling pathways involved in cellular proliferation and invasion. Neuro Oncol. 2012;14:278-87 pubmed publisher
	Zhou J, Blackledge N, Farcas A, Klose R. Recognition of CpG island chromatin by KDM2A requires direct and specific interaction with linker DNA. Mol Cell Biol. 2012;32:479-89 pubmed publisher
	Jeanblanc M, Ragu S, Gey C, Contrepois K, Courbeyrette R, Thuret J, et al. Parallel pathways in RAF-induced senescence and conditions for its reversion. Oncogene. 2012;31:3072-85 pubmed publisher
	Terme J, Sesé B, Millán Ariño L, Mayor R, Izpisua Belmonte J, Barrero M, et al. Histone H1 variants are differentially expressed and incorporated into chromatin during differentiation and reprogramming to pluripotency. J Biol Chem. 2011;286:35347-57 pubmed publisher
	Chang K, Chen P, Chen Y, Chang Y, Su I. Dominant expression of survival signals of endoplasmic reticulum stress response in Hodgkin lymphoma. Cancer Sci. 2011;102:275-81 pubmed publisher
	Mishra B, von der Ohe M, Schulze C, Bian S, Makhina T, Loers G, et al. Functional role of the interaction between polysialic acid and extracellular histone H1. J Neurosci. 2010;30:12400-13 pubmed publisher
	Mihalcik S, Huddleston P, Wu X, Jelinek D. The structure of the TNFRSF13C promoter enables differential expression of BAFF-R during B cell ontogeny and terminal differentiation. J Immunol. 2010;185:1045-54 pubmed publisher
	O Brien S, Cao H, Nathans R, Ali A, Rana T. P-TEFb kinase complex phosphorylates histone H1 to regulate expression of cellular and HIV-1 genes. J Biol Chem. 2010;285:29713-20 pubmed publisher
	Katsuno M, Adachi H, Minamiyama M, Waza M, Doi H, Kondo N, et al. Disrupted transforming growth factor-beta signaling in spinal and bulbar muscular atrophy. J Neurosci. 2010;30:5702-12 pubmed publisher
	Cheng O, Ostrowski R, Liu W, Zhang J. Activation of liver X receptor reduces global ischemic brain injury by reduction of nuclear factor-kappaB. Neuroscience. 2010;166:1101-9 pubmed publisher
	Chang K, Chang Y, Jones D, Su I. Aberrant expression of cyclin a correlates with morphogenesis of reed-sternberg cells in Hodgkin lymphoma. Am J Clin Pathol. 2009;132:50-9 pubmed publisher
	Ohsawa S, Hamada S, Asou H, Kuida K, Uchiyama Y, Yoshida H, et al. Caspase-9 activation revealed by semaphorin 7A cleavage is independent of apoptosis in the aged olfactory bulb. J Neurosci. 2009;29:11385-92 pubmed publisher
	Andreeva A, Kutuzov M, Tkachuk V, Voyno Yasenetskaya T. T-cadherin is located in the nucleus and centrosomes in endothelial cells. Am J Physiol Cell Physiol. 2009;297:C1168-77 pubmed publisher
	Gavin D, Rosen C, Chase K, Grayson D, Tun N, Sharma R. Dimethylated lysine 9 of histone 3 is elevated in schizophrenia and exhibits a divergent response to histone deacetylase inhibitors in lymphocyte cultures. J Psychiatry Neurosci. 2009;34:232-7 pubmed
	Lee J, Gaetz J, Bugarija B, Fernandes C, Snyder G, Bush E, et al. Chromatin analysis of occluded genes. Hum Mol Genet. 2009;18:2567-74 pubmed publisher
	Gavin D, Kartan S, Chase K, Jayaraman S, Sharma R. Histone deacetylase inhibitors and candidate gene expression: An in vivo and in vitro approach to studying chromatin remodeling in a clinical population. J Psychiatr Res. 2009;43:870-6 pubmed publisher
	Nawroth R, van Zante A, Cervantes S, McManus M, Hebrok M, Rosen S. Extracellular sulfatases, elements of the Wnt signaling pathway, positively regulate growth and tumorigenicity of human pancreatic cancer cells. PLoS ONE. 2007;2:e392 pubmed

product information

Catalog Number :

05-457

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Histone H1 Antibody, clone AE-4

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P10412

Host Name :

Mouse

Antigen :

Histone H1

Clone :

AE-4

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-Histone H1 Antibody, clone AE-4

Cross Reactivity :

Human;Bovine;Green Plants

Background :

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

ALT Names :

H1 histone family, member 4;Histone H1b;histone 1, H1e;histone cluster 1, H1e

Immunogen :

Nuclei from myeloid leukemia biopsy cells. Clone AE-4.

Specificity :

This antibody recognizes histone H1, Mr 32-33 kDa. Doublet detected due to hyperphosphorylation of histone H1 subpopulation. Cross-reacts with other histone isoforms in the following order of signal strength: H3, H2B H4 H2A, as determined with recombinant protein.

Package Size :

200 µg

Uses :

ELISA;Immunocytochemistry;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.