NR1 Antibody, CT | EMD Millipore 05-432 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

NR1 Antibody, CT

catalog :

05-432

quantity :

200 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat, Xenopus laevis

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

citations: 28

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; Xenopus laevis; 1:1000; loading ...; fig s2c	Hammond Weinberger D, Wang Y, Glavis Bloom A, Spitzer N. Mechanism for neurotransmitter-receptor matching. Proc Natl Acad Sci U S A. 2020;117:4368-4374 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 2e	Han W, Li J, Pelkey K, Pandey S, Chen X, Wang Y, et al. Shisa7 is a GABAA receptor auxiliary subunit controlling benzodiazepine actions. Science. 2019;366:246-250 pubmed publisher
western blot; rat; 1:1000; fig 2	Forrest C, McNair K, Pisar M, Khalil O, Darlington L, Stone T. Altered hippocampal plasticity by prenatal kynurenine administration, kynurenine-3-monoxygenase (KMO) deletion or galantamine. Neuroscience. 2015;310:91-105 pubmed publisher
western blot; human; 1:1000; fig 2	Matsuno H, Ohi K, Hashimoto R, Yamamori H, Yasuda Y, Fujimoto M, et al. A naturally occurring null variant of the NMDA type glutamate receptor NR3B subunit is a risk factor of schizophrenia. PLoS ONE. 2015;10:e0116319 pubmed publisher
western blot; rat; 1:500; fig 3	Van Skike C, Diaz Granados J, Matthews D. Chronic intermittent ethanol exposure produces persistent anxiety in adolescent and adult rats. Alcohol Clin Exp Res. 2015;39:262-71 pubmed publisher
western blot; mouse; 1:1000; fig s5	Gascon E, Lynch K, Ruan H, Almeida S, Verheyden J, Seeley W, et al. Alterations in microRNA-124 and AMPA receptors contribute to social behavioral deficits in frontotemporal dementia. Nat Med. 2014;20:1444-51 pubmed publisher
immunoprecipitation; mouse; 10 ug western blot; mouse	Grau C, AratÃ³ K, FernÃ¡ndez FernÃ¡ndez J, Valderrama A, Sindreu C, Fillat C, et al. DYRK1A-mediated phosphorylation of GluN2A at Ser(1048) regulates the surface expression and channel activity of GluN1/GluN2A receptors. Front Cell Neurosci. 2014;8:331 pubmed publisher
western blot; mouse	Trotter J, Lee G, Kazdoba T, Crowell B, Domogauer J, Mahoney H, et al. Dab1 is required for synaptic plasticity and associative learning. J Neurosci. 2013;33:15652-68 pubmed publisher
western blot; rat; 1:500; fig 1	Wei J, Yuen E, Liu W, Li X, Zhong P, Karatsoreos I, et al. Estrogen protects against the detrimental effects of repeated stress on glutamatergic transmission and cognition. Mol Psychiatry. 2014;19:588-98 pubmed publisher
western blot; rat; 1:1000	Gupta S, Hillman B, Prakash A, Ugale R, Stairs D, Dravid S. Effect of D-cycloserine in conjunction with fear extinction training on extracellular signal-regulated kinase activation in the medial prefrontal cortex and amygdala in rat. Eur J Neurosci. 2013;37:1811-22 pubmed publisher
immunohistochemistry; mouse western blot; mouse; 1:500	Kopeikina K, Polydoro M, Tai H, Yaeger E, Carlson G, Pitstick R, et al. Synaptic alterations in the rTg4510 mouse model of tauopathy. J Comp Neurol. 2013;521:1334-53 pubmed publisher
	Morel C, Sherrin T, Kennedy N, Forest K, Avcioglu Barutcu S, Robles M, et al. JIP1-Mediated JNK Activation Negatively Regulates Synaptic Plasticity and Spatial Memory. J Neurosci. 2018;38:3708-3728 pubmed publisher
	Pressey J, Mahadevan V, Khademullah C, Dargaei Z, Chevrier J, Ye W, et al. A kainate receptor subunit promotes the recycling of the neuron-specific K+-Cl- co-transporter KCC2 in hippocampal neurons. J Biol Chem. 2017;292:6190-6201 pubmed publisher
	Garcia Alvarez G, Lu B, Yap K, Wong L, Thevathasan J, Lim L, et al. STIM2 regulates PKA-dependent phosphorylation and trafficking of AMPARs. Mol Biol Cell. 2015;26:1141-59 pubmed publisher
	Parent M, Hottman D, Cheng S, Zhang W, McMahon L, Yuan L, et al. Simvastatin treatment enhances NMDAR-mediated synaptic transmission by upregulating the surface distribution of the GluN2B subunit. Cell Mol Neurobiol. 2014;34:693-705 pubmed publisher
	Dong L, Chen J, Li F, Gao F, Wu J, Miao Y, et al. Enhanced expression of NR2B subunits of NMDA receptors in the inherited glaucomatous DBA/2J mouse retina. Neural Plast. 2013;2013:670254 pubmed publisher
	Mielke J. Susceptibility to oxygen-glucose deprivation is reduced in acute hippocampal slices from euthermic Syrian golden hamsters relative to slices from Sprague-Dawley rats. Neurosci Lett. 2013;553:13-7 pubmed publisher
	Itakura M, Tsujimura J, Yamamori S, Ohkido T, Takahashi M. NMDA receptor-dependent recruitment of calnexin to the neuronal plasma membrane. Neurosci Lett. 2013;550:173-8 pubmed publisher
	Ibrahim F, Maragkakis M, Alexiou P, Maronski M, Dichter M, Mourelatos Z. Identification of in vivo, conserved, TAF15 RNA binding sites reveals the impact of TAF15 on the neuronal transcriptome. Cell Rep. 2013;3:301-8 pubmed publisher
	Bayes A, Collins M, Croning M, van de Lagemaat L, Choudhary J, Grant S. Comparative study of human and mouse postsynaptic proteomes finds high compositional conservation and abundance differences for key synaptic proteins. PLoS ONE. 2012;7:e46683 pubmed publisher
	Gleichman A, Spruce L, Dalmau J, Seeholzer S, Lynch D. Anti-NMDA receptor encephalitis antibody binding is dependent on amino acid identity of a small region within the GluN1 amino terminal domain. J Neurosci. 2012;32:11082-94 pubmed publisher
	She K, Ferreira J, Carvalho A, Craig A. Glutamate binding to the GluN2B subunit controls surface trafficking of N-methyl-D-aspartate (NMDA) receptors. J Biol Chem. 2012;287:27432-45 pubmed publisher
	Forrest C, Khalil O, Pisar M, Smith R, Darlington L, Stone T. Prenatal activation of Toll-like receptors-3 by administration of the viral mimetic poly(I:C) changes synaptic proteins, N-methyl-D-aspartate receptors and neurogenesis markers in offspring. Mol Brain. 2012;5:22 pubmed publisher
	Pitchers K, Schmid S, Di Sebastiano A, Wang X, Laviolette S, Lehman M, et al. Natural reward experience alters AMPA and NMDA receptor distribution and function in the nucleus accumbens. PLoS ONE. 2012;7:e34700 pubmed publisher
	She K, Craig A. NMDA receptors mediate synaptic competition in culture. PLoS ONE. 2011;6:e24423 pubmed publisher
	Francis K, Wei L. Human embryonic stem cell neural differentiation and enhanced cell survival promoted by hypoxic preconditioning. Cell Death Dis. 2010;1:e22 pubmed publisher
	Ghasemzadeh M, Vasudevan P, Mueller C. Locomotor sensitization to cocaine is associated with distinct pattern of glutamate receptor trafficking to the postsynaptic density in prefrontal cortex: early versus late withdrawal effects. Pharmacol Biochem Behav. 2009;92:383-92 pubmed publisher
	Ghasemzadeh M, Mueller C, Vasudevan P. Behavioral sensitization to cocaine is associated with increased glutamate receptor trafficking to the postsynaptic density after extended withdrawal period. Neuroscience. 2009;159:414-26 pubmed publisher

product information

Catalog Number :

05-432

Subcategory :

Neuroscience

Product Name :

Anti-NR1 Antibody, CT

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P35439

Host Name :

Mouse

Antigen :

NR1

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-NR1 Antibody, CT

Cross Reactivity :

Human;Rat;Mouse

Background :

NR1 (NMDA1or NMDAR1, also known as GRIN1) along with the NR2 subunit forms a heterodimer that make up the NMDA receptor. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteriztic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.

ALT Names :

Grin1;NMDA R1 receptor C1 cassette ;glutamate receptor, ionotropic, N-methyl D-aspartate 1

Immunogen :

His-tag fusion protein corresponding to residues 834-938 of rat NR1A. The immunizing sequence is identical in rat and human.

Specificity :

This antibody is specific for NR1, Mr 130 kDa. The epitope maps to a region (834-864) common to all NR1 isoforms and so should cross react with all NR1 isoforms. An additional band may be detected at higher concentrations, Mr 200kDa.

Package Size :

200 µg

Uses :

Immunocytochemistry;Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at 2° to 8ºC from date of receipt.