PP2A Antibody, C subunit, clone 1D6 | EMD Millipore 05-421 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PP2A Antibody, C subunit, clone 1D6

catalog :

05-421

quantity :

200 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1D6

The same clone is also sold as:

Santa Cruz Biotechnology: sc-80665

reactivity :

green monkey, human, mouse, rat, dogs, bovine, Xenopus laevis

application :

western blot, immunocytochemistry, immunoprecipitation

citations: 43

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:2000; loading ...; fig 4a	Wang X, Garvanska D, Nasa I, Ueki Y, Zhang G, Kettenbach A, et al. A dynamic charge-charge interaction modulates PP2A:B56 substrate recruitment. elife. 2020;9: pubmed publisher
western blot; human; loading ...; fig 2d	Hein J, Hertz E, Garvanska D, Kruse T, Nilsson J. Distinct kinetics of serine and threonine dephosphorylation are essential for mitosis. Nat Cell Biol. 2017;19:1433-1440 pubmed publisher
western blot; mouse; fig s3	Zhang T, Du W, Wilson A, Namekawa S, Andreassen P, Meetei A, et al. Fancd2 in vivo interaction network reveals a non-canonical role in mitochondrial function. Sci Rep. 2017;7:45626 pubmed publisher
immunoprecipitation; human western blot; human; loading ...; fig 2l	Fan L, Liu M, Guo M, Hu C, Yan Z, Chen J, et al. FAM122A, a new endogenous inhibitor of protein phosphatase 2A. Oncotarget. 2016;7:63887-63900 pubmed publisher
immunoprecipitation; rat; loading ...; fig 2a immunocytochemistry; rat; 1:200; loading ...; fig 4a western blot; rat; 1:2000; loading ...; fig 2a	Juraszek B, Nałęcz K. Protein phosphatase PP2A - a novel interacting partner of carnitine transporter OCTN2 (SLC22A5) in rat astrocytes. J Neurochem. 2016;139:537-551 pubmed publisher
immunoprecipitation; human; fig 4 western blot; human; fig 4	Jeong A, Han S, Lee S, Su Park J, Lu Y, Yu S, et al. Patient derived mutation W257G of PPP2R1A enhances cancer cell migration through SRC-JNK-c-Jun pathway. Sci Rep. 2016;6:27391 pubmed publisher
western blot; rat; 1:2000; fig s1	Sadredini M, Danielsen T, Aronsen J, Manotheepan R, Hougen K, Sjaastad I, et al. Beta-Adrenoceptor Stimulation Reveals Ca2+ Waves and Sarcoplasmic Reticulum Ca2+ Depletion in Left Ventricular Cardiomyocytes from Post-Infarction Rats with and without Heart Failure. PLoS ONE. 2016;11:e0153887 pubmed publisher
immunoprecipitation; human; fig s3 western blot; human; fig s3 western blot; mouse; fig 3	Apostolidis S, RodrÃguez RodrÃguez N, SuÃ¡rez Fueyo A, Dioufa N, Ozcan E, CrispÃn J, et al. Phosphatase PP2A is requisite for the function of regulatory T cells. Nat Immunol. 2016;17:556-64 pubmed publisher
western blot; human; 1:2500; fig 2	Hein J, Nilsson J. Interphase APC/C-Cdc20 inhibition by cyclin A2-Cdk2 ensures efficient mitotic entry. Nat Commun. 2016;7:10975 pubmed publisher
western blot; human; fig 5	Lee S, Jeong A, Park J, Han S, Jang C, Kim K, et al. IK-guided PP2A suppresses Aurora B activity in the interphase of tumor cells. Cell Mol Life Sci. 2016;73:3375-86 pubmed publisher
immunoprecipitation; human; fig 2 western blot; human; fig 2	Katoh I, Fukunishi N, Fujimuro M, Kasai H, Moriishi K, Hata R, et al. Repression of Wnt/Î²-catenin response elements by p63 (TP63). Cell Cycle. 2016;15:699-710 pubmed publisher
western blot; human; fig 6	Asano Y, Kawase T, Okabe A, Tsutsumi S, Ichikawa H, Tatebe S, et al. IER5 generates a novel hypo-phosphorylated active form of HSF1 and contributes to tumorigenesis. Sci Rep. 2016;6:19174 pubmed publisher
immunoprecipitation; mouse; fig s6	Yabe R, Miura A, Usui T, Mudrak I, Ogris E, Ohama T, et al. Protein Phosphatase Methyl-Esterase PME-1 Protects Protein Phosphatase 2A from Ubiquitin/Proteasome Degradation. PLoS ONE. 2015;10:e0145226 pubmed publisher
immunoprecipitation; mouse; fig s9	Hu X, Garcia C, Fazli L, Gleave M, Vitek M, Jansen M, et al. Inhibition of Pten deficient Castration Resistant Prostate Cancer by Targeting of the SET - PP2A Signaling axis. Sci Rep. 2015;5:15182 pubmed publisher
western blot; human	Voets E, Wolthuis R. MASTL promotes cyclin B1 destruction by enforcing Cdc20-independent binding of cyclin B1 to the APC/C. Biol Open. 2015;4:484-95 pubmed publisher
immunoprecipitation; human; fig 1 western blot; human; fig 2	Kwun H, Shuda M, Camacho C, Gamper A, Thant M, Chang Y, et al. Restricted protein phosphatase 2A targeting by Merkel cell polyomavirus small T antigen. J Virol. 2015;89:4191-200 pubmed publisher
western blot; mouse; fig 3	Sontag J, Wasek B, Taleski G, Smith J, Arning E, Sontag E, et al. Altered protein phosphatase 2A methylation and Tau phosphorylation in the young and aged brain of methylenetetrahydrofolate reductase (MTHFR) deficient mice. Front Aging Neurosci. 2014;6:214 pubmed publisher
western blot; mouse; 1:2000	Chasseigneaux S, Clamagirand C, Huguet L, Gorisse Hussonnois L, Rose C, Allinquant B. Cytoplasmic SET induces tau hyperphosphorylation through a decrease of methylated phosphatase 2A. BMC Neurosci. 2014;15:82 pubmed publisher
immunoprecipitation; mouse; 4 ug	Mamouni K, Cristini A, Guirouilh Barbat J, Monferran S, Lemarie A, Faye J, et al. RhoB promotes ?H2AX dephosphorylation and DNA double-strand break repair. Mol Cell Biol. 2014;34:3144-55 pubmed publisher
western blot; Xenopus laevis; 0.2 ug/ml	Kang Q, Srividhya J, Ipe J, Pomerening J. Evidence toward a dual phosphatase mechanism that restricts Aurora A (Thr-295) phosphorylation during the early embryonic cell cycle. J Biol Chem. 2014;289:17480-96 pubmed publisher
western blot; human	Dou X, Xia Y, Chen J, Qian Y, Li S, Zhang X, et al. Rectification of impaired adipose tissue methylation status and lipolytic response contributes to hepatoprotective effect of betaine in a mouse model of alcoholic liver disease. Br J Pharmacol. 2014;171:4073-86 pubmed publisher
western blot; green monkey; 1:1000	Jamin A, Wicklund A, Wiebe M. Cell- and virus-mediated regulation of the barrier-to-autointegration factor's phosphorylation state controls its DNA binding, dimerization, subcellular localization, and antipoxviral activity. J Virol. 2014;88:5342-55 pubmed publisher
western blot; mouse; 1:800	Perera N, Sheean R, Scott J, Kemp B, Horne M, Turner B. Mutant TDP-43 deregulates AMPK activation by PP2A in ALS models. PLoS ONE. 2014;9:e90449 pubmed publisher
immunoprecipitation; rat western blot; rat	Longman M, Ranieri A, Avkiran M, Snabaitis A. Regulation of PP2AC carboxylmethylation and cellular localisation by inhibitory class G-protein coupled receptors in cardiomyocytes. PLoS ONE. 2014;9:e86234 pubmed publisher
western blot; mouse; 1:500	Honarpour N, Rose C, Brumbaugh J, Anderson J, Graham R, Sweredoski M, et al. F-box protein FBXL16 binds PP2A-B55? and regulates differentiation of embryonic stem cells along the FLK1+ lineage. Mol Cell Proteomics. 2014;13:780-91 pubmed publisher
immunoprecipitation; human western blot; human	Sreevalsan S, Safe S. The cannabinoid WIN 55,212-2 decreases specificity protein transcription factors and the oncogenic cap protein eIF4E in colon cancer cells. Mol Cancer Ther. 2013;12:2483-93 pubmed publisher
western blot; human	Arampatzi P, Gialitakis M, Makatounakis T, Papamatheakis J. Gene-specific factors determine mitotic expression and bookmarking via alternate regulatory elements. Nucleic Acids Res. 2013;41:2202-15 pubmed publisher
western blot; human; 1:500 western blot; dogs; 1:500 western blot; rat; 1:500 immunocytochemistry; mouse western blot; mouse; 1:500	Degrande S, Little S, Nixon D, Wright P, Snyder J, Dun W, et al. Molecular mechanisms underlying cardiac protein phosphatase 2A regulation in heart. J Biol Chem. 2013;288:1032-46 pubmed publisher
	Wu C, Zheng A, Jiang L, Rowse M, Stanevich V, Chen H, et al. Methylation-regulated decommissioning of multimeric PP2A complexes. Nat Commun. 2017;8:2272 pubmed publisher
	Aldiri I, Ajioka I, Xu B, Zhang J, Chen X, Benavente C, et al. Brg1 coordinates multiple processes during retinogenesis and is a tumor suppressor in retinoblastoma. Development. 2015;142:4092-106 pubmed publisher
	Wong P, Feng Y, Wang J, Shi R, Jiang X. Regulation of autophagy by coordinated action of mTORC1 and protein phosphatase 2A. Nat Commun. 2015;6:8048 pubmed publisher
	Cianfanelli V, Fuoco C, Lorente M, Salazar M, Quondamatteo F, Gherardini P, et al. AMBRA1 links autophagy to cell proliferation and tumorigenesis by promoting c-Myc dephosphorylation and degradation. Nat Cell Biol. 2015;17:20-30 pubmed publisher
	Williams B, Filter J, Blake Hodek K, Wadzinski B, Fuda N, Shalloway D, et al. Greatwall-phosphorylated Endosulfine is both an inhibitor and a substrate of PP2A-B55 heterotrimers. elife. 2014;3:e01695 pubmed publisher
	Farrell K, Krishnamachari S, Villanueva E, Lou H, Alerte T, Peet E, et al. Non-motor parkinsonian pathology in aging A53T ?-synuclein mice is associated with progressive synucleinopathy and altered enzymatic function. J Neurochem. 2014;128:536-46 pubmed publisher
	Chuang M, Wu S, Tang S, Lai X, Lai H, Hsu K, et al. The HDAC inhibitor LBH589 induces ERK-dependent prometaphase arrest in prostate cancer via HDAC6 inactivation and down-regulation. PLoS ONE. 2013;8:e73401 pubmed publisher
	Mackay K, Tu Y, Young S, Clarke S. Circumventing embryonic lethality with Lcmt1 deficiency: generation of hypomorphic Lcmt1 mice with reduced protein phosphatase 2A methyltransferase expression and defects in insulin signaling. PLoS ONE. 2013;8:e65967 pubmed publisher
	Wu J, Lou H, Alerte T, Stachowski E, Chen J, Singleton A, et al. Lewy-like aggregation of Î±-synuclein reduces protein phosphatase 2A activity in vitro and in vivo. Neuroscience. 2012;207:288-97 pubmed publisher
	Wang Y, Steimle P, Ren Y, Ross C, Robinson D, Egelhoff T, et al. Dictyostelium huntingtin controls chemotaxis and cytokinesis through the regulation of myosin II phosphorylation. Mol Biol Cell. 2011;22:2270-81 pubmed publisher
	Münz T, Litterst C, Pfitzner E. Interaction of STAT6 with its co-activator SRC-1/NCoA-1 is regulated by dephosphorylation of the latter via PP2A. Nucleic Acids Res. 2011;39:3255-66 pubmed publisher
	Xu H, Ginsburg K, Hall D, Zimmermann M, Stein I, Zhang M, et al. Targeting of protein phosphatases PP2A and PP2B to the C-terminus of the L-type calcium channel Ca v1.2. Biochemistry. 2010;49:10298-307 pubmed publisher
	Rahman A, Brew B, Guillemin G. Lead dysregulates serine/threonine protein phosphatases in human neurons. Neurochem Res. 2011;36:195-204 pubmed publisher
	Lou H, Montoya S, Alerte T, Wang J, Wu J, Peng X, et al. Serine 129 phosphorylation reduces the ability of alpha-synuclein to regulate tyrosine hydroxylase and protein phosphatase 2A in vitro and in vivo. J Biol Chem. 2010;285:17648-61 pubmed publisher
	Trotta R, Ciarlariello D, Dal Col J, Allard J, Neviani P, Santhanam R, et al. The PP2A inhibitor SET regulates natural killer cell IFN-gamma production. J Exp Med. 2007;204:2397-405 pubmed

product information

Catalog Number :

05-421

Subcategory :

Cell Signaling

Product Name :

Anti-PP2A Antibody, C subunit, clone 1D6

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P67775

Host Name :

Mouse

Antigen :

PP2A

Clone :

1D6

Conjugate :

Purified

Isotype :

IgG2bκ

Product Description :

Anti-PP2A Antibody, C subunit, clone 1D6

Cross Reactivity :

Human;Rat;Mouse;Bovine;Rabbit;Xenopus;Yeast ( S. cerevisiae )

Background :

Type 2 protein serine/threonine phosphatase (PP2A) is a trimer composed of a catalytic C subunit and two regulatory (A and B) subunits; many isoforms for the A and B subunits have been found. PP2A has a high specific activity against the α subunit of phosphorylase kinase and is insensitive to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and by its high sensitivity to inhibition by okadaic acid. It is also thought to be the principal downmodulator of serine/threonine kinase cascades. PP2B requires Ca2+/calmodulin for activity, and is an activator of NFAT transcription factors. PP2C requires Mg2+, but is insensitive to okadaic acid, microcystin LR, or the calmodulin inhibitor trifluoperazine.

ALT Names :

Replication protein C;protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform;protein phosphatase 2, catalytic subunit, alpha isoform;serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform

Immunogen :

A 16 residue synthetic peptide (C-RGEPHVTRRTPDYFL) corresponding to amino acids 295-309 of the 36 kDa catalytic subunit of human protein phosphatase 2A (PP2A). Clone 1D6.

Specificity :

This antibody recognizes PP2A catalytic subunit, Mr 36 kDa.

Package Size :

200 µg

Uses :

Immunocytochemistry;Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.