Paxillin Antibody, clone 5H11 | EMD Millipore 05-417 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Paxillin Antibody, clone 5H11

catalog :

05-417

quantity :

250 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5H11

The same clone is also sold as:

LifeSpan Biosciences: LS-C88170, LS-B8618
Invitrogen: MA5-13356, AHO0492

reactivity :

guinea pig, human, mouse, rat, chicken, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

citations: 39

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; loading ...; fig s4b	Owen L, Adhikari A, Patel M, Grimmer P, Leijnse N, Kim M, et al. A cytoskeletal clutch mediates cellular force transmission in a soft, three-dimensional extracellular matrix. Mol Biol Cell. 2017;28:1959-1974 pubmed publisher
immunohistochemistry; human; loading ...; fig 8d western blot; human; loading ...; fig s8a	Mekhdjian A, Kai F, Rubashkin M, Prahl L, Przybyla L, McGregor A, et al. Integrin-mediated traction force enhances paxillin molecular associations and adhesion dynamics that increase the invasiveness of tumor cells into a three-dimensional extracellular matrix. Mol Biol Cell. 2017;28:1467-1488 pubmed publisher
western blot; human; 1:1000; loading ...; fig 1b, 1d	Yoo S, Latifkar A, Cerione R, Antonyak M. Cool-associated Tyrosine-phosphorylated Protein 1 Is Required for the Anchorage-independent Growth of Cervical Carcinoma Cells by Binding Paxillin and Promoting AKT Activation. J Biol Chem. 2017;292:3947-3957 pubmed publisher
immunocytochemistry; mouse; loading ...; fig 8	Liu R, Jin J. Deletion of calponin 2 in macrophages alters cytoskeleton-based functions and attenuates the development of atherosclerosis. J Mol Cell Cardiol. 2016;99:87-99 pubmed publisher
western blot; human; 1:1000; loading ...	Harrington K, Clevenger C. Identification of NEK3 Kinase Threonine 165 as a Novel Regulatory Phosphorylation Site That Modulates Focal Adhesion Remodeling Necessary for Breast Cancer Cell Migration. J Biol Chem. 2016;291:21388-21406 pubmed
western blot; guinea pig; 1:500; fig 7	Ãlvarez Santos M, Carbajal V, Tellez JimÃ©nez O, MartÃnez Cordero E, Ruiz V, HernÃ¡ndez Pando R, et al. Airway Hyperresponsiveness in Asthma Model Occurs Independently of Secretion of Î²1 Integrins in Airway Wall and Focal Adhesions Proteins Down Regulation. J Cell Biochem. 2016;117:2385-96 pubmed publisher
immunocytochemistry; mouse; 1:100; loading ...; fig 6c	Bulla R, Tripodo C, Rami D, Ling G, Agostinis C, Guarnotta C, et al. C1q acts in the tumour microenvironment as a cancer-promoting factor independently of complement activation. Nat Commun. 2016;7:10346 pubmed publisher
immunocytochemistry; human; loading ...; fig 1h	Awad K, Elinoff J, Wang S, Gairhe S, Ferreyra G, Cai R, et al. Raf/ERK drives the proliferative and invasive phenotype of BMPR2-silenced pulmonary artery endothelial cells. Am J Physiol Lung Cell Mol Physiol. 2016;310:L187-201 pubmed publisher
immunocytochemistry; mouse; 1:200; fig 3 western blot; rat; 1:1000; fig 2	Fiore V, Strane P, Bryksin A, White E, Hagood J, Barker T. Conformational coupling of integrin and Thy-1 regulates Fyn priming and fibroblast mechanotransduction. J Cell Biol. 2015;211:173-90 pubmed publisher
immunocytochemistry; mouse; fig 2	Gopal S, SÃ¸gaard P, Multhaupt H, Pataki C, Okina E, Xian X, et al. Transmembrane proteoglycans control stretch-activated channels to set cytosolic calcium levels. J Cell Biol. 2015;210:1199-211 pubmed publisher
immunocytochemistry; human; 1:100; fig 8	Lim H, Multhaupt H, Couchman J. Cell surface heparan sulfate proteoglycans control adhesion and invasion of breast carcinoma cells. Mol Cancer. 2015;14:15 pubmed publisher
immunocytochemistry; mouse	Hoon J, Li H, Koh C. POPX2 phosphatase regulates cell polarity and centrosome placement. Cell Cycle. 2014;13:2459-68 pubmed publisher
immunohistochemistry; human; 1:500; loading ...; fig 1b	Majeed S, Vasudevan L, Chen C, Luo Y, Torres J, Evans T, et al. Clathrin light chains are required for the gyrating-clathrin recycling pathway and thereby promote cell migration. Nat Commun. 2014;5:3891 pubmed publisher
western blot; human	Chen Q, Xu R, Zeng C, Lu Q, Huang D, Shi C, et al. Down-regulation of Gli transcription factor leads to the inhibition of migration and invasion of ovarian cancer cells via integrin ?4-mediated FAK signaling. PLoS ONE. 2014;9:e88386 pubmed publisher
immunocytochemistry; mouse; 5-10 mg/ml	Wu T, Kooi C, Shah P, Charnigo R, Huang C, Smyth S, et al. Integrin-mediated cell surface recruitment of autotaxin promotes persistent directional cell migration. FASEB J. 2014;28:861-70 pubmed publisher
immunocytochemistry; human	Au S, Wong C, Lee J, Wong C, Ng I. EZH2-Mediated H3K27me3 Is Involved in Epigenetic Repression of Deleted in Liver Cancer 1 in Human Cancers. PLoS ONE. 2013;8:e68226 pubmed publisher
western blot; human	Doddrell R, Dun X, Shivane A, Feltri M, Wrabetz L, Wegner M, et al. Loss of SOX10 function contributes to the phenotype of human Merlin-null schwannoma cells. Brain. 2013;136:549-63 pubmed publisher
	Caino M, Seo J, Wang Y, Rivadeneira D, Gabrilovich D, Kim E, et al. Syntaphilin controls a mitochondrial rheostat for proliferation-motility decisions in cancer. J Clin Invest. 2017;127:3755-3769 pubmed publisher
	Zhang L, Luga V, Armitage S, Musiol M, Won A, Yip C, et al. A lateral signalling pathway coordinates shape volatility during cell migration. Nat Commun. 2016;7:11714 pubmed publisher
	Wang Y, Yan F, Ye Q, Wu X, Jiang F. PTP1B inhibitor promotes endothelial cell motility by activating the DOCK180/Rac1 pathway. Sci Rep. 2016;6:24111 pubmed publisher
	Lim H, Couchman J. Syndecan-2 regulation of morphology in breast carcinoma cells is dependent on RhoGTPases. Biochim Biophys Acta. 2014;1840:2482-90 pubmed publisher
	Bijian K, Lougheed C, Su J, Xu B, Yu H, Wu J, et al. Targeting focal adhesion turnover in invasive breast cancer cells by the purine derivative reversine. Br J Cancer. 2013;109:2810-8 pubmed publisher
	Ho C, Jaalouk D, Vartiainen M, Lammerding J. Lamin A/C and emerin regulate MKL1-SRF activity by modulating actin dynamics. Nature. 2013;497:507-11 pubmed publisher
	Ovcharenko A, Granot G, Shpilberg O, Raanani P. Retinoic acid induces adhesion and migration in NB4 cells through Pyk2 signaling. Leuk Res. 2013;37:956-62 pubmed publisher
	Beaumont K, Mrksich M. The mechanostability of isolated focal adhesions is strongly dependent on pH. Chem Biol. 2012;19:711-20 pubmed publisher
	Mohammad I, Arora P, Naghibzadeh Y, Wang Y, Li J, Mascarenhas W, et al. Flightless I is a focal adhesion-associated actin-capping protein that regulates cell migration. FASEB J. 2012;26:3260-72 pubmed publisher
	Ferrando I, Chaerkady R, Zhong J, Molina H, Jacob H, Herbst Robinson K, et al. Identification of targets of c-Src tyrosine kinase by chemical complementation and phosphoproteomics. Mol Cell Proteomics. 2012;11:355-69 pubmed publisher
	Karavitis J, Murdoch E, Deburghgraeve C, Ramirez L, Kovacs E. Ethanol suppresses phagosomal adhesion maturation, Rac activation, and subsequent actin polymerization during Fc?R-mediated phagocytosis. Cell Immunol. 2012;274:61-71 pubmed publisher
	Min J, Reznichenko M, Poythress R, Gallant C, Vetterkind S, Li Y, et al. Src modulates contractile vascular smooth muscle function via regulation of focal adhesions. J Cell Physiol. 2012;227:3585-92 pubmed publisher
	Huang C, Fu X, Liu J, Qi Y, Li S, Wang H. The involvement of integrin ?1 signaling in the migration and myofibroblastic differentiation of skin fibroblasts on anisotropic collagen-containing nanofibers. Biomaterials. 2012;33:1791-800 pubmed publisher
	Wu Z, Li X, Sunkara M, Spearman H, Morris A, Huang C. PIPKI? regulates focal adhesion dynamics and colon cancer cell invasion. PLoS ONE. 2011;6:e24775 pubmed publisher
	Sánchez Cortés J, Mrksich M. Using self-assembled monolayers to understand ?8?1-mediated cell adhesion to RGD and FEI motifs in nephronectin. ACS Chem Biol. 2011;6:1078-86 pubmed publisher
	March M, Long E. Î²2 integrin induces TCRÎ¶-Syk-phospholipase C-Î³ phosphorylation and paxillin-dependent granule polarization in human NK cells. J Immunol. 2011;186:2998-3005 pubmed publisher
	Lapierre L, Caldwell C, Higginbotham J, Avant K, Hall J, Beauchamp R, et al. Transformation of rat intestinal epithelial cells by overexpression of Rab25 is microtubule dependent. Cytoskeleton (Hoboken). 2011;68:97-111 pubmed publisher
	Kuzelova K, Pluskalova M, Grebeňová D, Pavlaskova K, Halada P, Hrkal Z. Changes in cell adhesivity and cytoskeleton-related proteins during imatinib-induced apoptosis of leukemic JURL-MK1 cells. J Cell Biochem. 2010;111:1413-25 pubmed publisher
	Miranda L, Carpentier S, Platek A, Hussain N, Gueuning M, Vertommen D, et al. AMP-activated protein kinase induces actin cytoskeleton reorganization in epithelial cells. Biochem Biophys Res Commun. 2010;396:656-61 pubmed publisher
	Jiao X, Katiyar S, Willmarth N, Liu M, Ma X, Flomenberg N, et al. c-Jun induces mammary epithelial cellular invasion and breast cancer stem cell expansion. J Biol Chem. 2010;285:8218-26 pubmed publisher
	Kuzelova K, Pluskalová M, Brodska B, Otevrelova P, Elknerová K, Grebenová D, et al. Suberoylanilide hydroxamic acid (SAHA) at subtoxic concentrations increases the adhesivity of human leukemic cells to fibronectin. J Cell Biochem. 2010;109:184-95 pubmed publisher
	Kreis P, Rousseau V, Thévenot E, Combeau G, Barnier J. The four mammalian splice variants encoded by the p21-activated kinase 3 gene have different biological properties. J Neurochem. 2008;106:1184-97 pubmed publisher

product information

Catalog Number :

05-417

Subcategory :

Cell Signaling

Product Name :

Anti-Paxillin Antibody, clone 5H11

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P49023

Host Name :

Mouse

Antigen :

Paxillin

Clone :

5H11

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Paxillin Antibody, clone 5H11

Cross Reactivity :

Human;Rat;Mouse;Bovine;Avian

Background :

Paxillin, a focal adhesion scaffolding protein, is phosphorylated on multiple tyrosine residues in response various stimulations including integrin stimulation, growth factor stimulation, and oncogenic transformation (v-Src, v-Crk and BCR/ABL). Paxillin binds many other focal adhesion proteins including FAK, Vinculin, Talin, and Tensin. As such, Paxillin and it tyrosine phosphorylation, help regulate the dynamics of the focal adhesions and the actin cytoskeleton which results in cell morphology and cell migration characteristics of the cell. Paxillin is most notable phosphorylated on tyrosines 31 and 118 (Tyr31 and Tyr118). Interestingly, Vinculin and FAK bind to overlapping sites on Paxillin. Additionally, there is an increase in Paxillin phosphorylation at Tyr118 in vinculin (-/-) and Vinculin Y822F cells. Paxillin Y31F Y118F expression restores apoptosis and inhibits Erk activity.

ALT Names :

FLJ16691;paxillin

Immunogen :

GST fusion protein containing fulllength human paxillin. Clone 5H11.

Specificity :

This antibody recognizes paxillin.

Package Size :

250 µg

Uses :

Immunocytochemistry;Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.