phospho-GSK3 (Tyr279/Tyr216) Antibody, clone 5G-2F | EMD Millipore 05-413 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-GSK3 (Tyr279/Tyr216) Antibody, clone 5G-2F

catalog :

05-413

quantity :

200 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

5G-2F

reactivity :

human, mouse, rat

application :

western blot

citations: 13

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:250; loading ...; fig 1e	Sarikhani M, Mishra S, Maity S, Kotyada C, Wolfgeher D, Gupta M, et al. SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation. elife. 2018;7: pubmed publisher
western blot; human; loading ...; fig 7b western blot; rat	Nasri I, Bonnet D, Zwarycz B, d Aldebert E, Khou S, Mezghani Jarraya R, et al. PAR2-dependent activation of GSK3? regulates the survival of colon stem/progenitor cells. Am J Physiol Gastrointest Liver Physiol. 2016;311:G221-36 pubmed publisher
western blot; rat; 1:1000; fig 2	Garcia RatÃ©s S, Morrill P, Tu H, Pottiez G, Badin A, Tormo Garcia C, et al. (I) Pharmacological profiling of a novel modulator of the Î±7 nicotinic receptor: Blockade of a toxic acetylcholinesterase-derived peptide increased in Alzheimer brains. Neuropharmacology. 2016;105:487-499 pubmed publisher
western blot; mouse; 1:2000	Radford H, Moreno J, Verity N, Halliday M, Mallucci G. PERK inhibition prevents tau-mediated neurodegeneration in a mouse model of frontotemporal dementia. Acta Neuropathol. 2015;130:633-42 pubmed publisher
western blot; rat; 1:5000; fig 5d	Ohnishi T, Yanazawa M, Sasahara T, Kitamura Y, Hiroaki H, Fukazawa Y, et al. Na, K-ATPase α3 is a death target of Alzheimer patient amyloid-β assembly. Proc Natl Acad Sci U S A. 2015;112:E4465-74 pubmed publisher
western blot; human; tbl s3	Cheng H, Liang Y, Kuo Y, Chuu C, Lin C, Lee M, et al. Identification of thioridazine, an antipsychotic drug, as an antiglioblastoma and anticancer stem cell agent using public gene expression data. Cell Death Dis. 2015;6:e1753 pubmed publisher
western blot; human; 1:1000; fig 5f	Riise J, Plath N, Pakkenberg B, Parachikova A. Aberrant Wnt signaling pathway in medial temporal lobe structures of Alzheimer's disease. J Neural Transm (Vienna). 2015;122:1303-18 pubmed publisher
	Musazzi L, Seguini M, Mallei A, Treccani G, Pelizzari M, Tornese P, et al. Time-dependent activation of MAPK/Erk1/2 and Akt/GSK3 cascades: modulation by agomelatine. BMC Neurosci. 2014;15:119 pubmed publisher
	Laurent C, Eddarkaoui S, Derisbourg M, Leboucher A, Demeyer D, Carrier S, et al. Beneficial effects of caffeine in a transgenic model of Alzheimer's disease-like tau pathology. Neurobiol Aging. 2014;35:2079-90 pubmed publisher
	Neumann P, Alsaffar H, Gertzberg N, Johnson A. Inhibition of GSK3?/? promotes increased pulmonary endothelial permeability to albumin by reactive oxygen/nitrogen species. Pulm Pharmacol Ther. 2013;26:685-92 pubmed publisher
	TAMBOLI R, Hajri T, Jiang A, Marks Shulman P, Williams D, Clements R, et al. Reduction in inflammatory gene expression in skeletal muscle from Roux-en-Y gastric bypass patients randomized to omentectomy. PLoS ONE. 2011;6:e28577 pubmed publisher
	Williams S, Nowicki M, Liu F, Press R, Godlewski J, Abdel Rasoul M, et al. Indirubins decrease glioma invasion by blocking migratory phenotypes in both the tumor and stromal endothelial cell compartments. Cancer Res. 2011;71:5374-80 pubmed publisher
	Chen L, Wang Y, Tseng G. Compression alters kinase and phosphatase activity and tau and MAP2 phosphorylation transiently while inducing the fast adaptive dendritic remodeling of underlying cortical neurons. J Neurotrauma. 2010;27:1657-69 pubmed publisher

product information

Catalog Number :

05-413

Subcategory :

Cell Signaling

Product Name :

Anti-phospho-GSK3 (Tyr279/Tyr216) Antibody, clone 5G-2F

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P49840

Host Name :

Mouse

Antigen :

phospho-GSK3 (Tyr279/Tyr216)

Clone :

5G-2F

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-phospho-GSK3 (Tyr279/Tyr216) Antibody, clone 5G-2F

Cross Reactivity :

Human;Rat;Mouse;Dictyostelium;Yeast ( S. cerevisiae )

Background :

Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1

ALT Names :

GSK-3 alpha;glycogen synthase kinase 3 alpha

Immunogen :

Synthetic peptide (C-KQLLHGEPNVS [pY]ICSRY) corresponding to amino acids 203-219 derived from a region of the catalytic domain (between subdomain VII and VIII) of the Drosophila GSK3/shaggy enzyme coupled to ovalbumin.

Specificity :

This antibody recognizes GSK3 is constitutively phosphorylated at this Tyrosine residue in resting cells. This immunogen is identical in 15 of 17 residues in human and rat GSK3α (Tyr279) and GSK3β (Tyr216). The phosphotyrosine residue can be removed from GSK3 by treatment with PTP1B.

Package Size :

200 µg

Uses :

Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.