GSK3 Antibody, clone 4G-1E | EMD Millipore 05-412 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

GSK3 Antibody, clone 4G-1E

catalog :

05-412

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4G-1E

The same clone is also sold as:

Invitrogen: MA3-038

reactivity :

human, mouse, fruit fly

application :

western blot, immunocytochemistry

citations: 17

Published Application/Species/Sample/Dilution	Reference
western blot; fruit fly ; 1:5000; fig 5	Fischer R, Helfrich Forster C, Peschel N. GSK-3 Beta Does Not Stabilize Cryptochrome in the Circadian Clock of Drosophila. PLoS ONE. 2016;11:e0146571 pubmed publisher
western blot; fruit fly ; 1:1000	Cuesto G, Jordán Álvarez S, Enriquez Barreto L, Ferrús A, Morales M, Acebes A. GSK3β inhibition promotes synaptogenesis in Drosophila and mammalian neurons. PLoS ONE. 2015;10:e0118475 pubmed publisher
western blot; human	Loi M, Becherini P, Emionite L, Giacomini A, Cossu I, Destefanis E, et al. sTRAIL coupled to liposomes improves its pharmacokinetic profile and overcomes neuroblastoma tumour resistance in combination with Bortezomib. J Control Release. 2014;192:157-66 pubmed publisher
western blot; mouse	Li J, Swope D, Raess N, Cheng L, Muller E, Radice G. Cardiac tissue-restricted deletion of plakoglobin results in progressive cardiomyopathy and activation of {beta}-catenin signaling. Mol Cell Biol. 2011;31:1134-44 pubmed publisher
	White K, Grillo Hill B, Esquivel M, Peralta J, Bui V, Chire I, et al. β-Catenin is a pH sensor with decreased stability at higher intracellular pH. J Cell Biol. 2018;217:3965-3976 pubmed publisher
	Zhai L, Wang C, Chen Y, Zhou S, Li L. Rbm46 regulates mouse embryonic stem cell differentiation by targeting β-Catenin mRNA for degradation. PLoS ONE. 2017;12:e0172420 pubmed publisher
	Yahiaoui A, Meadows S, Sorensen R, Cui Z, Keegan K, Brockett R, et al. PI3Kδ inhibitor idelalisib in combination with BTK inhibitor ONO/GS-4059 in diffuse large B cell lymphoma with acquired resistance to PI3Kδ and BTK inhibitors. PLoS ONE. 2017;12:e0171221 pubmed publisher
	Yi H, Wang Z, Li X, Yin M, Wang L, Aldalbahi A, et al. Silica Nanoparticles Target a Wnt Signal Transducer for Degradation and Impair Embryonic Development in Zebrafish. Theranostics. 2016;6:1810-20 pubmed publisher
	Chen Y, Wu R, Yang X, Kou S, MacDougald O, Yu L, et al. Inhibiting DNA methylation switches adipogenesis to osteoblastogenesis by activating Wnt10a. Sci Rep. 2016;6:25283 pubmed publisher
	Tarafdar A, Dobbin E, Corrigan P, Freeburn R, Wheadon H. Canonical Wnt signaling promotes early hematopoietic progenitor formation and erythroid specification during embryonic stem cell differentiation. PLoS ONE. 2013;8:e81030 pubmed publisher
	Han K, Holder J, Schaaf C, Lu H, Chen H, Kang H, et al. SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties. Nature. 2013;503:72-7 pubmed publisher
	Ling L, Voskas D, Woodgett J. Activation of PDK-1 maintains mouse embryonic stem cell self-renewal in a PKB-dependent manner. Oncogene. 2013;32:5397-408 pubmed publisher
	Sarró E, Jacobs Cachá C, Itarte E, Meseguer A. A pharmacologically-based array to identify targets of cyclosporine A-induced toxicity in cultured renal proximal tubule cells. Toxicol Appl Pharmacol. 2012;258:275-87 pubmed publisher
	MacDonald B, Semenov M, Huang H, He X. Dissecting molecular differences between Wnt coreceptors LRP5 and LRP6. PLoS ONE. 2011;6:e23537 pubmed publisher
	Chen L, Wang Y, Tseng G. Compression alters kinase and phosphatase activity and tau and MAP2 phosphorylation transiently while inducing the fast adaptive dendritic remodeling of underlying cortical neurons. J Neurotrauma. 2010;27:1657-69 pubmed publisher
	Reintsch W, Habring Mueller A, Wang R, Schohl A, Fagotto F. beta-Catenin controls cell sorting at the notochord-somite boundary independently of cadherin-mediated adhesion. J Cell Biol. 2005;170:675-86 pubmed
	Eickholt B, Walsh F, Doherty P. An inactive pool of GSK-3 at the leading edge of growth cones is implicated in Semaphorin 3A signaling. J Cell Biol. 2002;157:211-7 pubmed

product information

Catalog Number :

05-412

Subcategory :

Cell Signaling

Product Name :

Anti-GSK3 Antibody, clone 4G-1E

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P49840

Host Name :

Mouse

Antigen :

GSK3

Clone :

4G-1E

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-GSK3 Antibody, clone 4G-1E

Cross Reactivity :

Vertebrates;Mouse;Rat;Human;Dictyostelium;Yeast ( S. pombe )

Background :

Glycogen synthase kinase-3 (GSK-3), a multifunctional serine/threonine kinase, is a key regulator of numerous signaling pathways. Two isoforms of GSK-3 are reported in mammals: a 51 kDa GSK-3a and a 47 kDa GSK-3b. These two isoforms exhibit about 98% homology in their kinase domains, but share only about 36% identity in the last 76 C-terminal amino acid residues. GSK-3a contains a glycine-rich extension at its N-terminus. A minor (~15% of total) splice variant of GSK-3b, GSK-3b2, has also been identified, which contains a 13-residue insert within the kinase domain. It exhibits reduced kinase activity towards tau protein compared with ‘unspliced’ GSK-3b. GSK-3b2 is localized primarily to neuronal cell bodies, unlike unspliced GSK-3b that is also found in neuronal processes. GSK-3b plays a key inhibitory role in the Wnt signaling pathway. Wnt genes encode a large family of secreted, cysteine-rich proteins that are important in development and in maintenance of adult tissues. Abnormalities in Wnt signaling are reported to promote both human dgenerative diseases and cancer.

ALT Names :

GSK-3 alpha;glycogen synthase kinase 3 alpha

Immunogen :

18 residue synthetic peptide (C-KQLLHGEP NVSYICSRY) corresponding to amino acids 203-219 derived from a region of the catalytic domain (between subdomain VII and VIII) of the Drosophila GSK3/shaggy enzyme, coupled to ovalbumin. Clone 4G-1E.

Specificity :

GSK3, α and β isoforms

Package Size :

100 µg

Uses :

Immunocytochemistry;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.