erbB-3/HER-3 Antibody, clone 2F12 | EMD Millipore 05-390 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

erbB-3/HER-3 Antibody, clone 2F12

catalog :

05-390

quantity :

200 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2F12

The same clone is also sold as:

Invitrogen: MA5-12675
LifeSpan Biosciences: LS-C87931, LS-C87932

reactivity :

human, mouse, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

citations: 16

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig S1	Buta C, Benabou E, Lequoy M, RÃ©gnault H, Wendum D, Meratbene F, et al. Heregulin-1ÃŸ and HER3 in hepatocellular carcinoma: status and regulation by insulin. J Exp Clin Cancer Res. 2016;35:126 pubmed publisher
western blot; human; fig 1	Cao K, Gong H, Qiu Z, Wen Q, Zhang B, Tang T, et al. Hepatitis B virus X protein reduces the stability of Nrdp1 to up-regulate ErbB3 in hepatocellular carcinoma cells. Tumour Biol. 2016;37:10375-82 pubmed publisher
immunoprecipitation; human; fig 3 western blot; human; fig 3	Wu Y, Wang L, Bao H, Zou S, Fu C, Gong H, et al. Nrdp1S, short variant of Nrdp1, inhibits human glioma progression by increasing Nrdp1-mediated ErbB3 ubiquitination and degradation. J Cell Mol Med. 2016;20:422-9 pubmed publisher
western blot; human; fig 2	Momeny M, Saunus J, Marturana F, McCart Reed A, Black D, Sala G, et al. Heregulin-HER3-HER2 signaling promotes matrix metalloproteinase-dependent blood-brain-barrier transendothelial migration of human breast cancer cell lines. Oncotarget. 2015;6:3932-46 pubmed
immunocytochemistry; human	Asp N, Pust S, Sandvig K. Flotillin depletion affects ErbB protein levels in different human breast cancer cells. Biochim Biophys Acta. 2014;1843:1987-96 pubmed publisher
immunohistochemistry - frozen section; human	Bauché S, Boerio D, Davoine C, Bernard V, Stum M, Bureau C, et al. Peripheral nerve hyperexcitability with preterminal nerve and neuromuscular junction remodeling is a hallmark of Schwartz-Jampel syndrome. Neuromuscul Disord. 2013;23:998-1009 pubmed publisher
western blot; human	Sahin O, Frohlich H, Löbke C, Korf U, Burmester S, Majety M, et al. Modeling ERBB receptor-regulated G1/S transition to find novel targets for de novo trastuzumab resistance. BMC Syst Biol. 2009;3:1 pubmed publisher
	Xiao Z, Sperl B, Ullrich A, Knyazev P. Metformin and salinomycin as the best combination for the eradication of NSCLC monolayer cells and their alveospheres (cancer stem cells) irrespective of EGFR, KRAS, EML4/ALK and LKB1 status. Oncotarget. 2014;5:12877-90 pubmed
	Tarafdar S, Poe J, Smithgall T. The accessory factor Nef links HIV-1 to Tec/Btk kinases in an Src homology 3 domain-dependent manner. J Biol Chem. 2014;289:15718-28 pubmed publisher
	Zamurs L, Pouliot N, Kusuma N, Nice E, Burgess A. Chain-specific antibodies for laminin-511. Growth Factors. 2013;31:209-19 pubmed publisher
	Fang S, Crews A, Chen W, Park J, Yin Q, Ren X, et al. MARCKS and HSP70 interactions regulate mucin secretion by human airway epithelial cells in vitro. Am J Physiol Lung Cell Mol Physiol. 2013;304:L511-8 pubmed publisher
	Bezler M, Hengstler J, Ullrich A. Inhibition of doxorubicin-induced HER3-PI3K-AKT signalling enhances apoptosis of ovarian cancer cells. Mol Oncol. 2012;6:516-29 pubmed publisher
	Deharvengt S, Marmarelis M, Korc M. Concomitant targeting of EGF receptor, TGF-beta and SRC points to a novel therapeutic approach in pancreatic cancer. PLoS ONE. 2012;7:e39684 pubmed publisher
	Fung C, Ke X, Brown A, Yu X, McKnight R, Lane R. Uteroplacental insufficiency alters rat hippocampal cellular phenotype in conjunction with ErbB receptor expression. Pediatr Res. 2012;72:2-9 pubmed publisher
	Camprecios G, Sánchez Vizcaíno E, Soley M, Ramirez I. Chronic ?-adrenergic stimulation increases ErbB receptors and cell proliferation in mouse kidney. Growth Factors. 2011;29:94-101 pubmed publisher
	Camprecios G, Lorita J, Pardina E, Peinado Onsurbe J, Soley M, Ramirez I. Expression, localization, and regulation of the neuregulin receptor ErbB3 in mouse heart. J Cell Physiol. 2011;226:450-5 pubmed publisher

product information

Catalog Number :

05-390

Subcategory :

Cell Signaling

Product Name :

Anti-erbB-3/HER-3 Antibody, clone 2F12

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P21860

Host Name :

Mouse

Antigen :

erbB-3/HER-3

Clone :

2F12

Conjugate :

Purified

Isotype :

IgG2aκ

Product Description :

Anti-erbB-3/HER-3 Antibody, clone 2F12

Cross Reactivity :

Human;Rat;Mouse;Bovine

Background :

The ErbB3 gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. ErbB3 is a membrane-bound protein which has a neuregulin binding domain but not an active kinase domain. It can therefore bind this ligand but cannot convey a signal into the cell via protein phosphorylation. However it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers including prostate, bladder and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported but they have not been thoroughly characterized.

ALT Names :

Tyrosine kinase-type cell surface receptor HER3;lethal congenital contracture syndrome 2;v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3;v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)

Immunogen :

Recombinant rat erbB-3/HER-3 oncoprotein expressed in insect cells and purified by metal chelating chromatography. The epitope maps between amino acid residues 1295-1323 of human erbB-3. Clone 2F12.

Specificity :

This antibody recognizes erbB-3/HER-3, Mr 185 kDa. Does not cross-react with erbB-1 (EGFR) and erbB-2/HER-2.

Package Size :

200 µg

Uses :

Immunocytochemistry;Immunohistochemistry;Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.