Cyclin B1 Antibody, clone GNS3 (8A5D12) | EMD Millipore 05-373 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Cyclin B1 Antibody, clone GNS3 (8A5D12)

catalog :

05-373

quantity :

200 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

GNS3

reactivity :

human, mouse

application :

western blot, immunoprecipitation

citations: 14

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 3c	Lee J, Sung J, Choi E, Yoon H, Kang B, Hong E, et al. C/EBPβ Is a Transcriptional Regulator of Wee1 at the G₂/M Phase of the Cell Cycle. Cells. 2019;8: pubmed publisher
western blot; human; loading ...; fig s1a	Cundell M, Hutter L, Nunes Bastos R, Poser E, Holder J, Mohammed S, et al. A PP2A-B55 recognition signal controls substrate dephosphorylation kinetics during mitotic exit. J Cell Biol. 2016;214:539-54 pubmed publisher
immunoprecipitation; human; fig 2b western blot; human; loading ...; fig 1b	Mukherjee S, Chakraborty P, Saha P. Phosphorylation of Ku70 subunit by cell cycle kinases modulates the replication related function of Ku heterodimer. Nucleic Acids Res. 2016;44:7755-65 pubmed publisher
western blot; human; 1:1000; fig 1	Iimori M, Watanabe S, Kiyonari S, Matsuoka K, Sakasai R, Saeki H, et al. Phosphorylation of EB2 by Aurora B and CDK1 ensures mitotic progression and genome stability. Nat Commun. 2016;7:11117 pubmed publisher
western blot; human; fig 5	Seidel C, Schnekenburger M, Mazumder A, Teiten M, Kirsch G, Dicato M, et al. 4-Hydroxybenzoic acid derivatives as HDAC6-specific inhibitors modulating microtubular structure and HSP90Î± chaperone activity against prostate cancer. Biochem Pharmacol. 2016;99:31-52 pubmed publisher
western blot; human	Bastos R, Cundell M, Barr F. KIF4A and PP2A-B56 form a spatially restricted feedback loop opposing Aurora B at the anaphase central spindle. J Cell Biol. 2014;207:683-93 pubmed publisher
western blot; mouse	Adeyemi R, Pintel D. Parvovirus-induced depletion of cyclin B1 prevents mitotic entry of infected cells. PLoS Pathog. 2014;10:e1003891 pubmed publisher
	Fuller M, Majumder K, Pintel D. Minute Virus of Mice Inhibits Transcription of the Cyclin B1 Gene during Infection. J Virol. 2017;91: pubmed publisher
	Saha J, Wang M, Cucinotta F. Investigation of switch from ATM to ATR signaling at the sites of DNA damage induced by low and high LET radiation. DNA Repair (Amst). 2013;12:1143-51 pubmed publisher
	Yajima H, Fujisawa H, Nakajima N, Hirakawa H, Jeggo P, Okayasu R, et al. The complexity of DNA double strand breaks is a critical factor enhancing end-resection. DNA Repair (Amst). 2013;12:936-46 pubmed publisher
	Ishitsuka Y, Kawachi Y, Maruyama H, Taguchi S, Fujisawa Y, Furuta J, et al. Pituitary tumor transforming gene 1 induces tumor necrosis factor-? production from keratinocytes: implication for involvement in the pathophysiology of psoriasis. J Invest Dermatol. 2013;133:2566-2575 pubmed publisher
	Al Dhaheri Y, Eid A, Abuqamar S, Attoub S, Khasawneh M, Aiche G, et al. Mitotic arrest and apoptosis in breast cancer cells induced by Origanum majorana extract: upregulation of TNF-? and downregulation of survivin and mutant p53. PLoS ONE. 2013;8:e56649 pubmed publisher
	Stingele S, Stoehr G, Peplowska K, Cox J, Mann M, Storchova Z. Global analysis of genome, transcriptome and proteome reveals the response to aneuploidy in human cells. Mol Syst Biol. 2012;8:608 pubmed publisher
	Llopis A, Salvador N, Ercilla A, Guaita Esteruelas S, Barrantes I, Gupta J, et al. The stress-activated protein kinases p38?/? and JNK1/2 cooperate with Chk1 to inhibit mitotic entry upon DNA replication arrest. Cell Cycle. 2012;11:3627-37 pubmed publisher

product information

Catalog Number :

05-373

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Cyclin B1 Antibody, clone GNS3 (8A5D12)

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P14635

Host Name :

Mouse

Antigen :

Cyclin B1

Clone :

GNS3 (8A5D12)

Conjugate :

Purified

Isotype :

mixed IgGs

Product Description :

Anti-Cyclin B1 Antibody, clone GNS3 (8A5D12)

Cross Reactivity :

Human;Mouse

Background :

Regulation of cell cycle progression in eukaryotic cells depends on the expression of cyclin proteins. These proteins are the regulatory subunits of the cell cycle-dependent kinases that are responsible for the phosphorylation of several cellular targets. The B cyclins, when combined with p34cdc2, a catalytic subunit of the cell cycle dependent kinase, form an enzyme that is active at the transition to the G2 phase, attaining maximum level at M phase. At that phase, the complex associates with condensed chromosomes in prophase and metaphase and is degraded at the metaphase/anaphase transition. Anti-Cyclin B1 may be used for the detection of cyclin B1 expression and for measuring relative differences in cyclin B1 level as a function of cell cycle phase.

ALT Names :

G2/mitotic-specific cyclin B1;cyclin B1

Immunogen :

Recombinant human cyclin B1

Specificity :

This antibody is specific for human cyclin B1, Mr 58 kDa. Does not cross-react with cyclin A or cyclin D.

Package Size :

200 µg

Uses :

Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.