Cyclin D1/2 Antibody, clone 5D4 | EMD Millipore 05-362 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Cyclin D1/2 Antibody, clone 5D4

catalog :

05-362

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5D4

The same clone is also sold as:

Enzo Life Sciences: ADI-KAM-CC200-E
LifeSpan Biosciences: LS-C179872

reactivity :

human, rat

application :

western blot, immunocytochemistry, immunoprecipitation

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:1000	Tang N, Lyu D, Liu T, Chen F, Jing S, Hao T, et al. Different Effects of p52SHC1 and p52SHC3 on the Cell Cycle of Neurons and Neural Stem Cells. J Cell Physiol. 2016;231:172-80 pubmed publisher
	Wang Y, Ma J, Chow S, Li C, Xiao Z, Feng R, et al. A potential antitumor ellagitannin, davidiin, inhibited hepatocellular tumor growth by targeting EZH2. Tumour Biol. 2014;35:205-12 pubmed publisher
	Gao Z, Godbout R. Serine phosphorylation regulates disabled-1 early isoform turnover independently of Reelin. Cell Signal. 2011;23:555-65 pubmed publisher
	Gherzi R, Trabucchi M, Ponassi M, Gallouzi I, Rosenfeld M, Briata P. Akt2-mediated phosphorylation of Pitx2 controls Ccnd1 mRNA decay during muscle cell differentiation. Cell Death Differ. 2010;17:975-83 pubmed publisher

product information

Catalog Number :

05-362

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Cyclin D1/2 Antibody, clone 5D4

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P24385

Host Name :

Mouse

Antigen :

Cyclin D1/2

Clone :

5D4

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-Cyclin D1/2 Antibody, clone 5D4

Cross Reactivity :

Human;Hamster;Mouse;Rat

Background :

During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by acting as regulatory subunits of the Cdks. Early in the G1 phase of the cell cycle, cyclin D1 induction is followed by cyclin E induction. This sequential progression is marked early on in G1 by the activation of Cdk4 and in mid to late G1 by the activation of Cdk2 and the hyperphosphorylation of pRB. The final transition into S phase is thought to be dependent on the increased expression and association of cyclin E and Cdk2. Cyclin D2 is also a G1 cyclin required for G1 phase progression and is a strong candidate for a proto oncogene. Cyclin D2 can phosphorylate pRB when associated with cdk 4 and / or cdk 6.

Immunogen :

Recombinant human cyclin D1

Specificity :

Recognizes p36 cyclin D1 and p34 cyclin D2; no cross-reactivity with cyclin A, B or D3.

Package Size :

100 μg

Uses :

Immunocytochemistry;Immunoprecipitation;Western Blotting

Storage :

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.