p21/WAF1/Cip1 Antibody | EMD Millipore 05-345 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

p21/WAF1/Cip1 Antibody

catalog :

05-345

quantity :

200 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CP36, CP74

reactivity :

human, mouse

application :

western blot, immunoprecipitation

citations: 31

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:4000; loading ...; fig 10a	Kalita B, Ranjan R, Singh A, Yashavarddhan M, Bajaj S, Gupta M. A Combination of Podophyllotoxin and Rutin Attenuates Radiation Induced Gastrointestinal Injury by Negatively Regulating NF-?B/p53 Signaling in Lethally Irradiated Mice. PLoS ONE. 2016;11:e0168525 pubmed publisher
western blot; human; fig 4	Funauchi Y, Tanikawa C, Yi Lo P, Mori J, Daigo Y, Takano A, et al. Regulation of iron homeostasis by the p53-ISCU pathway. Sci Rep. 2015;5:16497 pubmed publisher
western blot; human	Yang C, Chiang C, Chen C, Lee Y, Wu M, Tsou Y, et al. Identification and characterization of nuclear and nucleolar localization signals in 58-kDa microspherule protein (MSP58). J Biomed Sci. 2015;22:33 pubmed publisher
western blot; human	Sokka M, Rilla K, Miinalainen I, Pospiech H, SyvÃ¤oja J. High levels of TopBP1 induce ATR-dependent shut-down of rRNA transcription and nucleolar segregation. Nucleic Acids Res. 2015;43:4975-89 pubmed publisher
western blot; human; 1:2000	Zeng L, Holly J, Perks C. Effects of physiological levels of the green tea extract epigallocatechin-3-gallate on breast cancer cells. Front Endocrinol (Lausanne). 2014;5:61 pubmed publisher
western blot; human	Anderson V, Walton M, Eve P, Boxall K, Antoni L, Caldwell J, et al. CCT241533 is a potent and selective inhibitor of CHK2 that potentiates the cytotoxicity of PARP inhibitors. Cancer Res. 2011;71:463-72 pubmed publisher
	Calışkan B, Sinoplu E, İbiş K, Akhan Güzelcan E, Cetin Atalay R, Banoglu E. Synthesis and cellular bioactivities of novel isoxazole derivatives incorporating an arylpiperazine moiety as anticancer agents. J Enzyme Inhib Med Chem. 2018;33:1352-1361 pubmed publisher
	Noh J, Kim K, Idda M, Martindale J, Yang X, Abdelmohsen K, et al. GRSF1 suppresses cell senescence. Aging (Albany NY). 2018;10:1856-1866 pubmed publisher
	Shih C, Chang Y, Chen Y, Ma C, Chen H, Yang C, et al. The PPARγ-SETD8 axis constitutes an epigenetic, p53-independent checkpoint on p21-mediated cellular senescence. Aging Cell. 2017;16:797-813 pubmed publisher
	Benatti P, Basile V, Dolfini D, Belluti S, Tomei M, Imbriano C. NF-Y loss triggers p53 stabilization and apoptosis in HPV18-positive cells by affecting E6 transcription. Oncotarget. 2016;7:45901-45915 pubmed publisher
	Trino S, Iacobucci I, Erriquez D, Laurenzana I, De Luca L, Ferrari A, et al. Targeting the p53-MDM2 interaction by the small-molecule MDM2 antagonist Nutlin-3a: a new challenged target therapy in adult Philadelphia positive acute lymphoblastic leukemia patients. Oncotarget. 2016;7:12951-61 pubmed publisher
	Kim M, Min D, Wright G, Goldlust I, Annunziata C. Loss of compensatory pro-survival and anti-apoptotic modulator, IKKÎµ, sensitizes ovarian cancer cells to CHEK1 loss through an increased level of p21. Oncotarget. 2014;5:12788-802 pubmed
	Shi H, Fu C, Wang W, Li Y, Du S, Cao R, et al. The FGF-1-specific single-chain antibody scFv1C9 effectively inhibits breast cancer tumour growth and metastasis. J Cell Mol Med. 2014;18:2061-70 pubmed publisher
	Colciago A, Ruscica M, Mornati O, Piccolella M, Montagnani Marelli M, Eberini I, et al. In vitro chronic administration of ERbeta selective ligands and prostate cancer cell growth: hypotheses on the selective role of 3beta-adiol in AR-positive RV1 cells. Biomed Res Int. 2014;2014:801473 pubmed publisher
	Sourvinos G, Morou A, Sanidas I, Codruta I, Ezell S, Doxaki C, et al. The downregulation of GFI1 by the EZH2-NDY1/KDM2B-JARID2 axis and by human cytomegalovirus (HCMV) associated factors allows the activation of the HCMV major IE promoter and the transition to productive infection. PLoS Pathog. 2014;10:e1004136 pubmed publisher
	Chen Y, Lin H, Chen J, Tsai M, Hsieh H, Chang J, et al. CDKN1A-mediated responsiveness of MLL-AF4-positive acute lymphoblastic leukemia to Aurora kinase-A inhibitors. Int J Cancer. 2014;135:751-62 pubmed publisher
	Chuang M, Wu S, Tang S, Lai X, Lai H, Hsu K, et al. The HDAC inhibitor LBH589 induces ERK-dependent prometaphase arrest in prostate cancer via HDAC6 inactivation and down-regulation. PLoS ONE. 2013;8:e73401 pubmed publisher
	Lin Y, Huang A, Kuo C, Yang J, Lan Y, Yu C, et al. Induction of cell cycle arrest and apoptosis in human osteosarcoma U-2 OS cells by Solanum lyratum extracts. Nutr Cancer. 2013;65:469-79 pubmed publisher
	Gonon G, Groetz J, De Toledo S, Howell R, Fromm M, Azzam E. Nontargeted stressful effects in normal human fibroblast cultures exposed to low fluences of high charge, high energy (HZE) particles: kinetics of biologic responses and significance of secondary radiations. Radiat Res. 2013;179:444-57 pubmed publisher
	Wang Y, Sun Y, Qiao S. ADAM23 knockdown promotes neuronal differentiation of P19 embryonal carcinoma cells by up-regulating P27KIP1 expression. Cell Biol Int. 2012;36:1275-9 pubmed publisher
	Neault M, Mallette F, Vogel G, Michaud Levesque J, Richard S. Ablation of PRMT6 reveals a role as a negative transcriptional regulator of the p53 tumor suppressor. Nucleic Acids Res. 2012;40:9513-21 pubmed publisher
	Parolin C, Calonghi N, Presta E, Boga C, Caruana P, Naldi M, et al. Mechanism and stereoselectivity of HDAC I inhibition by (R)-9-hydroxystearic acid in colon cancer. Biochim Biophys Acta. 2012;1821:1334-40 pubmed publisher
	Lu H, Tung W, Yang J, Huang F, Lee C, Huang Y, et al. In vitro suppression of growth of murine WEHI-3 leukemia cells and in vivo promotion of phagocytosis in a leukemia mice model by indole-3-carbinol. J Agric Food Chem. 2012;60:7634-43 pubmed publisher
	Yu C, Ko F, Yu C, Lin C, Huang Y, Yang J, et al. Norcantharidin triggers cell death and DNA damage through S-phase arrest and ROS-modulated apoptotic pathways in TSGH 8301 human urinary bladder carcinoma cells. Int J Oncol. 2012;41:1050-60 pubmed publisher
	Hayashi M, Cesare A, Fitzpatrick J, Lazzerini Denchi E, Karlseder J. A telomere-dependent DNA damage checkpoint induced by prolonged mitotic arrest. Nat Struct Mol Biol. 2012;19:387-94 pubmed publisher
	Bouchet B, Fauvet F, Grelier G, Galmarini C, Puisieux A. p21(Cip1) regulates cell-substrate adhesion and interphase microtubule dynamics in untransformed human mammary epithelial cells. Eur J Cell Biol. 2011;90:631-41 pubmed publisher
	Autsavapromporn N, De Toledo S, Little J, Jay Gerin J, Harris A, Azzam E. The role of gap junction communication and oxidative stress in the propagation of toxic effects among high-dose ?-particle-irradiated human cells. Radiat Res. 2011;175:347-57 pubmed publisher
	Ruiz Miró M, Colomina N, Fernández R, Garí E, Gallego C, Aldea M. Translokin (Cep57) interacts with cyclin D1 and prevents its nuclear accumulation in quiescent fibroblasts. Traffic. 2011;12:549-62 pubmed publisher
	Napper J, Sollars V. 17-N-Allylamino-17-demethoxygeldanamycin induces a diverse response in human acute myelogenous cells. Leuk Res. 2010;34:1493-500 pubmed publisher
	Redmond K, Crawford N, Farmer H, D Costa Z, O Brien G, Buckley N, et al. T-box 2 represses NDRG1 through an EGR1-dependent mechanism to drive the proliferation of breast cancer cells. Oncogene. 2010;29:3252-62 pubmed publisher
	Yao C, Yang C, Chuang S, Yan J, Liu C, Chen S, et al. Targeting PML-RAR? and Oncogenic Signaling Pathways by Chinese Herbal Mixture Tien-Hsien Liquid in Acute Promyelocytic Leukemia NB4 Cells. Evid Based Complement Alternat Med. 2011;2011:984154 pubmed publisher

product information

Catalog Number :

05-345

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-p21/WAF1/Cip1 Antibody

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P38936

Host Name :

Mouse

Antigen :

p21/WAF1/Cip1

Clone :

CP36, CP74

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-p21/WAF1/Cip1 Antibody

Cross Reactivity :

Human;Mouse

Background :

Two families of cyclin dependent kinase inhibitors (CKIs) have been identified. The p21WAF1/Cip1 family inhibits all kinases involved in the G1/S transition. The p16INK4a family inhibits Cdk4 and Cdk6 specifically. The biochemical activities and patterns of expression of CKIs during development implicate these proteins as the primary effectors of signaling pathways that control cell cycle exit, an event that is critical for differentiation. Studies have shown that p21WAF1/Cip1 binds tightly to the G1 and S phase kinases, cyclin E/Cdk2, cyclin D/Cdk4, and cyclin A/Cdk2, and effectively inhibits their activity, whereas p21WAF1/Cip1 is a relatively poor inhibitor of the G2/M phase kinase cyclin B/Cdc2. In addition, p21WAF1/Cip1 inhibits proliferating cell nuclear antigen (PCNA), binds to and inhibits inactivation of Rb, which is essential for cell cycle progression, and may also interact with E2F and protect cells against p53-mediated apoptosis.

ALT Names :

CDK-interacting protein;CDK-interaction protein;DNA synthesis inhibitor;Melanoma differentiation-associated protein 6;cyclin-dependent kinase inhibitor 1A;melanoma differentiation associated protein 6;wild-type p53-activated fragment 1

Immunogen :

Recombinant full-length human Cip1 with a histidine tag.

Specificity :

Recognizes Cip1, MW 21 kDa

Package Size :

200 μg

Uses :

Western Blotting;Immunoprecipitation

Storage :

Stable for 1 year at from date of receipt.