Fas Antibody (human, neutralizing), clone ZB4 | EMD Millipore 05-338 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Fas Antibody (human, neutralizing), clone ZB4

catalog :

05-338

quantity :

100 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

ZB4

The same clone is also sold as:

LifeSpan Biosciences: LS-C179870
Enzo Life Sciences: ADI-AAM-227-E

reactivity :

human, mouse

application :

western blot, neutralization, flow cytometry, blocking or activating experiments

citations: 23

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; loading ...; fig 7e	Chen Y, Zheng Y, You X, Yu M, Fu G, Su X, et al. Kras Is Critical for B Cell Lymphopoiesis. J Immunol. 2016;196:1678-85 pubmed publisher
blocking or activating experiments; human	Lim D, Yawata N, Selva K, Li N, Tsai C, Yeong L, et al. The combination of type I IFN, TNF-Î±, and cell surface receptor engagement with dendritic cells enables NK cells to overcome immune evasion by dengue virus. J Immunol. 2014;193:5065-75 pubmed publisher
blocking or activating experiments; human	Li H, Pauza C. Critical roles for Akt kinase in controlling HIV envelope-mediated depletion of CD4 T cells. Retrovirology. 2013;10:60 pubmed publisher
	Shi L, Wang H, Wang F, Feng M, Wang M, Guan W. Effects of gastrokineâ€‘2 expression on gastric cancer cell apoptosis by activation of extrinsic apoptotic pathways. Mol Med Rep. 2014;10:2898-904 pubmed publisher
	Saxena N, Yadav P, Kumar O. The Fas/Fas ligand apoptotic pathway is involved in abrin-induced apoptosis. Toxicol Sci. 2013;135:103-18 pubmed publisher
	Praveen K, Saxena N. Crosstalk between Fas and JNK determines lymphocyte apoptosis after ionizing radiation. Radiat Res. 2013;179:725-36 pubmed publisher
	Su C, Lin H, Lin Y, Shan Y, Yang B. Expression of Th17-related genes in PHA/IL-2-activated human T cells by Fas signaling via caspase-1- and Stat3-dependent pathway. Cell Immunol. 2013;281:101-10 pubmed publisher
	Mizrahi K, Stein J, Yaniv I, Kaplan O, Askenasy N. TNF-? has tropic rather than apoptotic activity in human hematopoietic progenitors: involvement of TNF receptor-1 and caspase-8. Stem Cells. 2013;31:156-66 pubmed publisher
	Cheng S, Wu L, Hsieh Y, Wu C, Chan Y, Chang L, et al. Supercritical carbon dioxide extraction of aromatic turmerone from Curcuma longa Linn. induces apoptosis through reactive oxygen species-triggered intrinsic and extrinsic pathways in human hepatocellular carcinoma HepG2 cells. J Agric Food Chem. 2012;60:9620-30 pubmed
	García Santos G, Martin V, Rodriguez Blanco J, Herrera F, Casado Zapico S, Sánchez Sánchez A, et al. Fas/Fas ligand regulation mediates cell death in human Ewing's sarcoma cells treated with melatonin. Br J Cancer. 2012;106:1288-96 pubmed publisher
	Li H, Pauza C. HIV envelope-mediated, CCR5/?4?7-dependent killing of CD4-negative ?? T cells which are lost during progression to AIDS. Blood. 2011;118:5824-31 pubmed publisher
	Hsieh Y, Rao Y, Whang Peng J, Huang C, Shyue S, Hsu S, et al. Antcin B and its ester derivative from Antrodia camphorata induce apoptosis in hepatocellular carcinoma cells involves enhancing oxidative stress coincident with activation of intrinsic and extrinsic apoptotic pathway. J Agric Food Chem. 2011;59:10943-54 pubmed publisher
	Martínez Serra J, Maffiotte E, Martin J, Bex T, Navarro Palou M, Ros T, et al. Yondelis® (ET-743, Trabectedin) sensitizes cancer cell lines to CD95-mediated cell death: new molecular insight into the mechanism of action. Eur J Pharmacol. 2011;658:57-64 pubmed publisher
	Mavroudi I, Papadaki V, Pyrovolaki K, Katonis P, Eliopoulos A, Papadaki H. The CD40/CD40 ligand interactions exert pleiotropic effects on bone marrow granulopoiesis. J Leukoc Biol. 2011;89:771-83 pubmed publisher
	Buddingh E, Schilham M, Ruslan S, Berghuis D, Szuhai K, Suurmond J, et al. Chemotherapy-resistant osteosarcoma is highly susceptible to IL-15-activated allogeneic and autologous NK cells. Cancer Immunol Immunother. 2011;60:575-86 pubmed publisher
	Paunel Görgülü A, Flohe S, Scholz M, Windolf J, Lögters T. Increased serum soluble Fas after major trauma is associated with delayed neutrophil apoptosis and development of sepsis. Crit Care. 2011;15:R20 pubmed publisher
	Shin M, Shim J, Lee J, Chae W, Lee H, Morio T, et al. Qualitative and quantitative differences in the intensity of Fas-mediated intracellular signals determine life and death in T cells. Int J Hematol. 2010;92:262-70 pubmed publisher
	Xu Z, Ding T, Haridas V, Connolly F, Gutterman J. Avicin D, a plant triterpenoid, induces cell apoptosis by recruitment of Fas and downstream signaling molecules into lipid rafts. PLoS ONE. 2009;4:e8532 pubmed publisher
	Rubio S, Quintana J, Eiroa J, Triana J, Estevez F. Betuletol 3-methyl ether induces G(2)-M phase arrest and activates the sphingomyelin and MAPK pathways in human leukemia cells. Mol Carcinog. 2010;49:32-43 pubmed publisher
	Hestvik A, Skorstad G, Vartdal F, Holmøy T. Idiotope-specific CD4(+) T cells induce apoptosis of human oligodendrocytes. J Autoimmun. 2009;32:125-32 pubmed publisher
	Larrosa M, Tomas Barberan F, Espín J. The dietary hydrolysable tannin punicalagin releases ellagic acid that induces apoptosis in human colon adenocarcinoma Caco-2 cells by using the mitochondrial pathway. J Nutr Biochem. 2006;17:611-25 pubmed
	Clarke M, Savill J, Jones D, Noble B, Brown S. Compartmentalized megakaryocyte death generates functional platelets committed to caspase-independent death. J Cell Biol. 2003;160:577-87 pubmed
	Andreola G, Rivoltini L, Castelli C, Huber V, Perego P, Deho P, et al. Induction of lymphocyte apoptosis by tumor cell secretion of FasL-bearing microvesicles. J Exp Med. 2002;195:1303-16 pubmed

product information

Catalog Number :

05-338

Subcategory :

Apoptosis & Cancer

Product Name :

Anti-Fas Antibody (human, neutralizing), clone ZB4

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P25445

Host Name :

Mouse

Antigen :

Fas (human

Clone :

clone ZB4

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Fas Antibody (human, neutralizing), clone ZB4

Cross Reactivity :

Human

Background :

Fas (also known as Apo-1 and CD95) is a 40-50 kDa cell surface glycoprotein and a member of the TNF receptor family. Fas is an apotosis signalling molecule that forms the death inducing signalling complex (DISC) upon binding by the Fas ligand trimer on an adjacent cell. The Fas receptor also trimerizes, after which it is internalized and can then bind to the adaptor molecule FADD. FADD facilitates binding of caspase-8. which is activated to induce caspase-8 mediated apoptosis. Fas also plays a role in T cell mediated cytotoxicity and T cell development. Fas is found in the thymus, liver, heart, and ovary, where it is expressed on a minority of resting T and B cells and monocytes. The antagonistic ZB4 clone anti-Fas monoclonal has been used to block the apoptosis-inducing activity of the Anti-Fas CH-11 clone in multiple publications.

ALT Names :

APO-1 cell surface antigen;Apo-1 antigen;Apoptosis-mediating surface antigen FAS;CD95 antigen;FASLG receptor;Fas (TNF receptor superfamily, member 6);Fas AMA;Fas antigen;apoptosis antigen 1;tumor necrosis factor receptor superfamily member 6;tumor necrosis factor receptor superfamily, member 6

Immunogen :

Recombinant human Fas.

Specificity :

This antibody recognizes the human cell surface antigen Fas expressed in various human cells, including myeloid cells, T lympho-blastoid cells and diploid fibroblasts. It does not induce apoptosis in cell culture.

Package Size :

100 μg

Uses :

Western Blotting;Flow Cytometry;Neutralizing

Storage :

Stable for 1 year at from date of receipt.