ZAP-70 Antibody, clone 2F3.2 | EMD Millipore 05-253 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

ZAP-70 Antibody, clone 2F3.2

catalog :

05-253

quantity :

250 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2F3.2

The same clone is also sold as:

MyBioSource: MBS370118
LifeSpan Biosciences: LS-C87914, LS-C202918, LS-C312206, LS-C348627
Dako: M7303
Abnova: MAB13455, MAB13456
Invitrogen: MA5-12624
Novus Biologicals: NBP2-44502-0.1mg, NBP2-47774-0.1mg
OriGene: AM10148SU-N, TA327755

reactivity :

human

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

citations: 15

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 4	Klammt C, NovotnÃ¡ L, Li D, Wolf M, Blount A, Zhang K, et al. T cell receptor dwell times control the kinase activity of Zap70. Nat Immunol. 2015;16:961-9 pubmed publisher
flow cytometry; human	Szánthó E, Bhattoa H, Csobán M, Antal Szalmas P, Ujfalusi A, Kappelmayer J, et al. Serum thymidine kinase activity: analytical performance, age-related reference ranges and validation in chronic lymphocytic leukemia. PLoS ONE. 2014;9:e91647 pubmed publisher
immunohistochemistry - paraffin section; human; 1:80	Rossi M, Fuligni F, Ciccone M, Agostinelli C, Righi S, Luciani M, et al. Hsa-miR-15a and Hsa-miR-16-1 expression is not related to proliferation centers abundance and other prognostic factors in chronic lymphocytic leukemia. Biomed Res Int. 2013;2013:715391 pubmed publisher
western blot; human; fig 3c	Paster W, Brockmeyer C, Fu G, Simister P, de Wet B, Martinez RiaÃ±o A, et al. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. J Immunol. 2013;190:3749-56 pubmed publisher
western blot; human	Jarboui M, Bidoia C, Woods E, Roe B, Wynne K, Elia G, et al. Nucleolar protein trafficking in response to HIV-1 Tat: rewiring the nucleolus. PLoS ONE. 2012;7:e48702 pubmed publisher
flow cytometry; human	Bojarska Junak A, Giannopoulos K, Kowal M, Dmoszynska A, Rolinski J. Comparison of methods for determining zeta-chain associated protein - 70 (ZAP-70) expression in patients with B-cell chronic lymphocytic leukemia (B-CLL). Cytometry B Clin Cytom. 2006;70:293-301 pubmed
	Negrini M, Cutrona G, Bassi C, Fabris S, Zagatti B, Colombo M, et al. microRNAome expression in chronic lymphocytic leukemia: comparison with normal B-cell subsets and correlations with prognostic and clinical parameters. Clin Cancer Res. 2014;20:4141-53 pubmed publisher
	Gentile M, Cutrona G, Mosca L, Matis S, Fabris S, Lionetti M, et al. Prospective validation of a risk score based on biological markers for predicting progression free survival in Binet stage A chronic lymphocytic leukemia patients: results of the multicenter O-CLL1-GISL study. Am J Hematol. 2014;89:743-50 pubmed publisher
	Morabito F, Mosca L, Cutrona G, Agnelli L, Tuana G, Ferracin M, et al. Clinical monoclonal B lymphocytosis versus Rai 0 chronic lymphocytic leukemia: A comparison of cellular, cytogenetic, molecular, and clinical features. Clin Cancer Res. 2013;19:5890-900 pubmed publisher
	Gentile M, Cutrona G, Fabris S, Pesce E, Baldini L, Di Raimondo F, et al. Total body computed tomography scan in the initial work-up of Binet stage A chronic lymphocytic leukemia patients: Results of the prospective, multicenter O-CLL1-GISL study. Am J Hematol. 2013;88:539-44 pubmed publisher
	Best O, Che Y, Singh N, Forsyth C, Christopherson R, Mulligan S. The Hsp90 inhibitor SNX-7081 synergizes with and restores sensitivity to fludarabine in chronic lymphocytic leukemia cells with lesions in the TP53 pathway: a potential treatment strategy for fludarabine refractory disease. Leuk Lymphoma. 2012;53:1367-75 pubmed publisher
	Brockmeyer C, Paster W, Pepper D, Tan C, Trudgian D, McGowan S, et al. T cell receptor (TCR)-induced tyrosine phosphorylation dynamics identifies THEMIS as a new TCR signalosome component. J Biol Chem. 2011;286:7535-47 pubmed publisher
	Sulda M, Kuss B, Hall R, Bailey S, Macardle P. Clinical utility of molecular and flow cytometric markers in chronic lymphocytic leukaemia. Intern Med J. 2012;42:137-46 pubmed publisher
	Krysov S, Potter K, Mockridge C, Coelho V, Wheatley I, Packham G, et al. Surface IgM of CLL cells displays unusual glycans indicative of engagement of antigen in vivo. Blood. 2010;115:4198-205 pubmed publisher
	Grever M, Lucas D, Dewald G, Neuberg D, Reed J, Kitada S, et al. Comprehensive assessment of genetic and molecular features predicting outcome in patients with chronic lymphocytic leukemia: results from the US Intergroup Phase III Trial E2997. J Clin Oncol. 2007;25:799-804 pubmed

product information

Catalog Number :

05-253

Subcategory :

Cell Signaling

Product Name :

Anti-ZAP-70 Antibody, clone 2F3.2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P43403

Host Name :

Mouse

Antigen :

ZAP-70

Clone :

2F3.2

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-ZAP-70 Antibody, clone 2F3.2

Cross Reactivity :

Human

Background :

Zeta-chain-associated protein kinase 70. A member of the protein tyrosine kinase family, ZAP-70 is normally expressed in T cells and natural killer cells and has a critical role in the initiation of T-cell signaling. ZAP-70 is expressed in T cells and tumors of T-cell lineage. A high level of ZAP-70 expression appears restricted to T-cell proliferative diseases and a subgroup of chronic lymphocytic leukemia (CLL). The ZAP-70 gene is in chromosome 2q12.

Immunogen :

GST-fusion to tandem SH2 domains of human ZAP-70 corresponding to residues 1-254

Specificity :

Recognizes human ZAP-70, does not recognize murine ZAP-70.

Package Size :

250 μg

Uses :

Flow Cytometry;Immunohistochemistry;Immunoprecipitation;Western Blotting

Storage :

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.