EF1α Antibody, clone CBP-KK1 | EMD Millipore 05-235 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

EF1α Antibody, clone CBP-KK1

catalog :

05-235

quantity :

200 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CBP-KK1

The same clone is also sold as:

Santa Cruz Biotechnology: sc-21758

reactivity :

Trypanosoma brucei, human, mouse, chicken, pigs

application :

western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

citations: 31

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 6a	Ricker C, Berlow N, Crawford K, Georgopapadakos T, Huelskamp A, Woods A, et al. Undifferentiated small round cell sarcoma in a young male: a case report. Cold Spring Harb Mol Case Stud. 2020;6: pubmed publisher
western blot; mouse; 1:5000; loading ...; fig 6e western blot; human; 1:5000; loading ...; fig 1e, 3a, s4g	Liu S, Hausmann S, CARLSON S, Fuentes M, Francis J, Pillai R, et al. METTL13 Methylation of eEF1A Increases Translational Output to Promote Tumorigenesis. Cell. 2019;176:491-504.e21 pubmed publisher
immunohistochemistry - frozen section; chicken; 1:1000; loading ...; tbl 1 western blot; chicken; 1:1000; loading ...; tbl 1	Sakano H, Zorio D, Wang X, Ting Y, Noble W, MacCoss M, et al. Proteomic analyses of nucleus laminaris identified candidate targets of the fragile X mental retardation protein. J Comp Neurol. 2017;525:3341-3359 pubmed publisher
western blot; mouse; loading ...; fig 2h	Akiel M, Guo C, Li X, Rajasekaran D, Mendoza R, Robertson C, et al. IGFBP7 Deletion Promotes Hepatocellular Carcinoma. Cancer Res. 2017;77:4014-4025 pubmed publisher
immunocytochemistry; human; fig 8c	Malecki J, Aileni V, Ho A, Schwarz J, Moen A, Sørensen V, et al. The novel lysine specific methyltransferase METTL21B affects mRNA translation through inducible and dynamic methylation of Lys-165 in human eukaryotic elongation factor 1 alpha (eEF1A). Nucleic Acids Res. 2017;45:4370-4389 pubmed publisher
western blot; Trypanosoma brucei; 1:10,000; fig 3c	Mani J, Rout S, Desy S, Schneider A. Mitochondrial protein import - Functional analysis of the highly diverged Tom22 orthologue of Trypanosoma brucei. Sci Rep. 2017;7:40738 pubmed publisher
western blot; Trypanosoma brucei; 1:10,000; loading ...; fig 3a	Harsman A, Oeljeklaus S, Wenger C, Huot J, Warscheid B, Schneider A. The non-canonical mitochondrial inner membrane presequence translocase of trypanosomatids contains two essential rhomboid-like proteins. Nat Commun. 2016;7:13707 pubmed publisher
immunoprecipitation; mouse; loading ...; fig 5a western blot; mouse; 1:2000; loading ...; fig 5a	Silva R, Sattlegger E, Castilho B. Perturbations in actin dynamics reconfigure protein complexes that modulate GCN2 activity and promote an eIF2 response. J Cell Sci. 2016;129:4521-4533 pubmed
western blot; human; loading ...; fig 3c	Park O, Park J, Yu M, An H, Ko J, Kim Y. Identification and molecular characterization of cellular factors required for glucocorticoid receptor-mediated mRNA decay. Genes Dev. 2016;30:2093-2105 pubmed
immunoprecipitation; human; fig 2 western blot; human; fig 2	Altuntas S, Rossin F, Marsella C, D Eletto M, Diaz Hidalgo L, Farrace M, et al. The transglutaminase type 2 and pyruvate kinase isoenzyme M2 interplay in autophagy regulation. Oncotarget. 2015;6:44941-54 pubmed publisher
western blot; human; fig 1a	Chiu K, Kuo T, Kuok Q, Lin Y, Hua C, Lin C, et al. ADAM9 enhances CDCP1 protein expression by suppressing miR-218 for lung tumor metastasis. Sci Rep. 2015;5:16426 pubmed publisher
western blot; Trypanosoma brucei; 1:10,000; fig 1	Mani J, Desy S, Niemann M, Chanfon A, Oeljeklaus S, Pusnik M, et al. Mitochondrial protein import receptors in Kinetoplastids reveal convergent evolution over large phylogenetic distances. Nat Commun. 2015;6:6646 pubmed publisher
western blot; mouse; 1:2000	Uys J, McGuier N, Gass J, Griffin W, Ball L, Mulholland P. Chronic intermittent ethanol exposure and withdrawal leads to adaptations in nucleus accumbens core postsynaptic density proteome and dendritic spines. Addict Biol. 2016;21:560-74 pubmed publisher
immunoprecipitation; mouse	Saikia M, Krokowski D, Guan B, Ivanov P, Parisien M, Hu G, et al. Genome-wide identification and quantitative analysis of cleaved tRNA fragments induced by cellular stress. J Biol Chem. 2012;287:42708-25 pubmed publisher
western blot; pigs ; fig 4	Yun Hong Y, Chih Fan C, Chia Wei C, Yen Chung C. A study of the spatial protein organization of the postsynaptic density isolated from porcine cerebral cortex and cerebellum. Mol Cell Proteomics. 2011;10:M110.007138 pubmed publisher
	von Känel C, Muñoz Gómez S, Oeljeklaus S, Wenger C, Warscheid B, Wideman J, et al. Homologue replacement in the import motor of the mitochondrial inner membrane of trypanosomes. elife. 2020;9: pubmed publisher
	Marques C, Tiengwe C, Lemgruber L, Damasceno J, Scott A, Paape D, et al. Diverged composition and regulation of the Trypanosoma brucei origin recognition complex that mediates DNA replication initiation. Nucleic Acids Res. 2016;44:4763-84 pubmed publisher
	Tomassy G, Morello N, Calcagno E, Giustetto M. Developmental abnormalities of cortical interneurons precede symptoms onset in a mouse model of Rett syndrome. J Neurochem. 2014;131:115-27 pubmed publisher
	Sher Y, Wang L, Chuang L, Tsai M, Kuo T, Huang C, et al. ADAM9 up-regulates N-cadherin via miR-218 suppression in lung adenocarcinoma cells. PLoS ONE. 2014;9:e94065 pubmed publisher
	Schulz I, Engel C, Niestroj A, Kehlen A, Rahfeld J, Kleinschmidt M, et al. A non-canonical function of eukaryotic elongation factor 1A1: regulation of interleukin-6 expression. Biochim Biophys Acta. 2014;1843:965-75 pubmed publisher
	Matassa D, Amoroso M, Agliarulo I, Maddalena F, Sisinni L, Paladino S, et al. Translational control in the stress adaptive response of cancer cells: a novel role for the heat shock protein TRAP1. Cell Death Dis. 2013;4:e851 pubmed publisher
	Darland T, Mauch J, Meier E, Hagan S, Dowling J, Darland D. Sulpiride, but not SCH23390, modifies cocaine-induced conditioned place preference and expression of tyrosine hydroxylase and elongation factor 1? in zebrafish. Pharmacol Biochem Behav. 2012;103:157-67 pubmed publisher
	Watabe Rudolph M, Song Z, Lausser L, Schnack C, Begus Nahrmann Y, Scheithauer M, et al. Chitinase enzyme activity in CSF is a powerful biomarker of Alzheimer disease. Neurology. 2012;78:569-77 pubmed publisher
	Song Z, von Figura G, Liu Y, Kraus J, Torrice C, Dillon P, et al. Lifestyle impacts on the aging-associated expression of biomarkers of DNA damage and telomere dysfunction in human blood. Aging Cell. 2010;9:607-15 pubmed publisher
	Schneider H, Dabauvalle M, Wilken N, Scheer U. Visualizing protein interactions involved in the formation of the 42S RNP storage particle of Xenopus oocytes. Biol Cell. 2010;102:469-78 pubmed publisher
	Murthi A, Shaheen H, Huang H, Preston M, Lai T, Phizicky E, et al. Regulation of tRNA bidirectional nuclear-cytoplasmic trafficking in Saccharomyces cerevisiae. Mol Biol Cell. 2010;21:639-49 pubmed publisher
	Gillardon F. Interaction of elongation factor 1-alpha with leucine-rich repeat kinase 2 impairs kinase activity and microtubule bundling in vitro. Neuroscience. 2009;163:533-9 pubmed publisher
	Tash J, Chakrasali R, Jakkaraj S, Hughes J, Smith S, Hornbaker K, et al. Gamendazole, an orally active indazole carboxylic acid male contraceptive agent, targets HSP90AB1 (HSP90BETA) and EEF1A1 (eEF1A), and stimulates Il1a transcription in rat Sertoli cells. Biol Reprod. 2008;78:1139-52 pubmed publisher
	Xing Y, Chaudry Q, Shen C, Kong K, Zhau H, Chung L, et al. Bioconjugated quantum dots for multiplexed and quantitative immunohistochemistry. Nat Protoc. 2007;2:1152-65 pubmed
	Vande Walle L, Van Damme P, Lamkanfi M, Saelens X, Vandekerckhove J, Gevaert K, et al. Proteome-wide Identification of HtrA2/Omi Substrates. J Proteome Res. 2007;6:1006-15 pubmed
	Thelemann A, Petti F, Griffin G, Iwata K, Hunt T, Settinari T, et al. Phosphotyrosine signaling networks in epidermal growth factor receptor overexpressing squamous carcinoma cells. Mol Cell Proteomics. 2005;4:356-76 pubmed

product information

Catalog Number :

05-235

Subcategory :

Protein Trafficking

Product Name :

Anti-EF1α Antibody, clone CBP-KK1

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P68104

Host Name :

Mouse

Antigen :

EF1α

Clone :

CBP-KK1

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-EF1α Antibody, clone CBP-KK1

Cross Reactivity :

Bacteria;Mammals;Yeast ( S. cerevisiae )

Background :

Human eukaryotic translation elongation factor 1 alpha has two isoforms, alpha 1 and alpha 2. The alpha 1 isoform gene encodes a subunit of the elongation factor-1 complex which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. The alpha 1 isoform is expressed in brain, placenta, lung, liver, kidney, and pancreas. The other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. The alpha 1 isoform is identified as an autoantigen in 66% of patients with Felty syndrome. The gene for the alpha 1 isoform has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. The gene for the alpha 2 may be critical in the development of ovarian cancer.

ALT Names :

CTCL tumor antigen;EF1a-like protein;Elongation factor 1 A-1;Elongation factor Tu;Leukocyte receptor cluster member 7;cervical cancer suppressor 3;elongation factor 1 alpha subunit;elongation factor 1-alpha;eukaryotic translation elongation factor 1 alpha 1;eukaryotic translation elongation factor 1 alpha 1-like 14;glucocorticoid receptor AF-1 specific elongation factor;leukocyte receptor cluster (LRC) member 7;prostate tumor-inducing protein 1;translation elongation factor 1 alpha 1-like 14

Immunogen :

Crude calmodulin-binding proteins from Trypanosoma brucei isolated using calmodulin-affinity chromatography. Clone CBP-KK1.

Specificity :

This antibody recognizes EF-1α (Elongation factor-1 alpha), Mr 53 kDa. Occasionally, a 38 kDa protein is also detected in A431 cell lysate.

Package Size :

200 µg

Uses :

Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.