p53 Antibody, clone BP53-12 | EMD Millipore 05-224 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

p53 Antibody, clone BP53-12

catalog :

05-224

quantity :

200 μL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BP53-12

The same clone is also sold as:

LifeSpan Biosciences: LS-C358011, LS-C153050, LS-C122264, LS-B431, LS-C45758, LS-C46336, LS-C41085
Novus Biologicals: NBP2-29453, NBP2-33074
BioLegend: 628201, 628202
Santa Cruz Biotechnology: sc-263
Rockland Immunochemicals: 200-301-174
Invitrogen: A15733, MA1-19551, MA1-19055
Abnova: MAB11254, MAB4528
US Biological: 138582
OriGene: BM4079F, BM4079S, AM10034FC-N, AM10034FC-S, AM10034PU-S, TA327920, AM10034PU-N
MilliporeSigma: P5813
Boster: MA1078
Exbio: 1F-114-C025, 1F-114-C100, 11-114-C100, 11-114-C025

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, western blot knockout validation

citations: 12

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; human; loading ...; fig 2i	Zhang Q, Jin R, Zhang X, Sheng J, Yu F, Tan R, et al. The putative oncotarget CSN5 controls a transcription-uncorrelated p53-mediated autophagy implicated in cancer cell survival under curcumin treatment. Oncotarget. 2016;7:69688-69702 pubmed publisher
immunocytochemistry; human; 1:150; fig 5 western blot; human; fig 6	Waye S, Naeem A, Choudhry M, Parasido E, Tricoli L, Sivakumar A, et al. The p53 tumor suppressor protein protects against chemotherapeutic stress and apoptosis in human medulloblastoma cells. Aging (Albany NY). 2015;7:854-68 pubmed
western blot; human	Yang C, Chiang C, Chen C, Lee Y, Wu M, Tsou Y, et al. Identification and characterization of nuclear and nucleolar localization signals in 58-kDa microspherule protein (MSP58). J Biomed Sci. 2015;22:33 pubmed publisher
immunocytochemistry; human; 1:1000	Gillory L, Stewart J, Megison M, Waters A, Beierle E. Focal adhesion kinase and p53 synergistically decrease neuroblastoma cell survival. J Surg Res. 2015;196:339-49 pubmed publisher
	Ringer L, Sirajuddin P, Tricoli L, Waye S, Choudhry M, Parasido E, et al. The induction of the p53 tumor suppressor protein bridges the apoptotic and autophagic signaling pathways to regulate cell death in prostate cancer cells. Oncotarget. 2014;5:10678-91 pubmed
	Mattiolo P, Barbero Farran A, Yuste V, Boix J, Ribas J. 2-Phenylethynesulfonamide (PES) uncovers a necrotic process regulated by oxidative stress and p53. Biochem Pharmacol. 2014;91:301-11 pubmed publisher
	Roy M, Mukherjee S. Reversal of resistance towards cisplatin by curcumin in cervical cancer cells. Asian Pac J Cancer Prev. 2014;15:1403-10 pubmed
	Klippel S, Jakubikova J, Delmore J, Ooi M, McMillin D, Kastritis E, et al. Methyljasmonate displays in vitro and in vivo activity against multiple myeloma cells. Br J Haematol. 2012;159:340-51 pubmed publisher
	Madison D, Stauffer D, Lundblad J. The PARP inhibitor PJ34 causes a PARP1-independent, p21 dependent mitotic arrest. DNA Repair (Amst). 2011;10:1003-13 pubmed publisher
	Nelson E, Folkmann A, Henry M, DeMali K. Vinculin activators target integrins from within the cell to increase melanoma sensitivity to chemotherapy. Mol Cancer Res. 2011;9:712-23 pubmed publisher
	Al Rashid S, Harding S, Law C, Coackley C, Bristow R. Protein-protein interactions occur between p53 phosphoforms and ATM and 53BP1 at sites of exogenous DNA damage. Radiat Res. 2011;175:588-98 pubmed publisher
	Sermeus A, Cosse J, Crespin M, Mainfroid V, de Longueville F, Ninane N, et al. Hypoxia induces protection against etoposide-induced apoptosis: molecular profiling of changes in gene expression and transcription factor activity. Mol Cancer. 2008;7:27 pubmed publisher

product information

Catalog Number :

05-224

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-p53 Antibody, clone BP53-12

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P04637

Host Name :

Mouse

Antigen :

p53

Clone :

BP53-12

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-p53 Antibody, clone BP53-12

Cross Reactivity :

Human

Background :

The human wild-type p53 protein is a 393 amino acid nuclear phosphoprotein, present in the nucleus of all normal mammalian cells where it appears to be involved in the regulation of cell proliferation. The normal protein has a very short half-life and is present in only minute amounts in normal tissues and cells. In contrast, mutant p53 protein produced by malignant cells is usually a product of a point mutation in the p53 gene leading to substitution of a single amino acid that significantly prolongs the half-life of the protein. The accumulation of high levels of p53 is a potential novel marker for malignancy.

Immunogen :

Recombinant human wild type p53

Specificity :

Recognizes p53.

Package Size :

200 μL

Uses :

Immunohistochemistry;Immunoprecipitation;Western Blotting

Storage :

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.