PI3 Kinase Antibody, p85, N-SH3, clone AB6 | EMD Millipore 05-212 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PI3 Kinase Antibody, p85, N-SH3, clone AB6

catalog :

05-212

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AB6

The same clone is also sold as:

LifeSpan Biosciences: LS-C179866

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation

citations: 6

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig s13a	Cao X, Kaneko T, Li J, Liu A, Voss C, Li S. A phosphorylation switch controls the spatiotemporal activation of Rho GTPases in directional cell migration. Nat Commun. 2015;6:7721 pubmed publisher
western blot; human; tbl s3	Cheng H, Liang Y, Kuo Y, Chuu C, Lin C, Lee M, et al. Identification of thioridazine, an antipsychotic drug, as an antiglioblastoma and anticancer stem cell agent using public gene expression data. Cell Death Dis. 2015;6:e1753 pubmed publisher
western blot; human; 1:1000	Elliott V, Rychahou P, Zaytseva Y, Evers B. Activation of c-Met and upregulation of CD44 expression are associated with the metastatic phenotype in the colorectal cancer liver metastasis model. PLoS ONE. 2014;9:e97432 pubmed publisher
	Ruiz Sáenz A, Dreyer C, Campbell M, Steri V, Gulizia N, Moasser M. HER2 Amplification in Tumors Activates PI3K/Akt Signaling Independent of HER3. Cancer Res. 2018;78:3645-3658 pubmed publisher
	Thorpe L, Spangle J, Ohlson C, Cheng H, Roberts T, Cantley L, et al. PI3K-p110? mediates the oncogenic activity induced by loss of the novel tumor suppressor PI3K-p85?. Proc Natl Acad Sci U S A. 2017;114:7095-7100 pubmed publisher
	Mukherjee A, Badal Y, Nguyen X, Miller J, Chenna A, Tahir H, et al. Profiling the HER3/PI3K pathway in breast tumors using proximity-directed assays identifies correlations between protein complexes and phosphoproteins. PLoS ONE. 2011;6:e16443 pubmed publisher

product information

Catalog Number :

05-212

Subcategory :

Cell Signaling

Product Name :

Anti-PI3 Kinase Antibody, p85, N-SH3, clone AB6

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P27986

Host Name :

Mouse

Antigen :

PI3 Kinase

Clone :

AB6

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

Anti-PI3 Kinase Antibody, p85, N-SH3, clone AB6

Cross Reactivity :

Human;Mouse;Rat

Background :

The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that is thought to phosphorylate second messengers in growth signaling pathways. PI3K exists as a heterodimer of catalytic 110 kDa (p110) and regulatory 85 kDa (p85, p50 or p55) subunits. The p85 unit consists of two closely related proteins: p85 alpha, which binds tightly to the catalytic subunit and p85 beta, a protein of unknown function. They share 62% amino acid sequence identity. p85 alpha lacks PI3 kinase activity, but may serve as regulator of the catalytic subunit, p110, by acting as the link between PI3K and the ligand activated receptor. Both isoforms bind to activated receptors and serve as tyrosine kinase substrates.

ALT Names :

PI3-kinase p85 subunit alpha;PtdIns-3-kinase p85-alpha;phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha);phosphatidylinositol 3-kinase, regulatory, 1;phosphatidylinositol 3-kinase-associated p-85 alpha;phosphoinositide-3-kinase, regulatory subunit 1 (alpha);phosphoinositide-3-kinase, regulatory subunit 1 (p85 alpha);phosphoinositide-3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)

Immunogen :

Recombinant human p85 alpha PI3 Kinase expressed in E. Coli. Clone AB6.

Specificity :

This antibody recognizes p85 alpha subunit of PI3 Kinase; does not recognize p85 beta subunit.

Package Size :

100 µg

Uses :

Immunocytochemistry;Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.