This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Fas Antibody (human, activating), clone CH11
catalog :
05-201
quantity :
50 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
CH11

The same clone is also sold as:
reactivity :
human, mouse, bovine
application :
western blot, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, blocking or activating experiments
citations: 104
Published Application/Species/Sample/DilutionReference
  • blocking or activating experiments; human; loading ...; fig 4a
Sommer A, Kordowski F, Büch J, Maretzky T, Evers A, Andrä J, et al. Phosphatidylserine exposure is required for ADAM17 sheddase function. Nat Commun. 2016;7:11523 pubmed publisher
  • flow cytometry; human; loading ...; fig 2b
Trisdale S, Schwab N, Hou X, Davis J, Townson D. Molecular manipulation of keratin 8/18 intermediate filaments: modulators of FAS-mediated death signaling in human ovarian granulosa tumor cells. J Ovarian Res. 2016;9:8 pubmed publisher
  • blocking or activating experiments; human
Gill R, Lanni L, Jen K, McCabe M, ROSENSPIRE A. Docosahexaenoic acid counteracts attenuation of CD95-induced cell death by inorganic mercury. Toxicol Appl Pharmacol. 2015;282:61-7 pubmed publisher
  • flow cytometry; human
Park S, Park J, Kim Y, Song S, Kwon H, Lee Y. Suberoylanilide hydroxamic acid induces ROS-mediated cleavage of HSP90 in leukemia cells. Cell Stress Chaperones. 2015;20:149-57 pubmed publisher
  • blocking or activating experiments; human
Boyd Tressler A, Penuela S, Laird D, Dubyak G. Chemotherapeutic drugs induce ATP release via caspase-gated pannexin-1 channels and a caspase/pannexin-1-independent mechanism. J Biol Chem. 2014;289:27246-63 pubmed publisher
  • western blot; human; 1:500
Chondrogiannis G, Kastamoulas M, Kanavaros P, Vartholomatos G, Bai M, Baltogiannis D, et al. Cytokine effects on cell viability and death of prostate carcinoma cells. Biomed Res Int. 2014;2014:536049 pubmed publisher
  • western blot; human
Potu H, Peterson L, Pal A, Verhaegen M, Cao J, Talpaz M, et al. Usp5 links suppression of p53 and FAS levels in melanoma to the BRAF pathway. Oncotarget. 2014;5:5559-69 pubmed
  • flow cytometry; human
Gao B, Moore C, Porcheray F, Rong C, Abidoglu C, DeVito J, et al. Pretransplant IgG reactivity to apoptotic cells correlates with late kidney allograft loss. Am J Transplant. 2014;14:1581-91 pubmed publisher
  • immunoprecipitation; human; 1 ug/ml
  • western blot; human
Li C, Egloff A, Sen M, Grandis J, Johnson D. Caspase-8 mutations in head and neck cancer confer resistance to death receptor-mediated apoptosis and enhance migration, invasion, and tumor growth. Mol Oncol. 2014;8:1220-30 pubmed publisher
  • blocking or activating experiments; human; 15 ug/ml
Noviello M, Tedesco F, Bondanza A, Tonlorenzi R, Rosaria Carbone M, Gerli M, et al. Inflammation converts human mesoangioblasts into targets of alloreactive immune responses: implications for allogeneic cell therapy of DMD. Mol Ther. 2014;22:1342-1352 pubmed publisher
  • flow cytometry; human
  • western blot; human
Villa Morales M, Cobos M, González Gugel E, Alvarez Iglesias V, Martinez B, Piris M, et al. FAS system deregulation in T-cell lymphoblastic lymphoma. Cell Death Dis. 2014;5:e1110 pubmed publisher
  • immunocytochemistry; human; 1:1000
Li H, Zhou Y, Zhao A, Qiu Y, Xie G, Jiang Q, et al. Asymmetric dimethylarginine attenuates serum starvation-induced apoptosis via suppression of the Fas (APO-1/CD95)/JNK (SAPK) pathway. Cell Death Dis. 2013;4:e830 pubmed publisher
  • blocking or activating experiments; human; 200 ng/ml
Porcheray F, Fraser J, Gao B, McColl A, DeVito J, Dargon I, et al. Polyreactive antibodies developing amidst humoral rejection of human kidney grafts bind apoptotic cells and activate complement. Am J Transplant. 2013;13:2590-600 pubmed publisher
  • immunocytochemistry; human; 500 ng/mL
Lolli F, Martini H, Citro A, Franceschini D, Portaccio E, Amato M, et al. Increased CD8+ T cell responses to apoptotic T cell-associated antigens in multiple sclerosis. J Neuroinflammation. 2013;10:94 pubmed publisher
  • blocking or activating experiments; human
Li H, Pauza C. Critical roles for Akt kinase in controlling HIV envelope-mediated depletion of CD4 T cells. Retrovirology. 2013;10:60 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200
Brinkmann K, Hombach A, Seeger J, Wagner Stippich D, Klubertz D, Kronke M, et al. Second mitochondria-derived activator of caspase (SMAC) mimetic potentiates tumor susceptibility toward natural killer cell-mediated killing. Leuk Lymphoma. 2014;55:645-51 pubmed publisher
  • western blot; mouse; 1:500
Huang S, Scruggs A, Donaghy J, Horowitz J, Zaslona Z, Przybranowski S, et al. Histone modifications are responsible for decreased Fas expression and apoptosis resistance in fibrotic lung fibroblasts. Cell Death Dis. 2013;4:e621 pubmed publisher
  • blocking or activating experiments; human
Li Y, Shively J. CEACAM1 regulates Fas-mediated apoptosis in Jurkat T-cells via its interaction with ?-catenin. Exp Cell Res. 2013;319:1061-72 pubmed publisher
  • blocking or activating experiments; human
Ajayi I, Sisson T, Higgins P, Booth A, Sagana R, Huang S, et al. X-linked inhibitor of apoptosis regulates lung fibroblast resistance to Fas-mediated apoptosis. Am J Respir Cell Mol Biol. 2013;49:86-95 pubmed publisher
  • flow cytometry; bovine; 1:25
  • flow cytometry; human; 1:25
Duncan A, Forcina J, Birt A, Townson D. Estrous cycle-dependent changes of Fas expression in the bovine corpus luteum: influence of keratin 8/18 intermediate filaments and cytokines. Reprod Biol Endocrinol. 2012;10:90 pubmed publisher
  • blocking or activating experiments; human; 100 ng/ml
Inoue S, Browne G, Melino G, Cohen G. Ordering of caspases in cells undergoing apoptosis by the intrinsic pathway. Cell Death Differ. 2009;16:1053-61 pubmed publisher
Dakic A, DiVito K, Fang S, Suprynowicz F, Gaur A, Li X, et al. ROCK inhibitor reduces Myc-induced apoptosis and mediates immortalization of human keratinocytes. Oncotarget. 2016;7:66740-66753 pubmed publisher
Chen C, Wu M, Huang Y, Lin P, Shih C, Lin F, et al. Iridovirus CARD Protein Inhibits Apoptosis through Intrinsic and Extrinsic Pathways. PLoS ONE. 2015;10:e0129071 pubmed publisher
Honjo K, Kubagawa Y, Kearney J, Kubagawa H. Unique ligand-binding property of the human IgM Fc receptor. J Immunol. 2015;194:1975-82 pubmed publisher
Kubagawa Y, Honjo K, Kang D, Kubagawa H. Monoclonal antibodies specific for human IgM Fc receptor inhibit ligand-binding activity. Monoclon Antib Immunodiagn Immunother. 2014;33:393-400 pubmed publisher
Benard A, Janssen C, van den Elsen P, van Eggermond M, Hoon D, Van De Velde C, et al. Chromatin status of apoptosis genes correlates with sensitivity to chemo-, immune- and radiation therapy in colorectal cancer cell lines. Apoptosis. 2014;19:1769-78 pubmed publisher
Popescu I, Drummond M, Gama L, Coon T, Merlo C, Wise R, et al. Activation-induced cell death drives profound lung CD4(+) T-cell depletion in HIV-associated chronic obstructive pulmonary disease. Am J Respir Crit Care Med. 2014;190:744-55 pubmed publisher
Mace P, Riedl S, Salvesen G. Caspase enzymology and activation mechanisms. Methods Enzymol. 2014;544:161-78 pubmed publisher
Al Ejeh F, Staudacher A, Smyth D, Darby J, Denoyer D, Tsopelas C, et al. Postchemotherapy and tumor-selective targeting with the La-specific DAB4 monoclonal antibody relates to apoptotic cell clearance. J Nucl Med. 2014;55:772-9 pubmed publisher
Jia S, Parodo J, Charbonney E, Tsang J, Jia S, Rotstein O, et al. Activated neutrophils induce epithelial cell apoptosis through oxidant-dependent tyrosine dephosphorylation of caspase-8. Am J Pathol. 2014;184:1030-1040 pubmed publisher
Das A, Ganesh K, Khanna S, Sen C, Roy S. Engulfment of apoptotic cells by macrophages: a role of microRNA-21 in the resolution of wound inflammation. J Immunol. 2014;192:1120-9 pubmed publisher
Chopra A, Anderson A, Giardina C. Novel piperazine-based compounds inhibit microtubule dynamics and sensitize colon cancer cells to tumor necrosis factor-induced apoptosis. J Biol Chem. 2014;289:2978-91 pubmed publisher
Geering B, Stoeckle C, Rozman S, Oberson K, Benarafa C, Simon H. DAPK2 positively regulates motility of neutrophils and eosinophils in response to intermediary chemoattractants. J Leukoc Biol. 2014;95:293-303 pubmed publisher
Gao J, Sana R, Calder V, Calonge M, Lee W, Wheeler L, et al. Mitochondrial permeability transition pore in inflammatory apoptosis of human conjunctival epithelial cells and T cells: effect of cyclosporin A. Invest Ophthalmol Vis Sci. 2013;54:4717-33 pubmed publisher
Wise J, Berkova Z, Mathur R, Zhu H, Braun F, Tao R, et al. Nucleolin inhibits Fas ligand binding and suppresses Fas-mediated apoptosis in vivo via a surface nucleolin-Fas complex. Blood. 2013;121:4729-39 pubmed publisher
Rippo M, Babini L, Prattichizzo F, Graciotti L, Fulgenzi G, Tomassoni Ardori F, et al. Low FasL levels promote proliferation of human bone marrow-derived mesenchymal stem cells, higher levels inhibit their differentiation into adipocytes. Cell Death Dis. 2013;4:e594 pubmed publisher
Nho R, Peterson M, Hergert P, Henke C. FoxO3a (Forkhead Box O3a) deficiency protects Idiopathic Pulmonary Fibrosis (IPF) fibroblasts from type I polymerized collagen matrix-induced apoptosis via caveolin-1 (cav-1) and Fas. PLoS ONE. 2013;8:e61017 pubmed publisher
Backman L, Danielson P. Akt-mediated anti-apoptotic effects of substance P in Anti-Fas-induced apoptosis of human tenocytes. J Cell Mol Med. 2013;17:723-33 pubmed publisher
Bryce S, Avlasevich S, Bemis J, Tate M, Walmsley R, Saad F, et al. Flow cytometric 96-well microplate-based in vitro micronucleus assay with human TK6 cells: protocol optimization and transferability assessment. Environ Mol Mutagen. 2013;54:180-94 pubmed publisher
Kastamoulas M, Chondrogiannis G, Kanavaros P, Vartholomatos G, Bai M, Briasoulis E, et al. Cytokine effects on cell survival and death of A549 lung carcinoma cells. Cytokine. 2013;61:816-25 pubmed publisher
Seidelin J, Coskun M, Vainer B, Riis L, Soendergaard C, Nielsen O. ERK controls epithelial cell death receptor signalling and cellular FLICE-like inhibitory protein (c-FLIP) in ulcerative colitis. J Mol Med (Berl). 2013;91:839-49 pubmed publisher
Faccenda D, Tan C, Seraphim A, Duchen M, Campanella M. IF1 limits the apoptotic-signalling cascade by preventing mitochondrial remodelling. Cell Death Differ. 2013;20:686-97 pubmed publisher
De Miguel D, Basañez G, Sanchez D, Malo P, Marzo I, Larrad L, et al. Liposomes decorated with Apo2L/TRAIL overcome chemoresistance of human hematologic tumor cells. Mol Pharm. 2013;10:893-904 pubmed publisher
Stenberg A, Sehlin J, Oldenborg P. Neutrophil apoptosis is associated with loss of signal regulatory protein alpha (SIRP?) from the cell surface. J Leukoc Biol. 2013;93:403-12 pubmed publisher
Pettigrew C, Clerkin J, Cotter T. DUOX enzyme activity promotes AKT signalling in prostate cancer cells. Anticancer Res. 2012;32:5175-81 pubmed
Perinpanayagam M, Beauchamp E, Martin D, Sim J, Yap M, Berthiaume L. Regulation of co- and post-translational myristoylation of proteins during apoptosis: interplay of N-myristoyltransferases and caspases. FASEB J. 2013;27:811-21 pubmed publisher
Förster A, Falcone F, Gibbs B, Preussner L, Fiebig B, Altunok H, et al. Anti-Fas/CD95 and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) differentially regulate apoptosis in normal and neoplastic human basophils. Leuk Lymphoma. 2013;54:835-42 pubmed publisher
Soto P, Karris M, Spina C, Richman D, Varki A. Cell-intrinsic mechanism involving Siglec-5 associated with divergent outcomes of HIV-1 infection in human and chimpanzee CD4 T cells. J Mol Med (Berl). 2013;91:261-70 pubmed publisher
Qian H, Xia L, Ling P, Waxman S, Jing Y. CD44 ligation with A3D8 antibody induces apoptosis in acute myeloid leukemia cells through binding to CD44s and clustering lipid rafts. Cancer Biol Ther. 2012;13:1276-83 pubmed publisher
Markwick L, Clements D, Roberts M, Ceresa C, Knox A, Johnson S. CCR3 induced-p42/44 MAPK activation protects against staurosporine induced-DNA fragmentation but not apoptosis in airway smooth muscle cells. Clin Exp Allergy. 2012;42:1040-50 pubmed publisher
Izawa T, Zou W, Chappel J, Ashley J, Feng X, Teitelbaum S. c-Src links a RANK/?v?3 integrin complex to the osteoclast cytoskeleton. Mol Cell Biol. 2012;32:2943-53 pubmed publisher
Steiert A, Reimers K, Burke W, Zapf A, Vogt P. Covalent vectored binding of functional proteins by bifunctional crosslinking at silicone interfaces. J Biomed Mater Res A. 2012;100:1248-55 pubmed publisher
Honjo K, Kubagawa Y, Kubagawa H. Is Toso an antiapoptotic protein or an Fc receptor for IgM?. Blood. 2012;119:1789-90 pubmed publisher
Chodorge M, Zuger S, Stirnimann C, Briand C, Jermutus L, Grutter M, et al. A series of Fas receptor agonist antibodies that demonstrate an inverse correlation between affinity and potency. Cell Death Differ. 2012;19:1187-95 pubmed publisher
Iglesias Guimarais V, Gil Guiñón E, Gabernet G, García Belinchón M, Sánchez Osuna M, Casanelles E, et al. Apoptotic DNA degradation into oligonucleosomal fragments, but not apoptotic nuclear morphology, relies on a cytosolic pool of DFF40/CAD endonuclease. J Biol Chem. 2012;287:7766-79 pubmed publisher
Hsu A, Dowdell K, Davis J, Niemela J, Anderson S, Shaw P, et al. Autoimmune lymphoproliferative syndrome due to FAS mutations outside the signal-transducing death domain: molecular mechanisms and clinical penetrance. Genet Med. 2012;14:81-9 pubmed publisher
Arabanian L, Kujawski S, Habermann I, Ehninger G, Kiani A. Regulation of fas/fas ligand-mediated apoptosis by nuclear factor of activated T cells in megakaryocytes. Br J Haematol. 2012;156:523-34 pubmed publisher
Horowitz J, Ajayi I, Kulasekaran P, Rogers D, White J, Townsend S, et al. Survivin expression induced by endothelin-1 promotes myofibroblast resistance to apoptosis. Int J Biochem Cell Biol. 2012;44:158-69 pubmed publisher
Martin D, Ahpin C, Heit R, Perinpanayagam M, Yap M, Veldhoen R, et al. Tandem reporter assay for myristoylated proteins post-translationally (TRAMPP) identifies novel substrates for post-translational myristoylation: PKC?, a case study. FASEB J. 2012;26:13-28 pubmed publisher
Steiert A, Sendler D, Burke W, Choi C, Reimers K, Vogt P. Attack the tumor counterattack-c-FLIP expression in Jurkat-T-cells protects against apoptosis induced by coculture with SW620 colorectal adenocarcinoma cells. J Surg Res. 2012;176:133-40 pubmed publisher
Cummins N, Klicpera A, Sainski A, Bren G, Khosla S, Westendorf J, et al. Human immunodeficiency virus envelope protein Gp120 induces proliferation but not apoptosis in osteoblasts at physiologic concentrations. PLoS ONE. 2011;6:e24876 pubmed publisher
Li H, Pauza C. HIV envelope-mediated, CCR5/?4?7-dependent killing of CD4-negative ?? T cells which are lost during progression to AIDS. Blood. 2011;118:5824-31 pubmed publisher
Tsai C, Huang W, Chen C, Hsieh C, Lin Y, Chen S, et al. Glycogen synthase kinase-3 facilitates con a-induced IFN-?-- mediated immune hepatic injury. J Immunol. 2011;187:3867-77 pubmed publisher
Cerella C, Sobolewski C, Chateauvieux S, Henry E, Schnekenburger M, Ghelfi J, et al. COX-2 inhibitors block chemotherapeutic agent-induced apoptosis prior to commitment in hematopoietic cancer cells. Biochem Pharmacol. 2011;82:1277-90 pubmed publisher
Zhou Y, Adolfs Y, Pijnappel W, Fuller S, van der Schors R, Li K, et al. MICAL-1 is a negative regulator of MST-NDR kinase signaling and apoptosis. Mol Cell Biol. 2011;31:3603-15 pubmed publisher
Jeon H, Zheng L, Lee S, Lee W, Park N, Park J, et al. Comparative analysis of the role of small G proteins in cell migration and cell death: cytoprotective and promigratory effects of RalA. Exp Cell Res. 2011;317:2007-18 pubmed publisher
Swindall A, Bellis S. Sialylation of the Fas death receptor by ST6Gal-I provides protection against Fas-mediated apoptosis in colon carcinoma cells. J Biol Chem. 2011;286:22982-90 pubmed publisher
Geng L, Zhu B, Dai B, Sui C, Xu F, Kan T, et al. A let-7/Fas double-negative feedback loop regulates human colon carcinoma cells sensitivity to Fas-related apoptosis. Biochem Biophys Res Commun. 2011;408:494-9 pubmed publisher
Sancho Martínez S, Piedrafita F, Cannata Andia J, Lopez Novoa J, Lopez Hernandez F. Necrotic concentrations of cisplatin activate the apoptotic machinery but inhibit effector caspases and interfere with the execution of apoptosis. Toxicol Sci. 2011;122:73-85 pubmed publisher
Hofmann Bowman M, Heydemann A, Gawdzik J, Shilling R, Camoretti Mercado B. Transgenic expression of human S100A12 induces structural airway abnormalities and limited lung inflammation in a mouse model of allergic inflammation. Clin Exp Allergy. 2011;41:878-89 pubmed publisher
Martínez Serra J, Maffiotte E, Martin J, Bex T, Navarro Palou M, Ros T, et al. Yondelis® (ET-743, Trabectedin) sensitizes cancer cell lines to CD95-mediated cell death: new molecular insight into the mechanism of action. Eur J Pharmacol. 2011;658:57-64 pubmed publisher
Muhlethaler Mottet A, Flahaut M, Bourloud K, Nardou K, Coulon A, Liberman J, et al. Individual caspase-10 isoforms play distinct and opposing roles in the initiation of death receptor-mediated tumour cell apoptosis. Cell Death Dis. 2011;2:e125 pubmed publisher
Crescenzi E, Pacifico F, Lavorgna A, De Palma R, D Aiuto E, Palumbo G, et al. NF-?B-dependent cytokine secretion controls Fas expression on chemotherapy-induced premature senescent tumor cells. Oncogene. 2011;30:2707-17 pubmed publisher
Li Z, Xu Q, Peng H, Cheng R, Sun Z, Ye Z. IFN-? enhances HOS and U2OS cell lines susceptibility to ?? T cell-mediated killing through the Fas/Fas ligand pathway. Int Immunopharmacol. 2011;11:496-503 pubmed publisher
Croft D, Crighton D, Samuel M, Lourenco F, Munro J, Wood J, et al. p53-mediated transcriptional regulation and activation of the actin cytoskeleton regulatory RhoC to LIMK2 signaling pathway promotes cell survival. Cell Res. 2011;21:666-82 pubmed publisher
Ashley J, McCoy E, Clements D, Shi Z, Chen T, Feng X. Development of cell-based high-throughput assays for the identification of inhibitors of receptor activator of nuclear factor-kappa B signaling. Assay Drug Dev Technol. 2011;9:40-9 pubmed publisher
Salvetti N, Stangaferro M, Palomar M, Alfaro N, Rey F, Gimeno E, et al. Cell proliferation and survival mechanisms underlying the abnormal persistence of follicular cysts in bovines with cystic ovarian disease induced by ACTH. Anim Reprod Sci. 2010;122:98-110 pubmed publisher
Akimzhanov A, Wang X, Sun J, Boehning D. T-cell receptor complex is essential for Fas signal transduction. Proc Natl Acad Sci U S A. 2010;107:15105-10 pubmed publisher
Sagan D, Jermnim N, Tangvarasittichai O. CD95 is not functional in human erythrocytes. Int J Lab Hematol. 2010;32:e244-7 pubmed publisher
Um H, Kwon T. Protective effect of melatonin on oxaliplatin-induced apoptosis through sustained Mcl-1 expression and anti-oxidant action in renal carcinoma Caki cells. J Pineal Res. 2010;49:283-90 pubmed publisher
Kohler R, Schmitz D, Cornils H, Hemmings B, Hergovich A. Differential NDR/LATS interactions with the human MOB family reveal a negative role for human MOB2 in the regulation of human NDR kinases. Mol Cell Biol. 2010;30:4507-20 pubmed publisher
Cho H, Park S, Hwang E, Baek K, Kim I, Nam I, et al. Gadd45b mediates Fas-induced apoptosis by enhancing the interaction between p38 and retinoblastoma tumor suppressor. J Biol Chem. 2010;285:25500-5 pubmed publisher
Wen K, Damania B. Hsp90 and Hsp40/Erdj3 are required for the expression and anti-apoptotic function of KSHV K1. Oncogene. 2010;29:3532-44 pubmed publisher
Woodcock J, Ma Y, Coolen C, Pham D, Jones C, Lopez A, et al. Sphingosine and FTY720 directly bind pro-survival 14-3-3 proteins to regulate their function. Cell Signal. 2010;22:1291-9 pubmed publisher
Kim Y, Lee H, Jang C, Kim H, Cho Y. Knockdown of RCAN1.4 Increases Susceptibility to FAS-mediated and DNA-damage-induced Apoptosis by Upregulation of p53 Expression. Korean J Physiol Pharmacol. 2009;13:483-9 pubmed publisher
Xu Z, Ding T, Haridas V, Connolly F, Gutterman J. Avicin D, a plant triterpenoid, induces cell apoptosis by recruitment of Fas and downstream signaling molecules into lipid rafts. PLoS ONE. 2009;4:e8532 pubmed publisher
Yap M, Kostiuk M, Martin D, Perinpanayagam M, Hak P, Siddam A, et al. Rapid and selective detection of fatty acylated proteins using omega-alkynyl-fatty acids and click chemistry. J Lipid Res. 2010;51:1566-80 pubmed publisher
Eeva J, Nuutinen U, Ropponen A, Mättö M, Eray M, Pellinen R, et al. The involvement of mitochondria and the caspase-9 activation pathway in rituximab-induced apoptosis in FL cells. Apoptosis. 2009;14:687-98 pubmed publisher
Engedal N, Auberger P, Blomhoff H. Retinoic acid regulates Fas-induced apoptosis in Jurkat T cells: reversal of mitogen-mediated repression of Fas DISC assembly. J Leukoc Biol. 2009;85:469-80 pubmed publisher
Wedemeyer I, Bechmann L, Odenthal M, Jochum C, Marquitan G, Drebber U, et al. Adiponectin inhibits steatotic CD95/Fas up-regulation by hepatocytes: therapeutic implications for hepatitis C. J Hepatol. 2009;50:140-9 pubmed publisher
Pop C, Salvesen G, Scott F. Caspase assays: identifying caspase activity and substrates in vitro and in vivo. Methods Enzymol. 2008;446:351-67 pubmed publisher
Ruan W, Lee C, Desbarats J. A novel juxtamembrane domain in tumor necrosis factor receptor superfamily molecules activates Rac1 and controls neurite growth. Mol Biol Cell. 2008;19:3192-202 pubmed publisher
Izquierdo J. Fas splicing regulation during early apoptosis is linked to caspase-mediated cleavage of U2AF65. Mol Biol Cell. 2008;19:3299-307 pubmed publisher
De Zio D, Ferraro E, D Amelio M, Simoni V, Bordi M, Soroldoni D, et al. Faf1 is expressed during neurodevelopment and is involved in Apaf1-dependent caspase-3 activation in proneural cells. Cell Mol Life Sci. 2008;65:1780-90 pubmed publisher
Dash P, McCormick J, Thomson M, Johnstone A, Cartwright J, Whitley G. Fas ligand-induced apoptosis is regulated by nitric oxide through the inhibition of fas receptor clustering and the nitrosylation of protein kinase Cepsilon. Exp Cell Res. 2007;313:3421-31 pubmed
Yuneva M, Zamboni N, Oefner P, Sachidanandam R, Lazebnik Y. Deficiency in glutamine but not glucose induces MYC-dependent apoptosis in human cells. J Cell Biol. 2007;178:93-105 pubmed
Obermeier F, Hausmann M, Kellermeier S, Kiessling S, Strauch U, Duitman E, et al. IL-15 protects intestinal epithelial cells. Eur J Immunol. 2006;36:2691-9 pubmed
Petrovas C, Casazza J, Brenchley J, Price D, Gostick E, Adams W, et al. PD-1 is a regulator of virus-specific CD8+ T cell survival in HIV infection. J Exp Med. 2006;203:2281-92 pubmed
Krispin A, Bledi Y, Atallah M, Trahtemberg U, Verbovetski I, Nahari E, et al. Apoptotic cell thrombospondin-1 and heparin-binding domain lead to dendritic-cell phagocytic and tolerizing states. Blood. 2006;108:3580-9 pubmed
Li H, Wang A, Zhang R, Wei Y, Chen H, She Z, et al. A20 inhibits oxidized low-density lipoprotein-induced apoptosis through negative Fas/Fas ligand-dependent activation of caspase-8 and mitochondrial pathways in murine RAW264.7 macrophages. J Cell Physiol. 2006;208:307-18 pubmed
de Groot D, Timmer T, Spierings D, Le T, de Jong S, de Vries E. Indomethacin-induced activation of the death receptor-mediated apoptosis pathway circumvents acquired doxorubicin resistance in SCLC cells. Br J Cancer. 2005;92:1459-66 pubmed
Iordanov M, Kirsch J, Ryabinina O, Wong J, Spitz P, Korcheva V, et al. Recruitment of TRADD, FADD, and caspase 8 to double-stranded RNA-triggered death inducing signaling complexes (dsRNA-DISCs). Apoptosis. 2005;10:167-76 pubmed
Iordanov M, Ryabinina O, Schneider P, Magun B. Two mechanisms of caspase 9 processing in double-stranded RNA- and virus-triggered apoptosis. Apoptosis. 2005;10:153-66 pubmed
Ricci Vitiani L, Pedini F, Mollinari C, Condorelli G, Bonci D, Bez A, et al. Absence of caspase 8 and high expression of PED protect primitive neural cells from cell death. J Exp Med. 2004;200:1257-66 pubmed
product information
Catalog Number :
05-201
Subcategory :
Apoptosis & Cancer
Product Name :
Anti-Fas Antibody (human, activating), clone CH11
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P25445
Host Name :
Mouse
Antigen :
Fas (human
Clone :
CH11
Conjugate :
Affinity Purified
Isotype :
IgM
Product Description :
Anti-Fas Antibody (human, activating), clone CH11
Cross Reactivity :
Human
Background :
Fas/APO-1/CD95 (36 kDa) is a member of the tumor necrosis factor (TNF) receptor superfamily, a family of transmembrane receptors. Fas has been shown to be an important mediator of apoptotic cell death, as well as being involved in inflammation. Binding of the Fas ligand (Fas-L) induces trimerization of Fas in the target cell membrane. Activation of Fas causes the recruitment of Fas-associated protein with death domain (FADD) via interactions between the death domains of Fas and FADD. Procaspase 8 binds to Fas-bound FADD via interactions between the death effector domains (DED) of FADD and pro-caspase 8 leading to the activation of caspase 8. Activated caspase 8 cleaves (activates) other procaspases, in effect beginning a caspase cascade that ultimately leads to apoptosis. Caspases cleave nuclear lamins, causing the nucleus to break down and lose its normal structure. Fas-induced apoptosis can be effectively blocked at several stages by either FLICE-inhibitory protein (FLIP), by Bcl-2, or by the cytokine response modifier A (CrmA). Biological Activity The antibody demonstrates cytolytic activity on human cells that express Fas. Murine WR19L cells and L929 cells transfected with cDNA encoding human Fas undergo apoptosis in response to this antibody.
ALT Names :
APO-1 cell surface antigen;CD95 antigen;Fas (TNF receptor superfamily, member 6);Fas AMA;Fas antigen;apoptosis antigen 1;tumor necrosis factor receptor superfamily, member 6
Immunogen :
FS-7 (human diploid fibroblast cell line). Clone CH-11.
Specificity :
This antibody recognizes the human cell surface antigen Fas, Mr 43 kDa expressed in various human cells, including myeloid cells, T lympho-blastoid cells, and diploid fibroblasts.
Package Size :
50 µg
Uses :
Flow Cytometry;Immunocytochemistry;Western Blotting
Storage :
Stable for 1 year at -20°C from date of receipt. For maximum recovery of the product, centrifuge the original vial prior to removing the cap.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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